Experiment: Mc formate, casamino free, with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04410 and MMP_RS04415 are separated by 82 nucleotides MMP_RS04415 and MMP_RS04420 are separated by 254 nucleotides MMP_RS04420 and MMP_RS04425 are separated by 62 nucleotides
MMP_RS04410: MMP_RS04410 - hypothetical protein, at 839,216 to 839,815
_RS04410
MMP_RS04415: MMP_RS04415 - peptide-methionine (S)-S-oxide reductase MsrA, at 839,898 to 840,371
_RS04415
MMP_RS04420: MMP_RS04420 - LysE family translocator, at 840,626 to 841,249
_RS04420
MMP_RS04425: MMP_RS04425 - APC family permease, at 841,312 to 842,565
_RS04425
Position (kb) 840
841
842 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 839.643 kb on + strand, within MMP_RS04410 at 839.663 kb on - strand, within MMP_RS04410 at 839.748 kb on - strand, within MMP_RS04410 at 839.819 kb on + strand at 839.843 kb on + strand at 839.849 kb on - strand at 839.850 kb on + strand at 839.863 kb on - strand at 839.870 kb on + strand at 839.876 kb on + strand at 839.919 kb on + strand at 839.936 kb on + strand at 839.936 kb on + strand at 840.045 kb on - strand, within MMP_RS04415 at 840.045 kb on - strand, within MMP_RS04415 at 840.046 kb on + strand, within MMP_RS04415 at 840.086 kb on + strand, within MMP_RS04415 at 840.122 kb on - strand, within MMP_RS04415 at 840.122 kb on - strand, within MMP_RS04415 at 840.123 kb on + strand, within MMP_RS04415 at 840.148 kb on + strand, within MMP_RS04415 at 840.153 kb on - strand, within MMP_RS04415 at 840.156 kb on - strand, within MMP_RS04415 at 840.157 kb on + strand, within MMP_RS04415 at 840.172 kb on + strand, within MMP_RS04415 at 840.193 kb on + strand, within MMP_RS04415 at 840.197 kb on - strand, within MMP_RS04415 at 840.360 kb on + strand at 840.362 kb on - strand at 840.392 kb on - strand at 840.393 kb on + strand at 840.422 kb on + strand at 840.458 kb on - strand at 840.463 kb on + strand at 840.463 kb on + strand at 840.485 kb on - strand at 840.486 kb on + strand at 840.487 kb on - strand at 840.489 kb on - strand at 840.490 kb on + strand at 840.490 kb on + strand at 840.501 kb on - strand at 840.502 kb on + strand at 840.502 kb on + strand at 840.510 kb on + strand at 840.520 kb on - strand at 840.582 kb on + strand at 840.590 kb on - strand at 840.590 kb on - strand at 840.590 kb on - strand at 840.590 kb on - strand at 840.591 kb on + strand at 840.595 kb on + strand at 840.599 kb on + strand at 840.637 kb on - strand at 840.650 kb on - strand at 840.676 kb on - strand at 840.676 kb on - strand at 840.677 kb on + strand at 840.677 kb on + strand at 840.699 kb on - strand, within MMP_RS04420 at 840.791 kb on + strand, within MMP_RS04420 at 840.937 kb on - strand, within MMP_RS04420 at 841.071 kb on - strand, within MMP_RS04420 at 841.155 kb on - strand, within MMP_RS04420 at 841.182 kb on + strand, within MMP_RS04420 at 841.300 kb on + strand at 841.311 kb on - strand at 841.311 kb on - strand at 841.311 kb on - strand at 841.337 kb on + strand at 841.338 kb on - strand at 841.338 kb on - strand at 841.339 kb on + strand at 841.339 kb on + strand at 841.343 kb on - strand at 841.344 kb on + strand at 841.353 kb on - strand at 841.388 kb on - strand at 841.389 kb on + strand at 841.401 kb on + strand at 841.401 kb on + strand at 841.405 kb on - strand at 841.411 kb on + strand at 841.490 kb on - strand, within MMP_RS04425 at 841.490 kb on - strand, within MMP_RS04425 at 841.532 kb on - strand, within MMP_RS04425 at 841.533 kb on + strand, within MMP_RS04425 at 841.573 kb on - strand, within MMP_RS04425 at 841.574 kb on + strand, within MMP_RS04425 at 841.576 kb on - strand, within MMP_RS04425 at 841.576 kb on - strand, within MMP_RS04425 at 841.577 kb on + strand, within MMP_RS04425 at 841.577 kb on + strand, within MMP_RS04425 at 841.577 kb on + strand, within MMP_RS04425 at 841.581 kb on + strand, within MMP_RS04425 at 841.581 kb on + strand, within MMP_RS04425 at 841.583 kb on - strand, within MMP_RS04425 at 841.584 kb on + strand, within MMP_RS04425 at 841.647 kb on + strand, within MMP_RS04425 at 841.678 kb on - strand, within MMP_RS04425 at 841.679 kb on + strand, within MMP_RS04425 at 841.692 kb on + strand, within MMP_RS04425 at 841.709 kb on - strand, within MMP_RS04425 at 841.736 kb on - strand, within MMP_RS04425 at 841.767 kb on + strand, within MMP_RS04425 at 841.773 kb on - strand, within MMP_RS04425 at 841.778 kb on - strand, within MMP_RS04425 at 841.785 kb on + strand, within MMP_RS04425 at 841.785 kb on + strand, within MMP_RS04425 at 841.785 kb on + strand, within MMP_RS04425 at 841.847 kb on - strand, within MMP_RS04425 at 841.847 kb on - strand, within MMP_RS04425 at 841.851 kb on - strand, within MMP_RS04425 at 841.862 kb on - strand, within MMP_RS04425 at 841.863 kb on + strand, within MMP_RS04425 at 841.867 kb on - strand, within MMP_RS04425 at 841.897 kb on + strand, within MMP_RS04425 at 841.970 kb on - strand, within MMP_RS04425 at 841.971 kb on + strand, within MMP_RS04425 at 841.973 kb on - strand, within MMP_RS04425 at 841.974 kb on + strand, within MMP_RS04425 at 841.974 kb on + strand, within MMP_RS04425 at 841.980 kb on + strand, within MMP_RS04425 at 841.992 kb on - strand, within MMP_RS04425 at 841.992 kb on - strand, within MMP_RS04425 at 841.993 kb on + strand, within MMP_RS04425 at 841.994 kb on - strand, within MMP_RS04425 at 842.031 kb on + strand, within MMP_RS04425 at 842.054 kb on - strand, within MMP_RS04425 at 842.055 kb on + strand, within MMP_RS04425 at 842.081 kb on - strand, within MMP_RS04425 at 842.081 kb on - strand, within MMP_RS04425 at 842.084 kb on - strand, within MMP_RS04425 at 842.116 kb on + strand, within MMP_RS04425 at 842.116 kb on + strand, within MMP_RS04425 at 842.117 kb on - strand, within MMP_RS04425 at 842.127 kb on - strand, within MMP_RS04425 at 842.130 kb on - strand, within MMP_RS04425 at 842.244 kb on - strand, within MMP_RS04425
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM Glycine remove 839,643 + MMP_RS04410 0.71 -0.0 839,663 - MMP_RS04410 0.74 +0.3 839,748 - MMP_RS04410 0.89 +0.2 839,819 + -0.4 839,843 + +0.4 839,849 - +0.1 839,850 + -0.0 839,863 - +0.5 839,870 + -0.5 839,876 + +1.2 839,919 + -0.5 839,936 + -0.2 839,936 + -0.6 840,045 - MMP_RS04415 0.31 +1.3 840,045 - MMP_RS04415 0.31 +0.5 840,046 + MMP_RS04415 0.31 -0.5 840,086 + MMP_RS04415 0.40 +0.4 840,122 - MMP_RS04415 0.47 +0.1 840,122 - MMP_RS04415 0.47 -0.1 840,123 + MMP_RS04415 0.47 -1.1 840,148 + MMP_RS04415 0.53 -0.9 840,153 - MMP_RS04415 0.54 +0.5 840,156 - MMP_RS04415 0.54 +0.0 840,157 + MMP_RS04415 0.55 +0.6 840,172 + MMP_RS04415 0.58 +0.2 840,193 + MMP_RS04415 0.62 +0.7 840,197 - MMP_RS04415 0.63 -0.4 840,360 + +1.3 840,362 - +0.1 840,392 - -3.1 840,393 + -1.1 840,422 + -1.8 840,458 - +0.2 840,463 + -1.1 840,463 + -0.1 840,485 - +0.1 840,486 + +0.3 840,487 - -0.7 840,489 - +1.2 840,490 + +0.4 840,490 + +0.4 840,501 - -0.6 840,502 + +0.5 840,502 + -0.5 840,510 + -0.3 840,520 - -0.8 840,582 + +0.8 840,590 - -1.1 840,590 - -1.5 840,590 - +0.4 840,590 - +0.4 840,591 + +0.3 840,595 + +0.6 840,599 + -1.8 840,637 - -0.6 840,650 - -0.5 840,676 - -0.0 840,676 - +0.2 840,677 + +0.6 840,677 + +0.6 840,699 - MMP_RS04420 0.12 -1.1 840,791 + MMP_RS04420 0.26 -0.8 840,937 - MMP_RS04420 0.50 +0.7 841,071 - MMP_RS04420 0.71 -0.8 841,155 - MMP_RS04420 0.85 -2.8 841,182 + MMP_RS04420 0.89 +0.3 841,300 + +0.7 841,311 - -1.6 841,311 - -0.1 841,311 - +1.1 841,337 + +0.7 841,338 - -1.3 841,338 - +0.5 841,339 + +0.3 841,339 + -0.6 841,343 - +0.9 841,344 + +0.6 841,353 - +0.8 841,388 - -0.1 841,389 + +0.7 841,401 + +1.1 841,401 + +0.5 841,405 - -0.1 841,411 + -0.9 841,490 - MMP_RS04425 0.14 +0.3 841,490 - MMP_RS04425 0.14 -0.1 841,532 - MMP_RS04425 0.18 +0.2 841,533 + MMP_RS04425 0.18 +0.3 841,573 - MMP_RS04425 0.21 +0.1 841,574 + MMP_RS04425 0.21 +0.0 841,576 - MMP_RS04425 0.21 -0.8 841,576 - MMP_RS04425 0.21 -0.8 841,577 + MMP_RS04425 0.21 -0.0 841,577 + MMP_RS04425 0.21 -0.3 841,577 + MMP_RS04425 0.21 -1.0 841,581 + MMP_RS04425 0.21 -0.3 841,581 + MMP_RS04425 0.21 +0.6 841,583 - MMP_RS04425 0.22 +0.8 841,584 + MMP_RS04425 0.22 -0.0 841,647 + MMP_RS04425 0.27 +0.6 841,678 - MMP_RS04425 0.29 -0.3 841,679 + MMP_RS04425 0.29 -0.2 841,692 + MMP_RS04425 0.30 +0.6 841,709 - MMP_RS04425 0.32 -0.4 841,736 - MMP_RS04425 0.34 +0.1 841,767 + MMP_RS04425 0.36 -0.5 841,773 - MMP_RS04425 0.37 +0.8 841,778 - MMP_RS04425 0.37 -0.4 841,785 + MMP_RS04425 0.38 +0.2 841,785 + MMP_RS04425 0.38 +0.8 841,785 + MMP_RS04425 0.38 -2.7 841,847 - MMP_RS04425 0.43 +1.2 841,847 - MMP_RS04425 0.43 +0.5 841,851 - MMP_RS04425 0.43 -0.7 841,862 - MMP_RS04425 0.44 +0.3 841,863 + MMP_RS04425 0.44 -0.1 841,867 - MMP_RS04425 0.44 +0.3 841,897 + MMP_RS04425 0.47 +0.6 841,970 - MMP_RS04425 0.52 -0.6 841,971 + MMP_RS04425 0.53 -0.1 841,973 - MMP_RS04425 0.53 -0.1 841,974 + MMP_RS04425 0.53 -0.6 841,974 + MMP_RS04425 0.53 +0.6 841,980 + MMP_RS04425 0.53 +0.6 841,992 - MMP_RS04425 0.54 +0.9 841,992 - MMP_RS04425 0.54 +0.4 841,993 + MMP_RS04425 0.54 -0.8 841,994 - MMP_RS04425 0.54 -0.1 842,031 + MMP_RS04425 0.57 +2.6 842,054 - MMP_RS04425 0.59 +0.4 842,055 + MMP_RS04425 0.59 +0.9 842,081 - MMP_RS04425 0.61 -0.2 842,081 - MMP_RS04425 0.61 +0.1 842,084 - MMP_RS04425 0.62 +0.6 842,116 + MMP_RS04425 0.64 -0.3 842,116 + MMP_RS04425 0.64 -0.2 842,117 - MMP_RS04425 0.64 +0.9 842,127 - MMP_RS04425 0.65 +1.0 842,130 - MMP_RS04425 0.65 +0.5 842,244 - MMP_RS04425 0.74 -0.5
Or see this region's nucleotide sequence