Experiment: Mc formate, casamino free, with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02525 and MMP_RS02530 are separated by 732 nucleotides MMP_RS02530 and MMP_RS02535 overlap by 4 nucleotides MMP_RS02535 and MMP_RS02540 are separated by 148 nucleotides
MMP_RS02525: MMP_RS02525 - DUF2254 domain-containing protein, at 471,143 to 472,984
_RS02525
MMP_RS02530: MMP_RS02530 - type II toxin-antitoxin system mRNA interferase toxin RelE/StbE family, at 473,717 to 474,019
_RS02530
MMP_RS02535: MMP_RS02535 - DUF2683 family protein, at 474,016 to 474,264
_RS02535
MMP_RS02540: MMP_RS02540 - DUF2283 domain-containing protein, at 474,413 to 474,790
_RS02540
Position (kb) 473
474
475 Strain fitness (log2 ratio) -2
-1
0
1 at 472.773 kb on + strand, within MMP_RS02525 at 472.831 kb on + strand at 472.837 kb on - strand at 472.862 kb on + strand at 472.862 kb on + strand at 472.862 kb on + strand at 472.911 kb on - strand at 472.911 kb on - strand at 472.965 kb on + strand at 473.003 kb on + strand at 473.008 kb on - strand at 473.095 kb on - strand at 473.167 kb on - strand at 473.167 kb on - strand at 473.168 kb on + strand at 473.213 kb on + strand at 473.379 kb on - strand at 473.445 kb on + strand at 473.450 kb on - strand at 473.460 kb on - strand at 473.650 kb on + strand at 473.754 kb on - strand, within MMP_RS02530 at 473.755 kb on + strand, within MMP_RS02530 at 473.776 kb on + strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.788 kb on + strand, within MMP_RS02530 at 473.789 kb on - strand, within MMP_RS02530 at 473.838 kb on - strand, within MMP_RS02530 at 473.948 kb on + strand, within MMP_RS02530 at 473.989 kb on - strand at 474.169 kb on - strand at 474.284 kb on + strand at 474.306 kb on - strand at 474.319 kb on - strand at 474.323 kb on - strand at 474.324 kb on + strand at 474.367 kb on + strand at 474.368 kb on - strand at 474.369 kb on + strand at 474.374 kb on - strand at 474.375 kb on + strand at 474.657 kb on + strand, within MMP_RS02540 at 474.714 kb on - strand, within MMP_RS02540 at 474.742 kb on + strand, within MMP_RS02540 at 474.742 kb on + strand, within MMP_RS02540 at 474.746 kb on - strand, within MMP_RS02540 at 474.746 kb on - strand, within MMP_RS02540 at 474.746 kb on - strand, within MMP_RS02540 at 474.747 kb on + strand, within MMP_RS02540 at 474.766 kb on + strand at 474.814 kb on - strand at 474.814 kb on - strand at 474.839 kb on - strand at 474.840 kb on + strand at 474.841 kb on - strand at 474.841 kb on - strand at 474.846 kb on - strand at 474.847 kb on + strand at 474.847 kb on + strand at 474.852 kb on + strand at 474.880 kb on + strand at 474.883 kb on - strand at 474.900 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM Glycine remove 472,773 + MMP_RS02525 0.88 -0.0 472,831 + +1.2 472,837 - +1.2 472,862 + -1.6 472,862 + +0.5 472,862 + -0.7 472,911 - +1.0 472,911 - +0.5 472,965 + +0.0 473,003 + +0.1 473,008 - -0.4 473,095 - +0.6 473,167 - -0.1 473,167 - +1.3 473,168 + -0.4 473,213 + +0.4 473,379 - +0.9 473,445 + +1.1 473,450 - +0.9 473,460 - +0.9 473,650 + +0.5 473,754 - MMP_RS02530 0.12 -0.7 473,755 + MMP_RS02530 0.13 -2.2 473,776 + MMP_RS02530 0.19 +0.7 473,787 - MMP_RS02530 0.23 +0.6 473,787 - MMP_RS02530 0.23 -1.8 473,787 - MMP_RS02530 0.23 -0.1 473,788 + MMP_RS02530 0.23 +0.3 473,789 - MMP_RS02530 0.24 -1.9 473,838 - MMP_RS02530 0.40 -0.7 473,948 + MMP_RS02530 0.76 -1.2 473,989 - +1.0 474,169 - -1.2 474,284 + -2.4 474,306 - -0.2 474,319 - -1.7 474,323 - +0.3 474,324 + +0.8 474,367 + -0.4 474,368 - -2.3 474,369 + -0.0 474,374 - +1.3 474,375 + -0.6 474,657 + MMP_RS02540 0.65 +1.1 474,714 - MMP_RS02540 0.80 +0.3 474,742 + MMP_RS02540 0.87 -0.6 474,742 + MMP_RS02540 0.87 +0.7 474,746 - MMP_RS02540 0.88 -0.1 474,746 - MMP_RS02540 0.88 -1.3 474,746 - MMP_RS02540 0.88 -1.6 474,747 + MMP_RS02540 0.88 +0.1 474,766 + -1.6 474,814 - -0.1 474,814 - +0.7 474,839 - -0.1 474,840 + +0.4 474,841 - -0.5 474,841 - +0.7 474,846 - -0.2 474,847 + +0.7 474,847 + +1.0 474,852 + +1.0 474,880 + -0.0 474,883 - -0.1 474,900 + -0.3
Or see this region's nucleotide sequence