Experiment: Mc formate, casamino free, with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00055 and MMP_RS00060 are separated by 127 nucleotides MMP_RS00060 and MMP_RS00065 are separated by 381 nucleotides
MMP_RS00055: MMP_RS00055 - DUF2254 domain-containing protein, at 13,625 to 15,208
_RS00055
MMP_RS00060: MMP_RS00060 - 3-dehydroquinate synthase II, at 15,336 to 16,421
_RS00060
MMP_RS00065: MMP_RS00065 - geranylgeranylglyceryl/heptaprenylglyceryl phosphate synthase, at 16,803 to 17,573
_RS00065
Position (kb) 15
16
17 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 14.345 kb on - strand, within MMP_RS00055 at 14.420 kb on - strand, within MMP_RS00055 at 14.444 kb on - strand, within MMP_RS00055 at 14.448 kb on - strand, within MMP_RS00055 at 14.575 kb on - strand, within MMP_RS00055 at 14.576 kb on + strand, within MMP_RS00055 at 14.576 kb on + strand, within MMP_RS00055 at 14.576 kb on + strand, within MMP_RS00055 at 14.626 kb on + strand, within MMP_RS00055 at 14.742 kb on - strand, within MMP_RS00055 at 14.801 kb on + strand, within MMP_RS00055 at 14.801 kb on + strand, within MMP_RS00055 at 14.967 kb on + strand, within MMP_RS00055 at 15.046 kb on - strand, within MMP_RS00055 at 15.047 kb on + strand, within MMP_RS00055 at 15.131 kb on - strand at 15.172 kb on - strand at 15.173 kb on + strand at 15.177 kb on - strand at 15.177 kb on - strand at 15.234 kb on + strand at 15.237 kb on - strand at 15.237 kb on - strand at 15.238 kb on + strand at 15.242 kb on - strand at 15.242 kb on - strand at 15.242 kb on - strand at 15.246 kb on - strand at 15.247 kb on + strand at 15.247 kb on + strand at 15.247 kb on + strand at 15.253 kb on + strand at 15.267 kb on + strand at 15.279 kb on - strand at 15.280 kb on + strand at 15.515 kb on + strand, within MMP_RS00060 at 15.590 kb on + strand, within MMP_RS00060 at 16.082 kb on + strand, within MMP_RS00060 at 16.458 kb on + strand at 16.524 kb on - strand at 16.525 kb on + strand at 16.525 kb on + strand at 16.525 kb on + strand at 16.536 kb on - strand at 16.536 kb on - strand at 16.536 kb on - strand at 16.537 kb on + strand at 16.555 kb on - strand at 16.556 kb on + strand at 16.556 kb on + strand at 16.556 kb on + strand at 16.569 kb on - strand at 16.578 kb on - strand at 16.579 kb on + strand at 16.605 kb on + strand at 16.628 kb on + strand at 16.647 kb on + strand at 16.648 kb on - strand at 16.655 kb on + strand at 16.655 kb on + strand at 16.682 kb on - strand at 16.683 kb on + strand at 16.683 kb on + strand at 16.737 kb on + strand at 16.782 kb on + strand at 16.791 kb on + strand at 16.883 kb on + strand at 17.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM Glycine remove 14,345 - MMP_RS00055 0.45 -0.4 14,420 - MMP_RS00055 0.50 -0.6 14,444 - MMP_RS00055 0.52 -0.4 14,448 - MMP_RS00055 0.52 +0.6 14,575 - MMP_RS00055 0.60 +0.3 14,576 + MMP_RS00055 0.60 +0.4 14,576 + MMP_RS00055 0.60 +0.8 14,576 + MMP_RS00055 0.60 +0.2 14,626 + MMP_RS00055 0.63 +0.1 14,742 - MMP_RS00055 0.71 +0.2 14,801 + MMP_RS00055 0.74 +1.3 14,801 + MMP_RS00055 0.74 +0.6 14,967 + MMP_RS00055 0.85 +0.1 15,046 - MMP_RS00055 0.90 +0.1 15,047 + MMP_RS00055 0.90 +0.5 15,131 - -0.1 15,172 - -0.1 15,173 + -0.8 15,177 - +0.7 15,177 - +0.3 15,234 + +0.1 15,237 - +0.2 15,237 - +0.3 15,238 + -0.8 15,242 - -0.8 15,242 - +0.6 15,242 - -0.5 15,246 - -0.8 15,247 + +0.2 15,247 + -0.3 15,247 + +0.7 15,253 + +0.3 15,267 + -1.1 15,279 - -3.4 15,280 + -3.4 15,515 + MMP_RS00060 0.16 -1.0 15,590 + MMP_RS00060 0.23 -1.7 16,082 + MMP_RS00060 0.69 -2.0 16,458 + -1.0 16,524 - +1.1 16,525 + -0.5 16,525 + -1.2 16,525 + +0.1 16,536 - +0.3 16,536 - -0.4 16,536 - -0.6 16,537 + +0.2 16,555 - -0.2 16,556 + +0.2 16,556 + +0.5 16,556 + +0.0 16,569 - -0.4 16,578 - +1.0 16,579 + +1.2 16,605 + -0.6 16,628 + +0.7 16,647 + +0.1 16,648 - +1.1 16,655 + +0.9 16,655 + -0.1 16,682 - +0.2 16,683 + +0.2 16,683 + -1.1 16,737 + -0.7 16,782 + -0.8 16,791 + -1.8 16,883 + -3.8 17,295 - -2.7
Or see this region's nucleotide sequence