Experiment: MoLS4 minimal media with L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0442 and DvMF_0443 are separated by 96 nucleotides DvMF_0443 and DvMF_0444 are separated by 9 nucleotides DvMF_0444 and DvMF_0445 are separated by 180 nucleotides
DvMF_0442: DvMF_0442 - Phenylacetate--CoA ligase (RefSeq), at 518,327 to 519,631
_0442
DvMF_0443: DvMF_0443 - hypothetical protein (RefSeq), at 519,728 to 520,174
_0443
DvMF_0444: DvMF_0444 - hypothetical protein (RefSeq), at 520,184 to 520,546
_0444
DvMF_0445: DvMF_0445 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 520,727 to 521,233
_0445
Position (kb) 519
520
521 Strain fitness (log2 ratio) -1
0
1 at 518.769 kb on - strand, within DvMF_0442 at 518.857 kb on + strand, within DvMF_0442 at 518.870 kb on - strand, within DvMF_0442 at 518.878 kb on - strand, within DvMF_0442 at 518.888 kb on - strand, within DvMF_0442 at 519.024 kb on + strand, within DvMF_0442 at 519.154 kb on - strand, within DvMF_0442 at 519.180 kb on + strand, within DvMF_0442 at 519.203 kb on - strand, within DvMF_0442 at 519.887 kb on - strand, within DvMF_0443 at 519.897 kb on - strand, within DvMF_0443 at 520.197 kb on - strand at 520.906 kb on + strand, within DvMF_0445 at 521.055 kb on - strand, within DvMF_0445 at 521.122 kb on - strand, within DvMF_0445
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media with L-Histidine remove 518,769 - DvMF_0442 0.34 -0.3 518,857 + DvMF_0442 0.41 -0.8 518,870 - DvMF_0442 0.42 -0.7 518,878 - DvMF_0442 0.42 -0.4 518,888 - DvMF_0442 0.43 -0.8 519,024 + DvMF_0442 0.53 +0.8 519,154 - DvMF_0442 0.63 +0.0 519,180 + DvMF_0442 0.65 -0.4 519,203 - DvMF_0442 0.67 -0.4 519,887 - DvMF_0443 0.36 -1.4 519,897 - DvMF_0443 0.38 -0.4 520,197 - -0.0 520,906 + DvMF_0445 0.35 -0.4 521,055 - DvMF_0445 0.65 -0.6 521,122 - DvMF_0445 0.78 -1.6
Or see this region's nucleotide sequence