Experiment: MoLS4 minimal media with L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0318 and DvMF_0319 are separated by 209 nucleotides DvMF_0319 and DvMF_0320 are separated by 10 nucleotides
DvMF_0318: DvMF_0318 - Exonuclease RNase T and DNA polymerase III (RefSeq), at 369,894 to 370,697
_0318
DvMF_0319: DvMF_0319 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 370,907 to 371,416
_0319
DvMF_0320: DvMF_0320 - Formate dehydrogenase (RefSeq), at 371,427 to 373,529
_0320
Position (kb) 370
371
372 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 369.972 kb on + strand at 370.002 kb on - strand, within DvMF_0318 at 370.109 kb on + strand, within DvMF_0318 at 370.109 kb on + strand, within DvMF_0318 at 370.117 kb on - strand, within DvMF_0318 at 370.117 kb on - strand, within DvMF_0318 at 370.283 kb on - strand, within DvMF_0318 at 370.288 kb on - strand, within DvMF_0318 at 370.369 kb on - strand, within DvMF_0318 at 370.376 kb on + strand, within DvMF_0318 at 370.509 kb on - strand, within DvMF_0318 at 370.513 kb on + strand, within DvMF_0318 at 370.559 kb on - strand, within DvMF_0318 at 370.637 kb on - strand at 370.796 kb on - strand at 370.899 kb on + strand at 370.956 kb on - strand at 371.266 kb on - strand, within DvMF_0319 at 371.295 kb on + strand, within DvMF_0319 at 371.344 kb on - strand, within DvMF_0319 at 371.434 kb on - strand at 371.443 kb on + strand at 371.451 kb on - strand at 371.542 kb on + strand at 371.542 kb on + strand at 371.550 kb on - strand at 371.775 kb on - strand, within DvMF_0320 at 371.779 kb on + strand, within DvMF_0320 at 371.785 kb on - strand, within DvMF_0320 at 371.862 kb on - strand, within DvMF_0320 at 371.934 kb on + strand, within DvMF_0320 at 372.016 kb on + strand, within DvMF_0320 at 372.233 kb on - strand, within DvMF_0320 at 372.376 kb on + strand, within DvMF_0320
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media with L-Histidine remove 369,972 + -1.1 370,002 - DvMF_0318 0.13 -0.6 370,109 + DvMF_0318 0.27 -0.3 370,109 + DvMF_0318 0.27 -0.4 370,117 - DvMF_0318 0.28 +0.2 370,117 - DvMF_0318 0.28 +0.0 370,283 - DvMF_0318 0.48 -0.5 370,288 - DvMF_0318 0.49 +0.7 370,369 - DvMF_0318 0.59 -0.2 370,376 + DvMF_0318 0.60 -1.0 370,509 - DvMF_0318 0.76 +1.2 370,513 + DvMF_0318 0.77 -0.4 370,559 - DvMF_0318 0.83 +0.2 370,637 - +0.3 370,796 - -1.6 370,899 + -1.5 370,956 - +0.6 371,266 - DvMF_0319 0.70 -0.8 371,295 + DvMF_0319 0.76 -0.9 371,344 - DvMF_0319 0.86 -0.9 371,434 - +0.2 371,443 + -0.3 371,451 - -0.7 371,542 + +1.6 371,542 + -2.0 371,550 - -0.0 371,775 - DvMF_0320 0.17 -1.1 371,779 + DvMF_0320 0.17 -0.1 371,785 - DvMF_0320 0.17 -1.0 371,862 - DvMF_0320 0.21 -0.0 371,934 + DvMF_0320 0.24 +1.3 372,016 + DvMF_0320 0.28 -0.1 372,233 - DvMF_0320 0.38 -3.4 372,376 + DvMF_0320 0.45 -0.7
Or see this region's nucleotide sequence