Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27460 and BWI76_RS27465 are separated by 108 nucleotides BWI76_RS27465 and BWI76_RS27470 are separated by 109 nucleotides BWI76_RS27470 and BWI76_RS27475 are separated by 98 nucleotides BWI76_RS27475 and BWI76_RS27480 are separated by 62 nucleotides
BWI76_RS27460: BWI76_RS27460 - hypothetical protein, at 5,697,293 to 5,697,892
_RS27460
BWI76_RS27465: BWI76_RS27465 - triose-phosphate isomerase, at 5,698,001 to 5,698,768
_RS27465
BWI76_RS27470: BWI76_RS27470 - sodium:sulfate symporter family protein, at 5,698,878 to 5,700,182
_RS27470
BWI76_RS27475: BWI76_RS27475 - regulatory protein GntR family protein, at 5,700,281 to 5,701,003
_RS27475
BWI76_RS27480: BWI76_RS27480 - hypothetical protein, at 5,701,066 to 5,702,106
_RS27480
Position (kb)
5698
5699
5700
5701 Strain fitness (log2 ratio)
-2
-1
0
1 at 5697.921 kb on + strand at 5697.921 kb on + strand at 5697.929 kb on + strand at 5697.941 kb on + strand at 5697.943 kb on + strand at 5698.773 kb on + strand at 5698.902 kb on - strand at 5698.954 kb on + strand at 5699.039 kb on + strand, within BWI76_RS27470 at 5699.065 kb on - strand, within BWI76_RS27470 at 5699.121 kb on + strand, within BWI76_RS27470 at 5699.127 kb on - strand, within BWI76_RS27470 at 5699.407 kb on + strand, within BWI76_RS27470 at 5699.425 kb on - strand, within BWI76_RS27470 at 5699.425 kb on - strand, within BWI76_RS27470 at 5699.566 kb on - strand, within BWI76_RS27470 at 5699.568 kb on + strand, within BWI76_RS27470 at 5699.581 kb on + strand, within BWI76_RS27470 at 5699.617 kb on + strand, within BWI76_RS27470 at 5699.853 kb on + strand, within BWI76_RS27470 at 5699.853 kb on + strand, within BWI76_RS27470 at 5699.866 kb on - strand, within BWI76_RS27470 at 5699.938 kb on - strand, within BWI76_RS27470 at 5700.093 kb on - strand at 5700.112 kb on + strand at 5700.153 kb on + strand at 5700.228 kb on + strand at 5700.279 kb on + strand at 5700.287 kb on - strand at 5700.342 kb on - strand at 5700.347 kb on + strand at 5700.424 kb on + strand, within BWI76_RS27475 at 5700.424 kb on + strand, within BWI76_RS27475 at 5700.424 kb on + strand, within BWI76_RS27475 at 5700.424 kb on + strand, within BWI76_RS27475 at 5700.424 kb on + strand, within BWI76_RS27475 at 5700.432 kb on - strand, within BWI76_RS27475 at 5700.432 kb on - strand, within BWI76_RS27475 at 5700.432 kb on - strand, within BWI76_RS27475 at 5700.432 kb on - strand, within BWI76_RS27475 at 5700.432 kb on - strand, within BWI76_RS27475 at 5700.432 kb on - strand, within BWI76_RS27475 at 5700.432 kb on - strand, within BWI76_RS27475 at 5700.432 kb on - strand, within BWI76_RS27475 at 5700.435 kb on + strand, within BWI76_RS27475 at 5700.435 kb on + strand, within BWI76_RS27475 at 5700.479 kb on + strand, within BWI76_RS27475 at 5700.514 kb on + strand, within BWI76_RS27475 at 5700.514 kb on + strand, within BWI76_RS27475 at 5700.514 kb on + strand, within BWI76_RS27475 at 5700.514 kb on + strand, within BWI76_RS27475 at 5700.522 kb on - strand, within BWI76_RS27475 at 5700.522 kb on - strand, within BWI76_RS27475 at 5700.527 kb on - strand, within BWI76_RS27475 at 5700.637 kb on + strand, within BWI76_RS27475 at 5700.645 kb on - strand, within BWI76_RS27475 at 5700.780 kb on - strand, within BWI76_RS27475 at 5700.827 kb on + strand, within BWI76_RS27475 at 5700.835 kb on - strand, within BWI76_RS27475 at 5700.837 kb on + strand, within BWI76_RS27475 at 5700.877 kb on - strand, within BWI76_RS27475 at 5700.976 kb on + strand at 5701.012 kb on + strand at 5701.012 kb on + strand at 5701.012 kb on + strand at 5701.020 kb on - strand at 5701.020 kb on - strand at 5701.020 kb on - strand at 5701.020 kb on - strand at 5701.045 kb on + strand at 5701.055 kb on + strand at 5701.089 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 5,697,921 + -0.2 5,697,921 + +1.4 5,697,929 + -0.3 5,697,941 + -1.8 5,697,943 + +0.9 5,698,773 + +0.2 5,698,902 - +1.4 5,698,954 + -0.3 5,699,039 + BWI76_RS27470 0.12 +0.1 5,699,065 - BWI76_RS27470 0.14 +0.1 5,699,121 + BWI76_RS27470 0.19 -1.1 5,699,127 - BWI76_RS27470 0.19 +0.6 5,699,407 + BWI76_RS27470 0.41 -0.1 5,699,425 - BWI76_RS27470 0.42 +0.3 5,699,425 - BWI76_RS27470 0.42 -0.3 5,699,566 - BWI76_RS27470 0.53 +0.7 5,699,568 + BWI76_RS27470 0.53 -0.0 5,699,581 + BWI76_RS27470 0.54 +0.5 5,699,617 + BWI76_RS27470 0.57 -0.2 5,699,853 + BWI76_RS27470 0.75 -0.7 5,699,853 + BWI76_RS27470 0.75 -0.6 5,699,866 - BWI76_RS27470 0.76 +0.1 5,699,938 - BWI76_RS27470 0.81 +0.1 5,700,093 - -0.6 5,700,112 + -0.4 5,700,153 + -0.1 5,700,228 + -0.1 5,700,279 + +1.2 5,700,287 - +1.5 5,700,342 - -0.5 5,700,347 + -0.8 5,700,424 + BWI76_RS27475 0.20 -1.3 5,700,424 + BWI76_RS27475 0.20 -0.2 5,700,424 + BWI76_RS27475 0.20 +0.2 5,700,424 + BWI76_RS27475 0.20 -0.3 5,700,424 + BWI76_RS27475 0.20 -1.8 5,700,432 - BWI76_RS27475 0.21 -0.6 5,700,432 - BWI76_RS27475 0.21 -0.5 5,700,432 - BWI76_RS27475 0.21 -0.6 5,700,432 - BWI76_RS27475 0.21 -0.6 5,700,432 - BWI76_RS27475 0.21 -0.8 5,700,432 - BWI76_RS27475 0.21 -0.4 5,700,432 - BWI76_RS27475 0.21 -0.7 5,700,432 - BWI76_RS27475 0.21 -1.5 5,700,435 + BWI76_RS27475 0.21 -0.6 5,700,435 + BWI76_RS27475 0.21 -0.5 5,700,479 + BWI76_RS27475 0.27 -1.7 5,700,514 + BWI76_RS27475 0.32 -0.1 5,700,514 + BWI76_RS27475 0.32 -0.9 5,700,514 + BWI76_RS27475 0.32 -1.0 5,700,514 + BWI76_RS27475 0.32 +0.6 5,700,522 - BWI76_RS27475 0.33 -0.7 5,700,522 - BWI76_RS27475 0.33 -2.0 5,700,527 - BWI76_RS27475 0.34 -0.4 5,700,637 + BWI76_RS27475 0.49 -0.4 5,700,645 - BWI76_RS27475 0.50 -0.1 5,700,780 - BWI76_RS27475 0.69 +1.0 5,700,827 + BWI76_RS27475 0.76 +0.1 5,700,835 - BWI76_RS27475 0.77 -1.4 5,700,837 + BWI76_RS27475 0.77 +0.2 5,700,877 - BWI76_RS27475 0.82 -1.3 5,700,976 + -0.1 5,701,012 + -1.1 5,701,012 + +0.9 5,701,012 + +0.6 5,701,020 - -0.5 5,701,020 - +0.2 5,701,020 - -0.4 5,701,020 - +0.4 5,701,045 + +0.2 5,701,055 + -0.2 5,701,089 + -1.3
Or see this region's nucleotide sequence