Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26085 and BWI76_RS26090 are separated by 54 nucleotides BWI76_RS26090 and BWI76_RS26095 are separated by 19 nucleotides BWI76_RS26095 and BWI76_RS26100 are separated by 10 nucleotides
BWI76_RS26085: BWI76_RS26085 - 3-dehydroquinate synthase, at 5,390,178 to 5,391,272
_RS26085
BWI76_RS26090: BWI76_RS26090 - shikimate kinase, at 5,391,327 to 5,391,848
_RS26090
BWI76_RS26095: BWI76_RS26095 - shikimate kinase, at 5,391,868 to 5,392,106
_RS26095
BWI76_RS26100: BWI76_RS26100 - DNA transporter HofQ, at 5,392,117 to 5,393,358
_RS26100
Position (kb) 5391
5392
5393 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5391.051 kb on - strand, within BWI76_RS26085 at 5391.138 kb on - strand, within BWI76_RS26085 at 5391.913 kb on - strand, within BWI76_RS26095 at 5391.929 kb on - strand, within BWI76_RS26095 at 5392.235 kb on - strand at 5392.260 kb on + strand, within BWI76_RS26100 at 5392.287 kb on - strand, within BWI76_RS26100 at 5392.447 kb on + strand, within BWI76_RS26100 at 5392.460 kb on + strand, within BWI76_RS26100 at 5392.696 kb on + strand, within BWI76_RS26100 at 5392.704 kb on - strand, within BWI76_RS26100 at 5393.007 kb on - strand, within BWI76_RS26100 at 5393.091 kb on + strand, within BWI76_RS26100 at 5393.099 kb on - strand, within BWI76_RS26100
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 5,391,051 - BWI76_RS26085 0.80 -0.5 5,391,138 - BWI76_RS26085 0.88 -3.1 5,391,913 - BWI76_RS26095 0.19 -1.7 5,391,929 - BWI76_RS26095 0.26 -1.4 5,392,235 - +0.3 5,392,260 + BWI76_RS26100 0.12 -0.5 5,392,287 - BWI76_RS26100 0.14 +1.2 5,392,447 + BWI76_RS26100 0.27 -0.5 5,392,460 + BWI76_RS26100 0.28 -0.9 5,392,696 + BWI76_RS26100 0.47 +0.4 5,392,704 - BWI76_RS26100 0.47 -0.2 5,393,007 - BWI76_RS26100 0.72 +0.1 5,393,091 + BWI76_RS26100 0.78 -0.5 5,393,099 - BWI76_RS26100 0.79 +0.7
Or see this region's nucleotide sequence