Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24310 and BWI76_RS24315 are separated by 120 nucleotides BWI76_RS24315 and BWI76_RS24320 are separated by 84 nucleotides
BWI76_RS24310: BWI76_RS24310 - YgiQ family radical SAM protein, at 5,064,647 to 5,066,830
_RS24310
BWI76_RS24315: BWI76_RS24315 - cell division protein FtsP, at 5,066,951 to 5,068,363
_RS24315
BWI76_RS24320: BWI76_RS24320 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 5,068,448 to 5,069,185
_RS24320
Position (kb)
5066
5067
5068
5069 Strain fitness (log2 ratio)
-1
0
1 at 5065.954 kb on + strand, within BWI76_RS24310 at 5066.077 kb on + strand, within BWI76_RS24310 at 5066.173 kb on - strand, within BWI76_RS24310 at 5066.400 kb on - strand, within BWI76_RS24310 at 5066.405 kb on - strand, within BWI76_RS24310 at 5066.479 kb on - strand, within BWI76_RS24310 at 5066.546 kb on + strand, within BWI76_RS24310 at 5066.561 kb on - strand, within BWI76_RS24310 at 5066.924 kb on + strand at 5066.939 kb on + strand at 5066.939 kb on + strand at 5066.985 kb on - strand at 5067.060 kb on - strand at 5067.255 kb on + strand, within BWI76_RS24315 at 5067.263 kb on - strand at 5067.554 kb on - strand, within BWI76_RS24315 at 5067.656 kb on - strand, within BWI76_RS24315 at 5067.767 kb on + strand, within BWI76_RS24315 at 5067.775 kb on - strand, within BWI76_RS24315 at 5067.893 kb on - strand, within BWI76_RS24315 at 5068.058 kb on - strand, within BWI76_RS24315 at 5068.135 kb on + strand, within BWI76_RS24315 at 5068.279 kb on - strand at 5068.326 kb on - strand at 5069.213 kb on + strand at 5069.221 kb on - strand at 5069.227 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 5,065,954 + BWI76_RS24310 0.60 -0.3 5,066,077 + BWI76_RS24310 0.65 +0.5 5,066,173 - BWI76_RS24310 0.70 +0.0 5,066,400 - BWI76_RS24310 0.80 -0.4 5,066,405 - BWI76_RS24310 0.80 +0.8 5,066,479 - BWI76_RS24310 0.84 +0.4 5,066,546 + BWI76_RS24310 0.87 +0.1 5,066,561 - BWI76_RS24310 0.88 -0.0 5,066,924 + -1.2 5,066,939 + -0.3 5,066,939 + -0.3 5,066,985 - -0.4 5,067,060 - +0.2 5,067,255 + BWI76_RS24315 0.22 -0.8 5,067,263 - -1.2 5,067,554 - BWI76_RS24315 0.43 -1.4 5,067,656 - BWI76_RS24315 0.50 +0.5 5,067,767 + BWI76_RS24315 0.58 -0.5 5,067,775 - BWI76_RS24315 0.58 -1.1 5,067,893 - BWI76_RS24315 0.67 -0.6 5,068,058 - BWI76_RS24315 0.78 -0.7 5,068,135 + BWI76_RS24315 0.84 -0.4 5,068,279 - -1.2 5,068,326 - -0.6 5,069,213 + -0.1 5,069,221 - -0.6 5,069,227 + -0.5
Or see this region's nucleotide sequence