Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11685 and BWI76_RS11690 are separated by 12 nucleotides BWI76_RS11690 and BWI76_RS11695 are separated by 349 nucleotides
BWI76_RS11685: BWI76_RS11685 - Succinylarginine dihydrolase (EC 3.5.3.23) (from data) , at 2,458,584 to 2,459,909
_RS11685
BWI76_RS11690: BWI76_RS11690 - succinylglutamate desuccinylase, at 2,459,922 to 2,460,887
_RS11690
BWI76_RS11695: BWI76_RS11695 - ATP-independent periplasmic protein-refolding chaperone, at 2,461,237 to 2,461,719
_RS11695
Position (kb)
2459
2460
2461 Strain fitness (log2 ratio)
-2
-1
0
1 at 2459.086 kb on - strand, within BWI76_RS11685 at 2459.212 kb on - strand, within BWI76_RS11685 at 2459.495 kb on + strand, within BWI76_RS11685 at 2459.605 kb on + strand, within BWI76_RS11685 at 2459.632 kb on + strand, within BWI76_RS11685 at 2459.761 kb on + strand, within BWI76_RS11685 at 2459.791 kb on - strand at 2459.868 kb on - strand at 2460.004 kb on + strand at 2460.154 kb on + strand, within BWI76_RS11690 at 2460.533 kb on + strand, within BWI76_RS11690 at 2460.706 kb on + strand, within BWI76_RS11690 at 2460.739 kb on + strand, within BWI76_RS11690 at 2460.747 kb on - strand, within BWI76_RS11690 at 2460.861 kb on + strand at 2460.861 kb on + strand at 2460.862 kb on + strand at 2460.869 kb on - strand at 2460.870 kb on + strand at 2460.870 kb on - strand at 2460.872 kb on + strand at 2460.878 kb on - strand at 2460.878 kb on - strand at 2460.878 kb on - strand at 2460.886 kb on - strand at 2461.161 kb on + strand at 2461.161 kb on - strand at 2461.182 kb on + strand at 2461.195 kb on + strand at 2461.195 kb on - strand at 2461.252 kb on + strand at 2461.257 kb on + strand at 2461.265 kb on + strand at 2461.270 kb on + strand at 2461.273 kb on - strand at 2461.281 kb on + strand at 2461.286 kb on + strand, within BWI76_RS11695 at 2461.294 kb on - strand, within BWI76_RS11695 at 2461.294 kb on - strand, within BWI76_RS11695 at 2461.316 kb on + strand, within BWI76_RS11695 at 2461.324 kb on - strand, within BWI76_RS11695 at 2461.386 kb on + strand, within BWI76_RS11695 at 2461.394 kb on - strand, within BWI76_RS11695 at 2461.479 kb on - strand, within BWI76_RS11695 at 2461.480 kb on - strand, within BWI76_RS11695 at 2461.535 kb on - strand, within BWI76_RS11695 at 2461.554 kb on + strand, within BWI76_RS11695 at 2461.579 kb on + strand, within BWI76_RS11695 at 2461.714 kb on + strand at 2461.714 kb on + strand at 2461.731 kb on + strand at 2461.777 kb on - strand at 2461.777 kb on - strand at 2461.777 kb on - strand at 2461.777 kb on - strand at 2461.857 kb on - strand at 2461.869 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 2,459,086 - BWI76_RS11685 0.38 -0.3 2,459,212 - BWI76_RS11685 0.47 +0.7 2,459,495 + BWI76_RS11685 0.69 -0.1 2,459,605 + BWI76_RS11685 0.77 -0.3 2,459,632 + BWI76_RS11685 0.79 -0.5 2,459,761 + BWI76_RS11685 0.89 -0.2 2,459,791 - -0.2 2,459,868 - -0.6 2,460,004 + -0.4 2,460,154 + BWI76_RS11690 0.24 -1.6 2,460,533 + BWI76_RS11690 0.63 -0.7 2,460,706 + BWI76_RS11690 0.81 -1.3 2,460,739 + BWI76_RS11690 0.85 -0.6 2,460,747 - BWI76_RS11690 0.85 -0.9 2,460,861 + -0.6 2,460,861 + -0.8 2,460,862 + -0.5 2,460,869 - -0.4 2,460,870 + -0.5 2,460,870 - -0.4 2,460,872 + -0.6 2,460,878 - -2.0 2,460,878 - -1.2 2,460,878 - +0.5 2,460,886 - -0.3 2,461,161 + +0.1 2,461,161 - +0.4 2,461,182 + +0.4 2,461,195 + +0.5 2,461,195 - -0.6 2,461,252 + -0.1 2,461,257 + -0.7 2,461,265 + -0.1 2,461,270 + -1.7 2,461,273 - +0.2 2,461,281 + -0.0 2,461,286 + BWI76_RS11695 0.10 +0.8 2,461,294 - BWI76_RS11695 0.12 +0.7 2,461,294 - BWI76_RS11695 0.12 -0.9 2,461,316 + BWI76_RS11695 0.16 -1.1 2,461,324 - BWI76_RS11695 0.18 +0.4 2,461,386 + BWI76_RS11695 0.31 -0.1 2,461,394 - BWI76_RS11695 0.33 +0.5 2,461,479 - BWI76_RS11695 0.50 +0.3 2,461,480 - BWI76_RS11695 0.50 +0.6 2,461,535 - BWI76_RS11695 0.62 +0.0 2,461,554 + BWI76_RS11695 0.66 -0.2 2,461,579 + BWI76_RS11695 0.71 -0.1 2,461,714 + +0.5 2,461,714 + -0.4 2,461,731 + -0.3 2,461,777 - -0.5 2,461,777 - -1.2 2,461,777 - +0.6 2,461,777 - -0.4 2,461,857 - +0.2 2,461,869 - +0.2
Or see this region's nucleotide sequence