Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08530 and BWI76_RS08535 are separated by 128 nucleotides BWI76_RS08535 and BWI76_RS08540 are separated by 34 nucleotides BWI76_RS08540 and BWI76_RS08545 are separated by 9 nucleotides
BWI76_RS08530: BWI76_RS08530 - protein TolA, at 1,805,018 to 1,806,355
_RS08530
BWI76_RS08535: BWI76_RS08535 - translocation protein TolB, at 1,806,484 to 1,807,776
_RS08535
BWI76_RS08540: BWI76_RS08540 - hypothetical protein, at 1,807,811 to 1,808,335
_RS08540
BWI76_RS08545: BWI76_RS08545 - cell division protein CpoB, at 1,808,345 to 1,809,130
_RS08545
Position (kb) 1806
1807
1808 Strain fitness (log2 ratio) -2
-1
0
1 at 1806.168 kb on - strand, within BWI76_RS08530 at 1806.468 kb on + strand at 1806.476 kb on - strand at 1806.619 kb on + strand, within BWI76_RS08535 at 1806.763 kb on - strand at 1807.007 kb on - strand, within BWI76_RS08535 at 1807.291 kb on - strand, within BWI76_RS08535 at 1807.741 kb on - strand at 1807.756 kb on + strand at 1808.154 kb on - strand, within BWI76_RS08540 at 1808.166 kb on + strand, within BWI76_RS08540
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 1,806,168 - BWI76_RS08530 0.86 -1.5 1,806,468 + +0.4 1,806,476 - -0.3 1,806,619 + BWI76_RS08535 0.10 -1.4 1,806,763 - +0.1 1,807,007 - BWI76_RS08535 0.40 -1.9 1,807,291 - BWI76_RS08535 0.62 +1.4 1,807,741 - +0.1 1,807,756 + -0.2 1,808,154 - BWI76_RS08540 0.65 -1.3 1,808,166 + BWI76_RS08540 0.68 -2.1
Or see this region's nucleotide sequence