Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05525 and BWI76_RS05530 are separated by 159 nucleotides BWI76_RS05530 and BWI76_RS05535 are separated by 2 nucleotides BWI76_RS05535 and BWI76_RS05540 are separated by 111 nucleotides
BWI76_RS05525: BWI76_RS05525 - glycerophosphodiester phosphodiesterase, at 1,157,926 to 1,158,783
_RS05525
BWI76_RS05530: BWI76_RS05530 - DNA polymerase IV, at 1,158,943 to 1,159,998
_RS05530
BWI76_RS05535: BWI76_RS05535 - acyltransferase, at 1,160,001 to 1,160,492
_RS05535
BWI76_RS05540: BWI76_RS05540 - cytosol nonspecific dipeptidase, at 1,160,604 to 1,162,061
_RS05540
Position (kb)
1158
1159
1160 Strain fitness (log2 ratio)
-2
-1
0
1 at 1158.058 kb on + strand, within BWI76_RS05525 at 1158.058 kb on + strand, within BWI76_RS05525 at 1158.329 kb on - strand, within BWI76_RS05525 at 1158.330 kb on - strand, within BWI76_RS05525 at 1158.542 kb on - strand, within BWI76_RS05525 at 1158.945 kb on + strand at 1158.964 kb on + strand at 1158.964 kb on + strand at 1159.004 kb on + strand at 1159.004 kb on + strand at 1159.004 kb on + strand at 1159.051 kb on - strand, within BWI76_RS05530 at 1159.164 kb on - strand, within BWI76_RS05530 at 1159.410 kb on - strand, within BWI76_RS05530 at 1159.482 kb on + strand, within BWI76_RS05530 at 1159.523 kb on - strand, within BWI76_RS05530 at 1159.528 kb on + strand, within BWI76_RS05530 at 1159.549 kb on + strand, within BWI76_RS05530 at 1159.886 kb on - strand, within BWI76_RS05530 at 1159.944 kb on + strand at 1159.969 kb on - strand at 1159.984 kb on + strand at 1159.991 kb on + strand at 1160.492 kb on + strand at 1160.586 kb on + strand at 1160.594 kb on - strand at 1160.644 kb on + strand at 1160.649 kb on + strand at 1160.649 kb on + strand at 1160.652 kb on - strand at 1160.715 kb on + strand at 1160.763 kb on + strand, within BWI76_RS05540 at 1160.763 kb on + strand, within BWI76_RS05540 at 1160.763 kb on + strand, within BWI76_RS05540 at 1160.810 kb on - strand, within BWI76_RS05540
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 1,158,058 + BWI76_RS05525 0.15 -0.7 1,158,058 + BWI76_RS05525 0.15 -0.8 1,158,329 - BWI76_RS05525 0.47 -0.5 1,158,330 - BWI76_RS05525 0.47 -0.4 1,158,542 - BWI76_RS05525 0.72 -0.8 1,158,945 + -0.4 1,158,964 + +0.3 1,158,964 + -0.5 1,159,004 + -2.2 1,159,004 + -0.0 1,159,004 + -0.8 1,159,051 - BWI76_RS05530 0.10 -0.2 1,159,164 - BWI76_RS05530 0.21 +0.0 1,159,410 - BWI76_RS05530 0.44 -0.1 1,159,482 + BWI76_RS05530 0.51 +0.2 1,159,523 - BWI76_RS05530 0.55 +0.1 1,159,528 + BWI76_RS05530 0.55 +0.1 1,159,549 + BWI76_RS05530 0.57 +0.6 1,159,886 - BWI76_RS05530 0.89 -0.7 1,159,944 + +0.2 1,159,969 - -1.4 1,159,984 + +0.1 1,159,991 + -0.4 1,160,492 + -0.4 1,160,586 + -0.0 1,160,594 - -0.6 1,160,644 + -1.2 1,160,649 + +0.6 1,160,649 + -0.7 1,160,652 - -0.2 1,160,715 + +1.3 1,160,763 + BWI76_RS05540 0.11 -0.5 1,160,763 + BWI76_RS05540 0.11 -1.3 1,160,763 + BWI76_RS05540 0.11 -0.1 1,160,810 - BWI76_RS05540 0.14 +0.1
Or see this region's nucleotide sequence