Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02435 and BWI76_RS02440 are separated by 140 nucleotides BWI76_RS02440 and BWI76_RS02445 are separated by 10 nucleotides BWI76_RS02445 and BWI76_RS02450 are separated by 10 nucleotides BWI76_RS02450 and BWI76_RS02455 overlap by 8 nucleotides
BWI76_RS02435: BWI76_RS02435 - outer membrane lipoprotein Blc, at 499,493 to 500,023
_RS02435
BWI76_RS02440: BWI76_RS02440 - fumarate reductase subunit D, at 500,164 to 500,523
_RS02440
BWI76_RS02445: BWI76_RS02445 - fumarate reductase subunit C, at 500,534 to 500,929
_RS02445
BWI76_RS02450: BWI76_RS02450 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 500,940 to 501,674
_RS02450
BWI76_RS02455: BWI76_RS02455 - fumarate reductase flavoprotein subunit, at 501,667 to 503,457
_RS02455
Position (kb) 500
501
502 Strain fitness (log2 ratio) -2
-1
0
1 at 499.960 kb on + strand, within BWI76_RS02435 at 499.960 kb on + strand, within BWI76_RS02435 at 499.960 kb on + strand, within BWI76_RS02435 at 500.052 kb on + strand at 500.299 kb on - strand, within BWI76_RS02440 at 500.304 kb on + strand, within BWI76_RS02440 at 500.463 kb on - strand, within BWI76_RS02440 at 500.543 kb on - strand at 500.543 kb on - strand at 500.586 kb on + strand, within BWI76_RS02445 at 500.762 kb on + strand, within BWI76_RS02445 at 500.848 kb on + strand, within BWI76_RS02445 at 500.850 kb on + strand, within BWI76_RS02445 at 500.911 kb on - strand at 500.948 kb on + strand at 500.956 kb on - strand at 500.994 kb on - strand at 501.287 kb on + strand, within BWI76_RS02450 at 501.420 kb on - strand, within BWI76_RS02450 at 501.475 kb on - strand, within BWI76_RS02450 at 501.505 kb on + strand, within BWI76_RS02450 at 501.552 kb on - strand, within BWI76_RS02450 at 501.601 kb on + strand at 501.601 kb on + strand at 501.688 kb on + strand at 501.874 kb on - strand, within BWI76_RS02455 at 501.941 kb on + strand, within BWI76_RS02455 at 501.941 kb on + strand, within BWI76_RS02455 at 501.949 kb on - strand, within BWI76_RS02455 at 501.949 kb on - strand, within BWI76_RS02455 at 501.964 kb on - strand, within BWI76_RS02455 at 501.974 kb on - strand, within BWI76_RS02455 at 502.086 kb on + strand, within BWI76_RS02455 at 502.122 kb on - strand, within BWI76_RS02455 at 502.305 kb on - strand, within BWI76_RS02455 at 502.381 kb on + strand, within BWI76_RS02455 at 502.439 kb on + strand, within BWI76_RS02455 at 502.483 kb on - strand, within BWI76_RS02455 at 502.493 kb on - strand, within BWI76_RS02455 at 502.529 kb on - strand, within BWI76_RS02455 at 502.552 kb on + strand, within BWI76_RS02455 at 502.553 kb on + strand, within BWI76_RS02455 at 502.577 kb on + strand, within BWI76_RS02455 at 502.607 kb on - strand, within BWI76_RS02455
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 499,960 + BWI76_RS02435 0.88 -0.4 499,960 + BWI76_RS02435 0.88 +0.3 499,960 + BWI76_RS02435 0.88 +0.1 500,052 + +0.6 500,299 - BWI76_RS02440 0.38 -0.0 500,304 + BWI76_RS02440 0.39 +1.4 500,463 - BWI76_RS02440 0.83 -0.3 500,543 - +0.9 500,543 - -0.2 500,586 + BWI76_RS02445 0.13 +1.5 500,762 + BWI76_RS02445 0.58 +0.1 500,848 + BWI76_RS02445 0.79 -0.4 500,850 + BWI76_RS02445 0.80 -0.1 500,911 - -0.4 500,948 + +0.6 500,956 - -0.6 500,994 - -0.3 501,287 + BWI76_RS02450 0.47 -0.0 501,420 - BWI76_RS02450 0.65 -0.1 501,475 - BWI76_RS02450 0.73 +0.0 501,505 + BWI76_RS02450 0.77 +0.4 501,552 - BWI76_RS02450 0.83 -0.4 501,601 + -0.6 501,601 + -0.6 501,688 + -0.3 501,874 - BWI76_RS02455 0.12 -0.2 501,941 + BWI76_RS02455 0.15 +0.3 501,941 + BWI76_RS02455 0.15 -0.4 501,949 - BWI76_RS02455 0.16 -1.4 501,949 - BWI76_RS02455 0.16 -0.6 501,964 - BWI76_RS02455 0.17 -0.2 501,974 - BWI76_RS02455 0.17 -1.2 502,086 + BWI76_RS02455 0.23 +0.1 502,122 - BWI76_RS02455 0.25 +0.0 502,305 - BWI76_RS02455 0.36 +0.1 502,381 + BWI76_RS02455 0.40 -1.3 502,439 + BWI76_RS02455 0.43 +0.9 502,483 - BWI76_RS02455 0.46 -1.9 502,493 - BWI76_RS02455 0.46 -0.1 502,529 - BWI76_RS02455 0.48 +0.4 502,552 + BWI76_RS02455 0.49 +0.1 502,553 + BWI76_RS02455 0.49 -2.0 502,577 + BWI76_RS02455 0.51 +0.1 502,607 - BWI76_RS02455 0.52 -0.3
Or see this region's nucleotide sequence