Experiment: Dilute LB (1/20)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02085 and BWI76_RS02090 overlap by 4 nucleotides BWI76_RS02090 and BWI76_RS02095 are separated by 182 nucleotides BWI76_RS02095 and BWI76_RS02100 are separated by 92 nucleotides BWI76_RS02100 and BWI76_RS02105 are separated by 67 nucleotides
BWI76_RS02085: BWI76_RS02085 - cation acetate symporter, at 429,691 to 431,340
_RS02085
BWI76_RS02090: BWI76_RS02090 - membrane protein, at 431,337 to 431,651
_RS02090
BWI76_RS02095: BWI76_RS02095 - acetyl-coenzyme A synthetase, at 431,834 to 433,792
_RS02095
BWI76_RS02100: BWI76_RS02100 - hypothetical protein, at 433,885 to 434,136
_RS02100
BWI76_RS02105: BWI76_RS02105 - glutamate/aspartate:proton symporter GltP, at 434,204 to 435,517
_RS02105
Position (kb) 431
432
433
434 Strain fitness (log2 ratio) -2
-1
0
1 at 430.888 kb on + strand, within BWI76_RS02085 at 430.914 kb on + strand, within BWI76_RS02085 at 430.922 kb on - strand, within BWI76_RS02085 at 430.976 kb on - strand, within BWI76_RS02085 at 431.056 kb on - strand, within BWI76_RS02085 at 431.056 kb on - strand, within BWI76_RS02085 at 431.082 kb on - strand, within BWI76_RS02085 at 431.192 kb on + strand at 431.200 kb on - strand at 431.265 kb on - strand at 431.265 kb on - strand at 431.270 kb on - strand at 431.323 kb on + strand at 431.344 kb on + strand at 431.502 kb on - strand, within BWI76_RS02090 at 431.536 kb on - strand, within BWI76_RS02090 at 431.559 kb on - strand, within BWI76_RS02090 at 431.668 kb on - strand at 431.726 kb on - strand at 431.728 kb on + strand at 431.762 kb on + strand at 431.770 kb on - strand at 431.815 kb on + strand at 431.847 kb on + strand at 431.901 kb on + strand at 432.063 kb on + strand, within BWI76_RS02095 at 432.066 kb on - strand at 432.071 kb on - strand, within BWI76_RS02095 at 432.094 kb on + strand, within BWI76_RS02095 at 432.094 kb on + strand, within BWI76_RS02095 at 432.110 kb on + strand, within BWI76_RS02095 at 432.126 kb on + strand, within BWI76_RS02095 at 432.297 kb on + strand, within BWI76_RS02095 at 432.381 kb on + strand, within BWI76_RS02095 at 432.457 kb on + strand, within BWI76_RS02095 at 432.526 kb on + strand, within BWI76_RS02095 at 432.620 kb on - strand, within BWI76_RS02095 at 432.715 kb on + strand, within BWI76_RS02095 at 432.788 kb on + strand, within BWI76_RS02095 at 432.975 kb on + strand, within BWI76_RS02095 at 432.983 kb on - strand, within BWI76_RS02095 at 433.039 kb on + strand, within BWI76_RS02095 at 433.080 kb on + strand, within BWI76_RS02095 at 433.138 kb on - strand, within BWI76_RS02095 at 433.182 kb on + strand, within BWI76_RS02095 at 433.413 kb on + strand, within BWI76_RS02095 at 433.513 kb on - strand, within BWI76_RS02095 at 433.716 kb on + strand at 433.726 kb on + strand at 434.147 kb on + strand at 434.196 kb on + strand at 434.258 kb on - strand at 434.275 kb on - strand at 434.316 kb on + strand at 434.353 kb on - strand, within BWI76_RS02105 at 434.387 kb on + strand, within BWI76_RS02105 at 434.481 kb on + strand, within BWI76_RS02105 at 434.484 kb on - strand, within BWI76_RS02105 at 434.502 kb on + strand, within BWI76_RS02105 at 434.510 kb on - strand, within BWI76_RS02105 at 434.600 kb on + strand, within BWI76_RS02105 at 434.608 kb on - strand, within BWI76_RS02105
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/20) remove 430,888 + BWI76_RS02085 0.73 -0.2 430,914 + BWI76_RS02085 0.74 +0.4 430,922 - BWI76_RS02085 0.75 +0.1 430,976 - BWI76_RS02085 0.78 +0.4 431,056 - BWI76_RS02085 0.83 +1.3 431,056 - BWI76_RS02085 0.83 +0.1 431,082 - BWI76_RS02085 0.84 +0.0 431,192 + -0.1 431,200 - -0.4 431,265 - +0.2 431,265 - -0.1 431,270 - +0.2 431,323 + -0.4 431,344 + +0.4 431,502 - BWI76_RS02090 0.52 -0.3 431,536 - BWI76_RS02090 0.63 -0.2 431,559 - BWI76_RS02090 0.70 +0.2 431,668 - +0.5 431,726 - +1.3 431,728 + +0.6 431,762 + -0.2 431,770 - +0.0 431,815 + +0.4 431,847 + -0.5 431,901 + -0.8 432,063 + BWI76_RS02095 0.12 -1.6 432,066 - +1.4 432,071 - BWI76_RS02095 0.12 -0.5 432,094 + BWI76_RS02095 0.13 -0.2 432,094 + BWI76_RS02095 0.13 -0.9 432,110 + BWI76_RS02095 0.14 -0.8 432,126 + BWI76_RS02095 0.15 -0.7 432,297 + BWI76_RS02095 0.24 -1.4 432,381 + BWI76_RS02095 0.28 -0.5 432,457 + BWI76_RS02095 0.32 -0.8 432,526 + BWI76_RS02095 0.35 -1.8 432,620 - BWI76_RS02095 0.40 -1.8 432,715 + BWI76_RS02095 0.45 -0.2 432,788 + BWI76_RS02095 0.49 -1.3 432,975 + BWI76_RS02095 0.58 -2.0 432,983 - BWI76_RS02095 0.59 -0.7 433,039 + BWI76_RS02095 0.62 -0.3 433,080 + BWI76_RS02095 0.64 -0.2 433,138 - BWI76_RS02095 0.67 -1.2 433,182 + BWI76_RS02095 0.69 -0.2 433,413 + BWI76_RS02095 0.81 -0.5 433,513 - BWI76_RS02095 0.86 +0.2 433,716 + -2.2 433,726 + -1.1 434,147 + -0.5 434,196 + -0.4 434,258 - -0.0 434,275 - -1.0 434,316 + -0.3 434,353 - BWI76_RS02105 0.11 -0.2 434,387 + BWI76_RS02105 0.14 -0.9 434,481 + BWI76_RS02105 0.21 -0.5 434,484 - BWI76_RS02105 0.21 -2.5 434,502 + BWI76_RS02105 0.23 -1.0 434,510 - BWI76_RS02105 0.23 +0.0 434,600 + BWI76_RS02105 0.30 +1.0 434,608 - BWI76_RS02105 0.31 +0.2
Or see this region's nucleotide sequence