Experiment: MoLS4 minimal media with D,L-Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2220 and DvMF_2221 are separated by 178 nucleotides DvMF_2221 and DvMF_2222 overlap by 4 nucleotides DvMF_2222 and DvMF_2223 are separated by 263 nucleotides DvMF_2223 and DvMF_R0061 are separated by 112 nucleotides DvMF_R0061 and DvMF_2224 are separated by 97 nucleotides
DvMF_2220: DvMF_2220 - lipolytic protein G-D-S-L family (RefSeq), at 2,779,909 to 2,780,553
_2220
DvMF_2221: DvMF_2221 - ABC transporter related (RefSeq), at 2,780,732 to 2,781,490
_2221
DvMF_2222: DvMF_2222 - protein of unknown function DUF214 (RefSeq), at 2,781,487 to 2,784,294
_2222
DvMF_2223: DvMF_2223 - diacylglycerol kinase (RefSeq), at 2,784,558 to 2,784,920
_2223
DvMF_R0061: DvMF_R0061 - tRNA-Lys (RefSeq), at 2,785,033 to 2,785,108
_R0061
DvMF_2224: DvMF_2224 - seryl-tRNA synthetase (RefSeq), at 2,785,206 to 2,786,483
_2224
Position (kb)
2781
2782
2783
2784
2785 Strain fitness (log2 ratio)
-1
0
1 at 2780.620 kb on - strand at 2780.803 kb on + strand at 2780.886 kb on + strand, within DvMF_2221 at 2781.040 kb on + strand, within DvMF_2221 at 2781.050 kb on - strand, within DvMF_2221 at 2781.060 kb on + strand, within DvMF_2221 at 2781.806 kb on + strand, within DvMF_2222 at 2781.818 kb on + strand, within DvMF_2222 at 2781.875 kb on + strand, within DvMF_2222 at 2781.951 kb on + strand, within DvMF_2222 at 2781.951 kb on + strand, within DvMF_2222 at 2782.204 kb on - strand, within DvMF_2222 at 2782.534 kb on + strand, within DvMF_2222 at 2782.553 kb on - strand, within DvMF_2222 at 2782.607 kb on - strand, within DvMF_2222 at 2783.174 kb on - strand, within DvMF_2222 at 2783.461 kb on + strand, within DvMF_2222 at 2783.641 kb on + strand, within DvMF_2222 at 2783.747 kb on - strand, within DvMF_2222 at 2783.760 kb on - strand, within DvMF_2222 at 2783.844 kb on + strand, within DvMF_2222 at 2783.887 kb on - strand, within DvMF_2222 at 2784.035 kb on - strand at 2784.102 kb on + strand at 2784.222 kb on - strand at 2784.369 kb on - strand at 2784.503 kb on + strand at 2784.555 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media with D,L-Cysteine remove 2,780,620 - -0.2 2,780,803 + -0.3 2,780,886 + DvMF_2221 0.20 -0.1 2,781,040 + DvMF_2221 0.41 -0.9 2,781,050 - DvMF_2221 0.42 -0.2 2,781,060 + DvMF_2221 0.43 -0.2 2,781,806 + DvMF_2222 0.11 -0.5 2,781,818 + DvMF_2222 0.12 -0.7 2,781,875 + DvMF_2222 0.14 -0.5 2,781,951 + DvMF_2222 0.17 -0.2 2,781,951 + DvMF_2222 0.17 -0.0 2,782,204 - DvMF_2222 0.26 -0.9 2,782,534 + DvMF_2222 0.37 +0.6 2,782,553 - DvMF_2222 0.38 -0.5 2,782,607 - DvMF_2222 0.40 -1.2 2,783,174 - DvMF_2222 0.60 -0.5 2,783,461 + DvMF_2222 0.70 -0.4 2,783,641 + DvMF_2222 0.77 +0.3 2,783,747 - DvMF_2222 0.80 -0.1 2,783,760 - DvMF_2222 0.81 -0.5 2,783,844 + DvMF_2222 0.84 +0.2 2,783,887 - DvMF_2222 0.85 -0.3 2,784,035 - -1.4 2,784,102 + -1.0 2,784,222 - +0.1 2,784,369 - -0.7 2,784,503 + -0.6 2,784,555 + -0.7
Or see this region's nucleotide sequence