Experiment: MoLS4 minimal media with D,L-Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1811 and DvMF_1812 are separated by 268 nucleotides DvMF_1812 and DvMF_1813 are separated by 388 nucleotides DvMF_1813 and DvMF_1814 are separated by 101 nucleotides DvMF_1814 and DvMF_1815 are separated by 403 nucleotides
DvMF_1811: DvMF_1811 - pantothenate kinase (RefSeq), at 2,213,449 to 2,214,237
_1811
DvMF_1812: DvMF_1812 - hypothetical protein (RefSeq), at 2,214,506 to 2,214,787
_1812
DvMF_1813: DvMF_1813 - hypothetical protein (RefSeq), at 2,215,176 to 2,215,808
_1813
DvMF_1814: DvMF_1814 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 2,215,910 to 2,216,104
_1814
DvMF_1815: DvMF_1815 - hypothetical protein (RefSeq), at 2,216,508 to 2,216,786
_1815
Position (kb)
2215
2216 Strain fitness (log2 ratio)
-1
0
1 at 2214.271 kb on + strand at 2214.278 kb on + strand at 2214.279 kb on - strand at 2214.294 kb on - strand at 2214.311 kb on - strand at 2214.420 kb on + strand at 2214.793 kb on + strand at 2214.920 kb on - strand at 2214.930 kb on - strand at 2215.092 kb on - strand at 2215.214 kb on - strand at 2215.236 kb on - strand at 2215.283 kb on - strand, within DvMF_1813 at 2215.315 kb on + strand, within DvMF_1813 at 2215.371 kb on + strand, within DvMF_1813 at 2215.628 kb on - strand, within DvMF_1813 at 2215.630 kb on + strand, within DvMF_1813 at 2215.832 kb on - strand at 2215.902 kb on - strand at 2216.090 kb on - strand at 2216.156 kb on - strand at 2216.184 kb on - strand at 2216.309 kb on - strand at 2216.507 kb on + strand at 2216.547 kb on - strand, within DvMF_1815 at 2216.714 kb on + strand, within DvMF_1815 at 2216.722 kb on - strand, within DvMF_1815 at 2216.740 kb on + strand, within DvMF_1815 at 2216.749 kb on + strand, within DvMF_1815 at 2216.766 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media with D,L-Cysteine remove 2,214,271 + +0.6 2,214,278 + -0.7 2,214,279 - -0.2 2,214,294 - -0.3 2,214,311 - -1.2 2,214,420 + +0.1 2,214,793 + -0.7 2,214,920 - -0.6 2,214,930 - -0.2 2,215,092 - +0.4 2,215,214 - -0.2 2,215,236 - -0.1 2,215,283 - DvMF_1813 0.17 -0.7 2,215,315 + DvMF_1813 0.22 -0.5 2,215,371 + DvMF_1813 0.31 -0.4 2,215,628 - DvMF_1813 0.71 -1.3 2,215,630 + DvMF_1813 0.72 -0.5 2,215,832 - -0.8 2,215,902 - -0.4 2,216,090 - -0.8 2,216,156 - -0.0 2,216,184 - -0.5 2,216,309 - -1.1 2,216,507 + -0.7 2,216,547 - DvMF_1815 0.14 -0.5 2,216,714 + DvMF_1815 0.74 -1.0 2,216,722 - DvMF_1815 0.77 -1.1 2,216,740 + DvMF_1815 0.83 -0.9 2,216,749 + DvMF_1815 0.86 -0.4 2,216,766 + -0.7
Or see this region's nucleotide sequence