Experiment: MoLS4 minimal media with D,L-Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0352 and DvMF_0353 are separated by 285 nucleotides DvMF_0353 and DvMF_0354 are separated by 96 nucleotides DvMF_0354 and DvMF_0355 are separated by 16 nucleotides
DvMF_0352: DvMF_0352 - putative phage repressor (RefSeq), at 409,755 to 410,540
_0352
DvMF_0353: DvMF_0353 - diaminopimelate decarboxylase (RefSeq), at 410,826 to 412,076
_0353
DvMF_0354: DvMF_0354 - hypothetical protein (RefSeq), at 412,173 to 412,694
_0354
DvMF_0355: DvMF_0355 - DNA mismatch repair protein MutS (RefSeq), at 412,711 to 415,443
_0355
Position (kb) 410
411
412
413 Strain fitness (log2 ratio) -1
0
1 at 410.550 kb on - strand at 410.876 kb on - strand at 411.149 kb on - strand, within DvMF_0353 at 411.543 kb on + strand, within DvMF_0353 at 411.604 kb on + strand, within DvMF_0353 at 411.683 kb on + strand, within DvMF_0353 at 411.894 kb on + strand, within DvMF_0353 at 411.995 kb on - strand at 412.093 kb on - strand at 412.120 kb on + strand at 412.253 kb on + strand, within DvMF_0354 at 412.260 kb on + strand, within DvMF_0354 at 412.260 kb on + strand, within DvMF_0354 at 412.789 kb on - strand at 412.826 kb on + strand at 412.834 kb on - strand at 412.959 kb on - strand at 412.987 kb on - strand, within DvMF_0355
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media with D,L-Cysteine remove 410,550 - -0.8 410,876 - -0.1 411,149 - DvMF_0353 0.26 -1.2 411,543 + DvMF_0353 0.57 -0.4 411,604 + DvMF_0353 0.62 -0.4 411,683 + DvMF_0353 0.69 +0.7 411,894 + DvMF_0353 0.85 -0.2 411,995 - -0.3 412,093 - -0.9 412,120 + -0.1 412,253 + DvMF_0354 0.15 -0.6 412,260 + DvMF_0354 0.17 -0.2 412,260 + DvMF_0354 0.17 -0.9 412,789 - -0.6 412,826 + -0.5 412,834 - -0.4 412,959 - +0.0 412,987 - DvMF_0355 0.10 -0.7
Or see this region's nucleotide sequence