Experiment: MoLS4 minimal media with D,L-Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0260 and DvMF_0261 are separated by 279 nucleotides DvMF_0261 and DvMF_0262 are separated by 501 nucleotides DvMF_0262 and DvMF_0263 are separated by 33 nucleotides
DvMF_0260: DvMF_0260 - Thioredoxin-disulfide reductase (RefSeq), at 297,784 to 298,740
_0260
DvMF_0261: DvMF_0261 - putative chemotaxis phosphatase, CheZ (RefSeq), at 299,020 to 299,766
_0261
DvMF_0262: DvMF_0262 - Homocysteine formation from aspartate semialdehyde (NIL/ferredoxin component) (from data) , at 300,268 to 300,711
_0262
DvMF_0263: DvMF_0263 - tol-pal system protein YbgF (RefSeq), at 300,745 to 301,746
_0263
Position (kb)
299
300 Strain fitness (log2 ratio)
-2
-1
0
1 at 298.159 kb on + strand, within DvMF_0260 at 298.159 kb on + strand, within DvMF_0260 at 298.178 kb on - strand, within DvMF_0260 at 298.242 kb on + strand, within DvMF_0260 at 298.768 kb on + strand at 299.150 kb on - strand, within DvMF_0261 at 299.212 kb on + strand, within DvMF_0261 at 299.212 kb on + strand, within DvMF_0261 at 299.311 kb on + strand, within DvMF_0261 at 299.406 kb on + strand, within DvMF_0261 at 299.518 kb on - strand, within DvMF_0261 at 299.770 kb on - strand at 299.938 kb on + strand at 299.938 kb on + strand at 299.983 kb on - strand at 300.141 kb on - strand at 300.256 kb on - strand at 300.311 kb on + strand at 300.328 kb on - strand, within DvMF_0262 at 300.521 kb on - strand, within DvMF_0262 at 300.606 kb on + strand, within DvMF_0262 at 300.689 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media with D,L-Cysteine remove 298,159 + DvMF_0260 0.39 +0.2 298,159 + DvMF_0260 0.39 +0.3 298,178 - DvMF_0260 0.41 +0.0 298,242 + DvMF_0260 0.48 -1.7 298,768 + +0.1 299,150 - DvMF_0261 0.17 -0.6 299,212 + DvMF_0261 0.26 -1.0 299,212 + DvMF_0261 0.26 -0.8 299,311 + DvMF_0261 0.39 -0.7 299,406 + DvMF_0261 0.52 -0.7 299,518 - DvMF_0261 0.67 -1.0 299,770 - -0.0 299,938 + -0.7 299,938 + -0.6 299,983 - -0.2 300,141 - -0.1 300,256 - -0.4 300,311 + -1.9 300,328 - DvMF_0262 0.14 -1.1 300,521 - DvMF_0262 0.57 -0.3 300,606 + DvMF_0262 0.76 -0.9 300,689 + -1.4
Or see this region's nucleotide sequence