Experiment: BHIS with Dimetridazole 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0672 and BT0673 are separated by 90 nucleotides BT0673 and BT0674 are separated by 176 nucleotides
BT0672: BT0672 - drug efflux protein (NCBI ptt file), at 830,248 to 833,493
BT0672
BT0673: BT0673 - NADP(H) oxidoreductase (NCBI ptt file), at 833,584 to 834,138
BT0673
BT0674: BT0674 - carboxynorspermidine decarboxylase (NCBI ptt file), at 834,315 to 835,454
BT0674
Position (kb) 833
834
835 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 832.620 kb on + strand, within BT0672 at 832.633 kb on + strand, within BT0672 at 832.724 kb on + strand, within BT0672 at 832.724 kb on + strand, within BT0672 at 832.858 kb on + strand, within BT0672 at 832.865 kb on - strand, within BT0672 at 832.866 kb on + strand, within BT0672 at 832.943 kb on - strand, within BT0672 at 832.945 kb on - strand, within BT0672 at 833.025 kb on + strand, within BT0672 at 833.040 kb on + strand, within BT0672 at 833.040 kb on + strand, within BT0672 at 833.292 kb on + strand at 833.397 kb on + strand at 833.398 kb on - strand at 833.415 kb on - strand at 833.509 kb on - strand at 833.516 kb on + strand at 833.517 kb on - strand at 833.553 kb on + strand at 833.557 kb on + strand at 833.571 kb on + strand at 833.573 kb on + strand at 833.580 kb on + strand at 833.581 kb on - strand at 833.640 kb on - strand, within BT0673 at 833.798 kb on + strand, within BT0673 at 833.799 kb on - strand, within BT0673 at 833.828 kb on - strand, within BT0673 at 833.943 kb on - strand, within BT0673 at 834.154 kb on + strand at 834.348 kb on + strand at 834.353 kb on + strand at 834.354 kb on - strand at 834.354 kb on - strand at 834.511 kb on - strand, within BT0674 at 834.623 kb on + strand, within BT0674 at 834.737 kb on + strand, within BT0674 at 834.888 kb on + strand, within BT0674 at 835.047 kb on - strand, within BT0674
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.0125 mM remove 832,620 + BT0672 0.73 +0.7 832,633 + BT0672 0.73 +0.7 832,724 + BT0672 0.76 -0.3 832,724 + BT0672 0.76 -0.7 832,858 + BT0672 0.80 -0.1 832,865 - BT0672 0.81 -2.8 832,866 + BT0672 0.81 -0.9 832,943 - BT0672 0.83 -0.1 832,945 - BT0672 0.83 -1.1 833,025 + BT0672 0.86 -0.6 833,040 + BT0672 0.86 -0.6 833,040 + BT0672 0.86 -1.7 833,292 + -1.7 833,397 + -0.9 833,398 - -1.7 833,415 - -1.5 833,509 - +0.5 833,516 + -1.2 833,517 - -3.2 833,553 + -0.3 833,557 + -0.3 833,571 + -0.2 833,573 + -0.3 833,580 + +2.7 833,581 - -1.0 833,640 - BT0673 0.10 +0.4 833,798 + BT0673 0.39 +0.5 833,799 - BT0673 0.39 -1.3 833,828 - BT0673 0.44 +0.7 833,943 - BT0673 0.65 -0.3 834,154 + -0.3 834,348 + -1.3 834,353 + +0.7 834,354 - -0.3 834,354 - -1.2 834,511 - BT0674 0.17 +0.1 834,623 + BT0674 0.27 -1.9 834,737 + BT0674 0.37 -2.1 834,888 + BT0674 0.50 -0.9 835,047 - BT0674 0.64 -1.9
Or see this region's nucleotide sequence