Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_94 and Pf6N2E2_95 are separated by 109 nucleotides Pf6N2E2_95 and Pf6N2E2_96 are separated by 4 nucleotides Pf6N2E2_96 and Pf6N2E2_97 are separated by 93 nucleotides
Pf6N2E2_94 - Cyn operon transcriptional activator, at 139,673 to 140,587
_94
Pf6N2E2_95 - putative oxygenase (putative secreted protein), at 140,697 to 141,782
_95
Pf6N2E2_96 - FIG011065: hypothetical protein, at 141,787 to 142,551
_96
Pf6N2E2_97 - putataive branched-chain amino acid transport protein AzlC, at 142,645 to 143,355
_97
Position (kb)
140
141
142 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 139.797 kb on - strand, within Pf6N2E2_94 at 139.797 kb on - strand, within Pf6N2E2_94 at 140.071 kb on - strand, within Pf6N2E2_94 at 140.085 kb on + strand, within Pf6N2E2_94 at 140.086 kb on - strand, within Pf6N2E2_94 at 140.086 kb on - strand, within Pf6N2E2_94 at 140.100 kb on + strand, within Pf6N2E2_94 at 140.100 kb on + strand, within Pf6N2E2_94 at 140.101 kb on - strand, within Pf6N2E2_94 at 140.575 kb on + strand at 140.575 kb on + strand at 140.575 kb on + strand at 140.614 kb on + strand at 140.615 kb on - strand at 140.615 kb on - strand at 140.615 kb on - strand at 140.675 kb on + strand at 140.675 kb on + strand at 140.676 kb on - strand at 140.676 kb on - strand at 140.676 kb on - strand at 140.676 kb on - strand at 140.676 kb on - strand at 140.679 kb on + strand at 140.680 kb on - strand at 140.680 kb on - strand at 140.696 kb on + strand at 140.696 kb on + strand at 140.696 kb on + strand at 140.696 kb on + strand at 140.697 kb on - strand at 140.697 kb on - strand at 140.697 kb on - strand at 140.697 kb on - strand at 140.809 kb on + strand, within Pf6N2E2_95 at 140.810 kb on - strand at 141.033 kb on + strand, within Pf6N2E2_95 at 141.148 kb on + strand, within Pf6N2E2_95 at 141.148 kb on + strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.149 kb on - strand, within Pf6N2E2_95 at 141.292 kb on - strand, within Pf6N2E2_95 at 141.507 kb on + strand, within Pf6N2E2_95 at 141.507 kb on + strand, within Pf6N2E2_95 at 141.593 kb on - strand, within Pf6N2E2_95 at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.806 kb on + strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 141.807 kb on - strand at 142.107 kb on - strand, within Pf6N2E2_96 at 142.107 kb on - strand, within Pf6N2E2_96 at 142.126 kb on + strand, within Pf6N2E2_96 at 142.127 kb on - strand, within Pf6N2E2_96 at 142.229 kb on - strand, within Pf6N2E2_96 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.390 kb on + strand, within Pf6N2E2_96 at 142.391 kb on - strand, within Pf6N2E2_96 at 142.528 kb on + strand at 142.528 kb on + strand at 142.528 kb on + strand at 142.528 kb on + strand at 142.529 kb on - strand at 142.560 kb on + strand at 142.602 kb on - strand at 142.602 kb on - strand at 142.602 kb on - strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.621 kb on + strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.622 kb on - strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.693 kb on + strand at 142.694 kb on - strand at 142.694 kb on - strand at 142.694 kb on - strand at 142.694 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 139,797 - Pf6N2E2_94 0.14 -1.5 139,797 - Pf6N2E2_94 0.14 -0.0 140,071 - Pf6N2E2_94 0.43 +1.1 140,085 + Pf6N2E2_94 0.45 +0.9 140,086 - Pf6N2E2_94 0.45 -0.2 140,086 - Pf6N2E2_94 0.45 +0.6 140,100 + Pf6N2E2_94 0.47 -0.5 140,100 + Pf6N2E2_94 0.47 -0.2 140,101 - Pf6N2E2_94 0.47 +0.2 140,575 + -1.2 140,575 + -0.0 140,575 + -1.5 140,614 + +0.7 140,615 - -1.5 140,615 - -0.2 140,615 - -1.4 140,675 + -0.9 140,675 + -0.2 140,676 - +0.4 140,676 - +0.8 140,676 - -0.4 140,676 - +0.3 140,676 - -0.1 140,679 + +1.7 140,680 - +0.3 140,680 - +1.1 140,696 + -0.9 140,696 + +1.9 140,696 + +0.1 140,696 + +1.8 140,697 - -0.6 140,697 - -0.6 140,697 - +0.1 140,697 - -0.3 140,809 + Pf6N2E2_95 0.10 +1.5 140,810 - +1.7 141,033 + Pf6N2E2_95 0.31 +0.3 141,148 + Pf6N2E2_95 0.42 +0.0 141,148 + Pf6N2E2_95 0.42 -1.9 141,149 - Pf6N2E2_95 0.42 +0.5 141,149 - Pf6N2E2_95 0.42 -0.3 141,149 - Pf6N2E2_95 0.42 -1.3 141,149 - Pf6N2E2_95 0.42 -0.3 141,149 - Pf6N2E2_95 0.42 -0.6 141,149 - Pf6N2E2_95 0.42 -0.3 141,292 - Pf6N2E2_95 0.55 -0.6 141,507 + Pf6N2E2_95 0.75 -3.3 141,507 + Pf6N2E2_95 0.75 -1.5 141,593 - Pf6N2E2_95 0.83 -0.6 141,806 + -0.1 141,806 + +0.2 141,806 + -0.1 141,806 + -0.7 141,806 + -0.9 141,806 + -0.9 141,806 + -0.7 141,807 - +1.0 141,807 - -1.3 141,807 - +0.1 141,807 - +0.7 141,807 - +0.6 141,807 - +1.0 141,807 - -0.6 141,807 - +0.3 141,807 - -1.6 142,107 - Pf6N2E2_96 0.42 -0.7 142,107 - Pf6N2E2_96 0.42 -1.9 142,126 + Pf6N2E2_96 0.44 -0.8 142,127 - Pf6N2E2_96 0.44 -0.0 142,229 - Pf6N2E2_96 0.58 +2.7 142,390 + Pf6N2E2_96 0.79 +0.5 142,390 + Pf6N2E2_96 0.79 -2.5 142,390 + Pf6N2E2_96 0.79 -2.5 142,391 - Pf6N2E2_96 0.79 +1.3 142,528 + +0.9 142,528 + +1.4 142,528 + -0.6 142,528 + +0.5 142,529 - +1.5 142,560 + -2.6 142,602 - +1.1 142,602 - +0.4 142,602 - -0.1 142,621 + +0.7 142,621 + +0.8 142,621 + -0.5 142,621 + +2.7 142,622 - +1.0 142,622 - -0.3 142,622 - +0.0 142,622 - -0.4 142,622 - -1.0 142,622 - -0.5 142,622 - -1.9 142,693 + -0.3 142,693 + +0.4 142,693 + -1.4 142,693 + +0.9 142,693 + -0.9 142,693 + +0.7 142,694 - -0.0 142,694 - -0.0 142,694 - -0.1 142,694 - +0.4
Or see this region's nucleotide sequence