Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6 and Pf6N2E2_7 are separated by 240 nucleotides Pf6N2E2_7 and Pf6N2E2_8 are separated by 265 nucleotides Pf6N2E2_8 and Pf6N2E2_9 are separated by 204 nucleotides
Pf6N2E2_6 - Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39), at 27,994 to 31,221
_6
Pf6N2E2_7 - hypothetical protein, at 31,462 to 31,596
_7
Pf6N2E2_8 - TonB-dependent siderophore receptor, at 31,862 to 34,336
_8
Pf6N2E2_9 - Pyoverdine sidechain non-ribosomal peptide synthetase PvdD @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 34,541 to 38,686
_9
Position (kb) 31
32
33
34
35 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 31.190 kb on + strand at 31.190 kb on + strand at 31.190 kb on + strand at 31.190 kb on + strand at 31.191 kb on - strand at 31.277 kb on + strand at 31.277 kb on + strand at 31.312 kb on + strand at 31.312 kb on + strand at 31.312 kb on + strand at 31.312 kb on + strand at 31.312 kb on + strand at 31.312 kb on + strand at 31.313 kb on - strand at 31.313 kb on - strand at 31.313 kb on - strand at 31.363 kb on + strand at 31.364 kb on - strand at 31.409 kb on - strand at 31.636 kb on + strand at 31.640 kb on + strand at 31.640 kb on + strand at 31.641 kb on - strand at 31.641 kb on - strand at 31.641 kb on - strand at 31.644 kb on + strand at 31.644 kb on + strand at 31.644 kb on + strand at 31.645 kb on - strand at 31.645 kb on - strand at 31.645 kb on - strand at 31.645 kb on - strand at 31.645 kb on - strand at 31.645 kb on - strand at 31.842 kb on + strand at 31.843 kb on - strand at 31.843 kb on - strand at 31.848 kb on + strand at 31.849 kb on - strand at 31.851 kb on + strand at 31.852 kb on - strand at 31.852 kb on - strand at 32.075 kb on + strand at 32.075 kb on + strand at 32.075 kb on + strand at 32.076 kb on - strand at 32.076 kb on - strand at 32.194 kb on + strand, within Pf6N2E2_8 at 32.298 kb on - strand, within Pf6N2E2_8 at 32.540 kb on + strand, within Pf6N2E2_8 at 32.541 kb on - strand, within Pf6N2E2_8 at 32.849 kb on + strand, within Pf6N2E2_8 at 33.035 kb on + strand, within Pf6N2E2_8 at 33.036 kb on - strand, within Pf6N2E2_8 at 33.036 kb on - strand, within Pf6N2E2_8 at 33.039 kb on - strand, within Pf6N2E2_8 at 33.039 kb on - strand, within Pf6N2E2_8 at 33.039 kb on - strand, within Pf6N2E2_8 at 33.179 kb on + strand, within Pf6N2E2_8 at 33.179 kb on + strand, within Pf6N2E2_8 at 33.180 kb on - strand, within Pf6N2E2_8 at 33.313 kb on + strand, within Pf6N2E2_8 at 33.390 kb on - strand, within Pf6N2E2_8 at 33.390 kb on - strand, within Pf6N2E2_8 at 33.437 kb on + strand, within Pf6N2E2_8 at 33.461 kb on + strand, within Pf6N2E2_8 at 33.462 kb on - strand, within Pf6N2E2_8 at 33.527 kb on + strand, within Pf6N2E2_8 at 33.528 kb on - strand, within Pf6N2E2_8 at 33.572 kb on - strand, within Pf6N2E2_8 at 33.638 kb on + strand, within Pf6N2E2_8 at 33.638 kb on + strand, within Pf6N2E2_8 at 33.638 kb on + strand, within Pf6N2E2_8 at 33.638 kb on + strand, within Pf6N2E2_8 at 33.638 kb on + strand, within Pf6N2E2_8 at 33.638 kb on + strand, within Pf6N2E2_8 at 33.639 kb on - strand, within Pf6N2E2_8 at 33.639 kb on - strand, within Pf6N2E2_8 at 33.639 kb on - strand, within Pf6N2E2_8 at 33.639 kb on - strand, within Pf6N2E2_8 at 33.639 kb on - strand, within Pf6N2E2_8 at 33.639 kb on - strand, within Pf6N2E2_8 at 33.639 kb on - strand, within Pf6N2E2_8 at 33.674 kb on + strand, within Pf6N2E2_8 at 33.674 kb on + strand, within Pf6N2E2_8 at 33.675 kb on - strand, within Pf6N2E2_8 at 33.675 kb on - strand, within Pf6N2E2_8 at 33.749 kb on + strand, within Pf6N2E2_8 at 33.749 kb on + strand, within Pf6N2E2_8 at 33.749 kb on + strand, within Pf6N2E2_8 at 33.750 kb on - strand, within Pf6N2E2_8 at 33.750 kb on - strand, within Pf6N2E2_8 at 33.750 kb on - strand, within Pf6N2E2_8 at 33.750 kb on - strand, within Pf6N2E2_8 at 33.793 kb on + strand, within Pf6N2E2_8 at 33.794 kb on - strand, within Pf6N2E2_8 at 33.794 kb on - strand, within Pf6N2E2_8 at 33.794 kb on - strand, within Pf6N2E2_8 at 33.794 kb on - strand, within Pf6N2E2_8 at 33.853 kb on + strand, within Pf6N2E2_8 at 33.962 kb on + strand, within Pf6N2E2_8 at 33.962 kb on + strand, within Pf6N2E2_8 at 33.962 kb on + strand, within Pf6N2E2_8 at 33.962 kb on + strand, within Pf6N2E2_8 at 33.962 kb on + strand, within Pf6N2E2_8 at 33.962 kb on + strand, within Pf6N2E2_8 at 33.962 kb on + strand, within Pf6N2E2_8 at 33.963 kb on - strand, within Pf6N2E2_8 at 33.963 kb on - strand, within Pf6N2E2_8 at 33.963 kb on - strand, within Pf6N2E2_8 at 34.016 kb on - strand, within Pf6N2E2_8 at 34.052 kb on - strand, within Pf6N2E2_8 at 34.052 kb on - strand, within Pf6N2E2_8 at 34.055 kb on + strand, within Pf6N2E2_8 at 34.055 kb on + strand, within Pf6N2E2_8 at 34.128 kb on - strand at 34.133 kb on + strand at 34.133 kb on + strand at 34.134 kb on - strand at 34.134 kb on - strand at 34.154 kb on + strand at 34.241 kb on + strand at 34.241 kb on + strand at 34.241 kb on + strand at 34.241 kb on + strand at 34.242 kb on - strand at 34.257 kb on - strand at 34.319 kb on + strand at 34.320 kb on - strand at 34.386 kb on - strand at 34.543 kb on - strand at 34.543 kb on - strand at 34.546 kb on + strand at 34.546 kb on + strand at 34.546 kb on + strand at 34.547 kb on - strand at 34.547 kb on - strand at 34.547 kb on - strand at 34.547 kb on - strand at 34.547 kb on - strand at 34.547 kb on - strand at 34.547 kb on - strand at 34.667 kb on - strand at 34.667 kb on - strand at 34.667 kb on - strand at 34.669 kb on - strand at 34.738 kb on + strand at 34.939 kb on - strand at 34.983 kb on + strand, within Pf6N2E2_9 at 34.983 kb on + strand, within Pf6N2E2_9 at 34.983 kb on + strand, within Pf6N2E2_9 at 34.984 kb on - strand, within Pf6N2E2_9 at 34.984 kb on - strand, within Pf6N2E2_9 at 35.119 kb on + strand, within Pf6N2E2_9 at 35.190 kb on + strand, within Pf6N2E2_9 at 35.191 kb on - strand, within Pf6N2E2_9 at 35.191 kb on - strand, within Pf6N2E2_9 at 35.191 kb on - strand, within Pf6N2E2_9 at 35.193 kb on + strand, within Pf6N2E2_9
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 31,190 + -0.3 31,190 + -1.3 31,190 + -0.1 31,190 + +0.8 31,191 - -0.5 31,277 + -0.9 31,277 + -1.0 31,312 + +0.2 31,312 + +1.5 31,312 + -0.2 31,312 + -0.6 31,312 + -0.4 31,312 + -0.6 31,313 - -0.5 31,313 - -0.8 31,313 - +0.7 31,363 + +0.4 31,364 - +0.3 31,409 - -0.7 31,636 + -2.2 31,640 + -0.3 31,640 + -0.9 31,641 - +0.9 31,641 - +0.1 31,641 - +0.2 31,644 + -0.6 31,644 + +0.4 31,644 + -0.5 31,645 - +1.4 31,645 - +0.2 31,645 - -0.7 31,645 - +0.7 31,645 - +0.4 31,645 - +0.8 31,842 + -0.1 31,843 - -0.7 31,843 - -0.9 31,848 + +0.9 31,849 - -0.6 31,851 + +0.1 31,852 - +0.9 31,852 - +0.4 32,075 + +0.1 32,075 + +0.2 32,075 + +0.7 32,076 - +0.1 32,076 - +1.0 32,194 + Pf6N2E2_8 0.13 -0.5 32,298 - Pf6N2E2_8 0.18 -1.1 32,540 + Pf6N2E2_8 0.27 +0.3 32,541 - Pf6N2E2_8 0.27 +0.1 32,849 + Pf6N2E2_8 0.40 -0.6 33,035 + Pf6N2E2_8 0.47 +0.1 33,036 - Pf6N2E2_8 0.47 +0.5 33,036 - Pf6N2E2_8 0.47 -0.0 33,039 - Pf6N2E2_8 0.48 +0.5 33,039 - Pf6N2E2_8 0.48 -0.5 33,039 - Pf6N2E2_8 0.48 -1.2 33,179 + Pf6N2E2_8 0.53 +0.4 33,179 + Pf6N2E2_8 0.53 +1.9 33,180 - Pf6N2E2_8 0.53 -0.1 33,313 + Pf6N2E2_8 0.59 +1.9 33,390 - Pf6N2E2_8 0.62 +0.8 33,390 - Pf6N2E2_8 0.62 +0.3 33,437 + Pf6N2E2_8 0.64 +0.6 33,461 + Pf6N2E2_8 0.65 -0.5 33,462 - Pf6N2E2_8 0.65 -0.4 33,527 + Pf6N2E2_8 0.67 +1.1 33,528 - Pf6N2E2_8 0.67 +0.6 33,572 - Pf6N2E2_8 0.69 -0.5 33,638 + Pf6N2E2_8 0.72 +0.7 33,638 + Pf6N2E2_8 0.72 -0.2 33,638 + Pf6N2E2_8 0.72 -1.5 33,638 + Pf6N2E2_8 0.72 +0.7 33,638 + Pf6N2E2_8 0.72 +1.2 33,638 + Pf6N2E2_8 0.72 -1.3 33,639 - Pf6N2E2_8 0.72 -0.0 33,639 - Pf6N2E2_8 0.72 -0.5 33,639 - Pf6N2E2_8 0.72 -0.1 33,639 - Pf6N2E2_8 0.72 -0.7 33,639 - Pf6N2E2_8 0.72 +0.2 33,639 - Pf6N2E2_8 0.72 -1.8 33,639 - Pf6N2E2_8 0.72 -0.7 33,674 + Pf6N2E2_8 0.73 -0.2 33,674 + Pf6N2E2_8 0.73 +0.8 33,675 - Pf6N2E2_8 0.73 -0.6 33,675 - Pf6N2E2_8 0.73 +0.0 33,749 + Pf6N2E2_8 0.76 +0.3 33,749 + Pf6N2E2_8 0.76 +0.2 33,749 + Pf6N2E2_8 0.76 -0.4 33,750 - Pf6N2E2_8 0.76 -1.2 33,750 - Pf6N2E2_8 0.76 +0.1 33,750 - Pf6N2E2_8 0.76 +0.3 33,750 - Pf6N2E2_8 0.76 -1.3 33,793 + Pf6N2E2_8 0.78 -0.3 33,794 - Pf6N2E2_8 0.78 -0.0 33,794 - Pf6N2E2_8 0.78 +0.4 33,794 - Pf6N2E2_8 0.78 +0.1 33,794 - Pf6N2E2_8 0.78 -0.2 33,853 + Pf6N2E2_8 0.80 +0.2 33,962 + Pf6N2E2_8 0.85 -1.0 33,962 + Pf6N2E2_8 0.85 -0.3 33,962 + Pf6N2E2_8 0.85 +0.0 33,962 + Pf6N2E2_8 0.85 -0.9 33,962 + Pf6N2E2_8 0.85 -1.9 33,962 + Pf6N2E2_8 0.85 -0.9 33,962 + Pf6N2E2_8 0.85 -0.2 33,963 - Pf6N2E2_8 0.85 -0.4 33,963 - Pf6N2E2_8 0.85 +0.4 33,963 - Pf6N2E2_8 0.85 +0.5 34,016 - Pf6N2E2_8 0.87 -2.5 34,052 - Pf6N2E2_8 0.88 -3.7 34,052 - Pf6N2E2_8 0.88 -0.7 34,055 + Pf6N2E2_8 0.89 -1.2 34,055 + Pf6N2E2_8 0.89 +0.4 34,128 - -0.2 34,133 + -1.5 34,133 + -0.6 34,134 - -0.1 34,134 - +0.6 34,154 + -1.0 34,241 + -1.2 34,241 + +0.2 34,241 + +1.1 34,241 + -0.3 34,242 - -0.5 34,257 - -3.4 34,319 + +0.4 34,320 - -0.1 34,386 - -1.7 34,543 - -0.4 34,543 - -0.9 34,546 + +1.3 34,546 + +0.3 34,546 + +0.1 34,547 - -0.2 34,547 - -0.3 34,547 - +0.2 34,547 - -0.6 34,547 - -0.2 34,547 - +0.5 34,547 - -1.7 34,667 - +0.4 34,667 - -0.1 34,667 - -0.0 34,669 - +0.4 34,738 + -0.9 34,939 - -0.3 34,983 + Pf6N2E2_9 0.11 +0.9 34,983 + Pf6N2E2_9 0.11 -0.5 34,983 + Pf6N2E2_9 0.11 +1.2 34,984 - Pf6N2E2_9 0.11 -0.0 34,984 - Pf6N2E2_9 0.11 +0.4 35,119 + Pf6N2E2_9 0.14 +0.1 35,190 + Pf6N2E2_9 0.16 -0.7 35,191 - Pf6N2E2_9 0.16 +0.8 35,191 - Pf6N2E2_9 0.16 +0.6 35,191 - Pf6N2E2_9 0.16 +1.1 35,193 + Pf6N2E2_9 0.16 -0.3
Or see this region's nucleotide sequence