Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5904 and Pf6N2E2_5905 are separated by 950 nucleotides Pf6N2E2_5905 and Pf6N2E2_5906 are separated by 281 nucleotides Pf6N2E2_5906 and Pf6N2E2_5907 are separated by 158 nucleotides Pf6N2E2_5907 and Pf6N2E2_5908 are separated by 26 nucleotides
Pf6N2E2_5904 - Sorbitol dehydrogenase (EC 1.1.1.14), at 6,723,927 to 6,724,328
_5904
Pf6N2E2_5905 - Transcriptional regulator of various polyols utilization, AraC family, at 6,725,279 to 6,726,148
_5905
Pf6N2E2_5906 - Flp pilus assembly protein, pilin Flp, at 6,726,430 to 6,726,654
_5906
Pf6N2E2_5907 - Flp pilus assembly protein, pilin Flp, at 6,726,813 to 6,727,007
_5907
Pf6N2E2_5908 - Type IV prepilin peptidase TadV/CpaA, at 6,727,034 to 6,727,564
_5908
Position (kb)
6725
6726
6727 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6724.382 kb on + strand at 6724.382 kb on + strand at 6724.382 kb on + strand at 6724.382 kb on + strand at 6724.382 kb on + strand at 6724.382 kb on + strand at 6724.382 kb on + strand at 6724.382 kb on + strand at 6724.382 kb on + strand at 6724.383 kb on - strand at 6724.383 kb on - strand at 6724.407 kb on + strand at 6724.408 kb on - strand at 6724.408 kb on - strand at 6724.496 kb on + strand at 6724.558 kb on - strand at 6724.558 kb on - strand at 6724.558 kb on - strand at 6724.595 kb on + strand at 6724.650 kb on + strand at 6724.650 kb on + strand at 6724.650 kb on + strand at 6724.651 kb on - strand at 6724.651 kb on - strand at 6724.708 kb on - strand at 6724.857 kb on + strand at 6724.858 kb on - strand at 6724.858 kb on - strand at 6724.858 kb on - strand at 6724.872 kb on + strand at 6724.872 kb on + strand at 6724.872 kb on + strand at 6724.872 kb on + strand at 6724.873 kb on - strand at 6724.874 kb on + strand at 6724.875 kb on - strand at 6724.956 kb on - strand at 6725.003 kb on + strand at 6725.003 kb on + strand at 6725.003 kb on + strand at 6725.004 kb on - strand at 6725.004 kb on - strand at 6725.004 kb on - strand at 6725.022 kb on + strand at 6725.080 kb on + strand at 6725.080 kb on + strand at 6725.120 kb on + strand at 6725.201 kb on + strand at 6725.221 kb on + strand at 6725.222 kb on - strand at 6725.222 kb on - strand at 6725.228 kb on - strand at 6725.371 kb on - strand, within Pf6N2E2_5905 at 6725.406 kb on + strand, within Pf6N2E2_5905 at 6725.430 kb on + strand, within Pf6N2E2_5905 at 6725.430 kb on + strand, within Pf6N2E2_5905 at 6725.430 kb on + strand, within Pf6N2E2_5905 at 6725.532 kb on + strand, within Pf6N2E2_5905 at 6725.532 kb on + strand, within Pf6N2E2_5905 at 6725.533 kb on - strand, within Pf6N2E2_5905 at 6725.652 kb on + strand, within Pf6N2E2_5905 at 6725.652 kb on + strand, within Pf6N2E2_5905 at 6725.652 kb on + strand, within Pf6N2E2_5905 at 6725.653 kb on - strand, within Pf6N2E2_5905 at 6725.653 kb on - strand, within Pf6N2E2_5905 at 6725.844 kb on + strand, within Pf6N2E2_5905 at 6725.844 kb on + strand, within Pf6N2E2_5905 at 6725.844 kb on + strand, within Pf6N2E2_5905 at 6725.844 kb on + strand, within Pf6N2E2_5905 at 6725.844 kb on + strand, within Pf6N2E2_5905 at 6725.844 kb on + strand, within Pf6N2E2_5905 at 6725.845 kb on - strand, within Pf6N2E2_5905 at 6725.845 kb on - strand, within Pf6N2E2_5905 at 6725.845 kb on - strand, within Pf6N2E2_5905 at 6725.927 kb on + strand, within Pf6N2E2_5905 at 6726.148 kb on + strand at 6726.149 kb on - strand at 6726.182 kb on - strand at 6726.182 kb on - strand at 6726.182 kb on - strand at 6726.192 kb on - strand at 6726.206 kb on - strand at 6726.206 kb on - strand at 6726.396 kb on - strand at 6726.422 kb on + strand at 6726.422 kb on + strand at 6726.422 kb on + strand at 6726.422 kb on + strand at 6726.422 kb on + strand at 6726.423 kb on - strand at 6726.489 kb on - strand, within Pf6N2E2_5906 at 6726.560 kb on + strand, within Pf6N2E2_5906 at 6726.560 kb on + strand, within Pf6N2E2_5906 at 6726.687 kb on - strand at 6726.700 kb on + strand at 6726.727 kb on + strand at 6726.778 kb on + strand at 6726.778 kb on + strand at 6726.784 kb on - strand at 6726.829 kb on + strand at 6726.829 kb on + strand at 6726.829 kb on + strand at 6726.829 kb on + strand at 6726.829 kb on + strand at 6726.830 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 6,724,382 + -0.3 6,724,382 + +1.4 6,724,382 + +1.4 6,724,382 + -0.4 6,724,382 + -0.6 6,724,382 + -0.2 6,724,382 + +0.8 6,724,382 + +0.5 6,724,382 + +0.1 6,724,383 - -0.1 6,724,383 - -0.3 6,724,407 + +2.2 6,724,408 - +0.1 6,724,408 - -0.6 6,724,496 + -0.3 6,724,558 - +0.0 6,724,558 - -1.3 6,724,558 - +1.4 6,724,595 + +0.4 6,724,650 + +0.3 6,724,650 + +0.0 6,724,650 + -0.7 6,724,651 - +0.8 6,724,651 - -0.2 6,724,708 - -0.1 6,724,857 + +1.3 6,724,858 - -0.1 6,724,858 - +0.2 6,724,858 - +1.2 6,724,872 + +0.5 6,724,872 + -0.6 6,724,872 + -1.0 6,724,872 + +1.3 6,724,873 - -0.4 6,724,874 + +0.4 6,724,875 - -0.2 6,724,956 - +0.6 6,725,003 + +3.5 6,725,003 + +0.6 6,725,003 + +0.6 6,725,004 - +0.2 6,725,004 - -0.1 6,725,004 - +0.9 6,725,022 + -0.1 6,725,080 + +0.6 6,725,080 + +0.8 6,725,120 + +0.1 6,725,201 + +1.0 6,725,221 + +0.1 6,725,222 - +1.3 6,725,222 - +0.5 6,725,228 - +0.3 6,725,371 - Pf6N2E2_5905 0.11 +1.8 6,725,406 + Pf6N2E2_5905 0.15 +0.5 6,725,430 + Pf6N2E2_5905 0.17 +1.7 6,725,430 + Pf6N2E2_5905 0.17 +1.4 6,725,430 + Pf6N2E2_5905 0.17 -0.4 6,725,532 + Pf6N2E2_5905 0.29 +2.0 6,725,532 + Pf6N2E2_5905 0.29 -0.2 6,725,533 - Pf6N2E2_5905 0.29 +0.4 6,725,652 + Pf6N2E2_5905 0.43 +0.4 6,725,652 + Pf6N2E2_5905 0.43 +0.1 6,725,652 + Pf6N2E2_5905 0.43 +0.5 6,725,653 - Pf6N2E2_5905 0.43 +1.5 6,725,653 - Pf6N2E2_5905 0.43 +1.9 6,725,844 + Pf6N2E2_5905 0.65 -0.7 6,725,844 + Pf6N2E2_5905 0.65 +1.2 6,725,844 + Pf6N2E2_5905 0.65 -2.5 6,725,844 + Pf6N2E2_5905 0.65 +1.0 6,725,844 + Pf6N2E2_5905 0.65 -1.4 6,725,844 + Pf6N2E2_5905 0.65 +1.9 6,725,845 - Pf6N2E2_5905 0.65 +0.3 6,725,845 - Pf6N2E2_5905 0.65 +0.4 6,725,845 - Pf6N2E2_5905 0.65 +0.5 6,725,927 + Pf6N2E2_5905 0.74 -0.1 6,726,148 + -0.3 6,726,149 - +0.2 6,726,182 - -2.3 6,726,182 - -0.6 6,726,182 - +0.9 6,726,192 - +0.5 6,726,206 - -0.7 6,726,206 - -0.3 6,726,396 - +0.7 6,726,422 + -0.8 6,726,422 + +1.3 6,726,422 + +1.5 6,726,422 + -1.1 6,726,422 + +0.7 6,726,423 - +0.1 6,726,489 - Pf6N2E2_5906 0.26 +1.0 6,726,560 + Pf6N2E2_5906 0.58 +0.3 6,726,560 + Pf6N2E2_5906 0.58 -0.4 6,726,687 - +0.2 6,726,700 + -0.6 6,726,727 + -0.3 6,726,778 + +1.1 6,726,778 + -1.6 6,726,784 - -1.1 6,726,829 + -1.4 6,726,829 + -1.6 6,726,829 + -0.8 6,726,829 + +0.1 6,726,829 + -0.1 6,726,830 - -0.4
Or see this region's nucleotide sequence