Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4639 and Pf6N2E2_4640 are separated by 176 nucleotides Pf6N2E2_4640 and Pf6N2E2_4641 are separated by 23 nucleotides Pf6N2E2_4641 and Pf6N2E2_4642 are separated by 87 nucleotides
Pf6N2E2_4639 - Adenosylhomocysteinase (EC 3.3.1.1), at 5,368,863 to 5,370,272
_4639
Pf6N2E2_4640 - hypothetical protein, at 5,370,449 to 5,370,571
_4640
Pf6N2E2_4641 - cytosolic long-chain acyl-CoA thioester hydrolase family protein, at 5,370,595 to 5,370,996
_4641
Pf6N2E2_4642 - Putative transport, at 5,371,084 to 5,371,965
_4642
Position (kb) 5370
5371 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5370.290 kb on - strand at 5370.290 kb on - strand at 5370.290 kb on - strand at 5370.295 kb on - strand at 5370.295 kb on - strand at 5370.295 kb on - strand at 5370.295 kb on - strand at 5370.451 kb on - strand at 5370.453 kb on - strand at 5370.453 kb on - strand at 5370.517 kb on + strand, within Pf6N2E2_4640 at 5370.532 kb on - strand, within Pf6N2E2_4640 at 5370.700 kb on + strand, within Pf6N2E2_4641 at 5370.700 kb on + strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.743 kb on - strand, within Pf6N2E2_4641 at 5370.743 kb on - strand, within Pf6N2E2_4641 at 5370.743 kb on - strand, within Pf6N2E2_4641 at 5370.743 kb on - strand, within Pf6N2E2_4641 at 5370.743 kb on - strand, within Pf6N2E2_4641 at 5370.825 kb on + strand, within Pf6N2E2_4641 at 5370.958 kb on + strand at 5370.958 kb on + strand at 5370.959 kb on - strand at 5370.959 kb on - strand at 5370.959 kb on - strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.053 kb on - strand at 5371.053 kb on - strand at 5371.053 kb on - strand at 5371.056 kb on + strand at 5371.056 kb on + strand at 5371.056 kb on + strand at 5371.056 kb on + strand at 5371.056 kb on + strand at 5371.057 kb on - strand at 5371.057 kb on - strand at 5371.057 kb on - strand at 5371.083 kb on + strand at 5371.083 kb on + strand at 5371.084 kb on - strand at 5371.200 kb on + strand, within Pf6N2E2_4642 at 5371.201 kb on - strand, within Pf6N2E2_4642 at 5371.522 kb on + strand, within Pf6N2E2_4642 at 5371.522 kb on + strand, within Pf6N2E2_4642 at 5371.522 kb on + strand, within Pf6N2E2_4642 at 5371.522 kb on + strand, within Pf6N2E2_4642 at 5371.523 kb on - strand, within Pf6N2E2_4642 at 5371.523 kb on - strand, within Pf6N2E2_4642 at 5371.523 kb on - strand, within Pf6N2E2_4642 at 5371.523 kb on - strand, within Pf6N2E2_4642
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 5,370,290 - -2.9 5,370,290 - +0.3 5,370,290 - -0.1 5,370,295 - -0.0 5,370,295 - -0.7 5,370,295 - -2.0 5,370,295 - -0.7 5,370,451 - -1.1 5,370,453 - -0.5 5,370,453 - +0.6 5,370,517 + Pf6N2E2_4640 0.55 +1.3 5,370,532 - Pf6N2E2_4640 0.67 +0.8 5,370,700 + Pf6N2E2_4641 0.26 +0.3 5,370,700 + Pf6N2E2_4641 0.26 +0.9 5,370,701 - Pf6N2E2_4641 0.26 +1.1 5,370,701 - Pf6N2E2_4641 0.26 -0.9 5,370,701 - Pf6N2E2_4641 0.26 +1.1 5,370,701 - Pf6N2E2_4641 0.26 +0.9 5,370,701 - Pf6N2E2_4641 0.26 +0.4 5,370,701 - Pf6N2E2_4641 0.26 +0.5 5,370,701 - Pf6N2E2_4641 0.26 -0.2 5,370,742 + Pf6N2E2_4641 0.37 +1.3 5,370,742 + Pf6N2E2_4641 0.37 +0.6 5,370,742 + Pf6N2E2_4641 0.37 +1.8 5,370,742 + Pf6N2E2_4641 0.37 +0.4 5,370,742 + Pf6N2E2_4641 0.37 +0.3 5,370,742 + Pf6N2E2_4641 0.37 +0.1 5,370,742 + Pf6N2E2_4641 0.37 +1.2 5,370,743 - Pf6N2E2_4641 0.37 +0.9 5,370,743 - Pf6N2E2_4641 0.37 -2.5 5,370,743 - Pf6N2E2_4641 0.37 +0.5 5,370,743 - Pf6N2E2_4641 0.37 +0.3 5,370,743 - Pf6N2E2_4641 0.37 +1.2 5,370,825 + Pf6N2E2_4641 0.57 +0.3 5,370,958 + +1.2 5,370,958 + +0.1 5,370,959 - +1.5 5,370,959 - +1.4 5,370,959 - +1.1 5,371,052 + +1.4 5,371,052 + +1.2 5,371,052 + +0.9 5,371,052 + -1.3 5,371,052 + -1.0 5,371,052 + +0.8 5,371,052 + +0.5 5,371,053 - +1.2 5,371,053 - +0.6 5,371,053 - -0.3 5,371,056 + +0.6 5,371,056 + +0.5 5,371,056 + -0.8 5,371,056 + -1.3 5,371,056 + -0.5 5,371,057 - +0.7 5,371,057 - +1.2 5,371,057 - -0.8 5,371,083 + -0.6 5,371,083 + -0.2 5,371,084 - +1.9 5,371,200 + Pf6N2E2_4642 0.13 +0.4 5,371,201 - Pf6N2E2_4642 0.13 +0.9 5,371,522 + Pf6N2E2_4642 0.50 -0.5 5,371,522 + Pf6N2E2_4642 0.50 +0.4 5,371,522 + Pf6N2E2_4642 0.50 +0.8 5,371,522 + Pf6N2E2_4642 0.50 +0.8 5,371,523 - Pf6N2E2_4642 0.50 -0.1 5,371,523 - Pf6N2E2_4642 0.50 -0.3 5,371,523 - Pf6N2E2_4642 0.50 +0.1 5,371,523 - Pf6N2E2_4642 0.50 -0.9
Or see this region's nucleotide sequence