Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3816 and Pf6N2E2_3817 are separated by 253 nucleotides Pf6N2E2_3817 and Pf6N2E2_3818 are separated by 151 nucleotides
Pf6N2E2_3816 - Glycogen phosphorylase (EC 2.4.1.1), at 4,449,241 to 4,451,691
_3816
Pf6N2E2_3817 - FIG00955680: hypothetical protein, at 4,451,945 to 4,452,382
_3817
Pf6N2E2_3818 - GTP-binding protein TypA/BipA, at 4,452,534 to 4,454,354
_3818
Position (kb)
4451
4452
4453 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4451.037 kb on - strand, within Pf6N2E2_3816 at 4451.037 kb on - strand, within Pf6N2E2_3816 at 4451.135 kb on + strand, within Pf6N2E2_3816 at 4451.441 kb on + strand, within Pf6N2E2_3816 at 4451.441 kb on + strand, within Pf6N2E2_3816 at 4451.477 kb on + strand at 4451.477 kb on + strand at 4451.477 kb on + strand at 4451.477 kb on + strand at 4451.477 kb on + strand at 4451.478 kb on - strand at 4451.478 kb on - strand at 4451.478 kb on - strand at 4451.478 kb on - strand at 4451.478 kb on - strand at 4451.478 kb on - strand at 4451.478 kb on - strand at 4451.480 kb on + strand at 4451.480 kb on + strand at 4451.480 kb on + strand at 4451.481 kb on - strand at 4451.504 kb on + strand at 4451.505 kb on - strand at 4451.618 kb on + strand at 4451.618 kb on + strand at 4451.618 kb on + strand at 4451.619 kb on - strand at 4451.695 kb on + strand at 4451.696 kb on - strand at 4451.696 kb on - strand at 4451.696 kb on - strand at 4451.749 kb on - strand at 4451.908 kb on + strand at 4451.908 kb on + strand at 4451.936 kb on + strand at 4451.936 kb on + strand at 4451.937 kb on - strand at 4451.993 kb on - strand, within Pf6N2E2_3817 at 4451.993 kb on - strand, within Pf6N2E2_3817 at 4452.005 kb on + strand, within Pf6N2E2_3817 at 4452.005 kb on + strand, within Pf6N2E2_3817 at 4452.006 kb on - strand, within Pf6N2E2_3817 at 4452.006 kb on - strand, within Pf6N2E2_3817 at 4452.006 kb on - strand, within Pf6N2E2_3817 at 4452.170 kb on + strand, within Pf6N2E2_3817 at 4452.171 kb on - strand, within Pf6N2E2_3817 at 4452.227 kb on + strand, within Pf6N2E2_3817 at 4452.228 kb on - strand, within Pf6N2E2_3817 at 4452.228 kb on - strand, within Pf6N2E2_3817 at 4452.260 kb on + strand, within Pf6N2E2_3817 at 4452.260 kb on + strand, within Pf6N2E2_3817 at 4452.260 kb on + strand, within Pf6N2E2_3817 at 4452.261 kb on - strand, within Pf6N2E2_3817 at 4452.317 kb on + strand, within Pf6N2E2_3817 at 4452.318 kb on - strand, within Pf6N2E2_3817 at 4452.326 kb on + strand, within Pf6N2E2_3817 at 4452.326 kb on + strand, within Pf6N2E2_3817 at 4452.327 kb on - strand, within Pf6N2E2_3817 at 4452.327 kb on - strand, within Pf6N2E2_3817 at 4452.373 kb on + strand at 4452.374 kb on - strand at 4452.386 kb on - strand at 4452.391 kb on + strand at 4452.391 kb on + strand at 4452.391 kb on + strand at 4452.392 kb on - strand at 4452.392 kb on - strand at 4452.459 kb on + strand at 4452.459 kb on + strand at 4452.460 kb on - strand at 4452.460 kb on - strand at 4452.491 kb on + strand at 4452.492 kb on - strand at 4452.492 kb on - strand at 4452.512 kb on + strand at 4452.519 kb on + strand at 4452.519 kb on + strand at 4452.519 kb on + strand at 4452.519 kb on + strand at 4452.520 kb on - strand at 4452.520 kb on - strand at 4452.520 kb on - strand at 4452.520 kb on - strand at 4452.520 kb on - strand at 4452.523 kb on + strand at 4452.523 kb on + strand at 4452.523 kb on + strand at 4452.523 kb on + strand at 4452.523 kb on + strand at 4452.524 kb on - strand at 4452.524 kb on - strand at 4452.524 kb on - strand at 4452.524 kb on - strand at 4452.524 kb on - strand at 4452.524 kb on - strand at 4452.524 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,451,037 - Pf6N2E2_3816 0.73 -0.7 4,451,037 - Pf6N2E2_3816 0.73 +0.3 4,451,135 + Pf6N2E2_3816 0.77 +0.2 4,451,441 + Pf6N2E2_3816 0.90 -1.5 4,451,441 + Pf6N2E2_3816 0.90 +0.5 4,451,477 + -0.6 4,451,477 + -2.4 4,451,477 + +0.5 4,451,477 + +0.8 4,451,477 + +0.5 4,451,478 - -2.4 4,451,478 - -0.5 4,451,478 - -0.4 4,451,478 - -1.0 4,451,478 - +0.1 4,451,478 - -0.4 4,451,478 - +2.2 4,451,480 + -0.6 4,451,480 + +0.3 4,451,480 + +0.8 4,451,481 - -0.3 4,451,504 + +1.4 4,451,505 - -0.7 4,451,618 + +1.2 4,451,618 + +0.6 4,451,618 + +0.8 4,451,619 - +1.2 4,451,695 + -0.0 4,451,696 - +1.1 4,451,696 - +1.6 4,451,696 - -1.6 4,451,749 - -0.1 4,451,908 + -0.5 4,451,908 + -0.6 4,451,936 + +0.6 4,451,936 + +0.9 4,451,937 - +0.6 4,451,993 - Pf6N2E2_3817 0.11 +0.3 4,451,993 - Pf6N2E2_3817 0.11 -0.0 4,452,005 + Pf6N2E2_3817 0.14 -0.7 4,452,005 + Pf6N2E2_3817 0.14 +0.0 4,452,006 - Pf6N2E2_3817 0.14 +1.0 4,452,006 - Pf6N2E2_3817 0.14 -1.3 4,452,006 - Pf6N2E2_3817 0.14 +0.1 4,452,170 + Pf6N2E2_3817 0.51 +0.4 4,452,171 - Pf6N2E2_3817 0.52 +0.3 4,452,227 + Pf6N2E2_3817 0.64 +0.3 4,452,228 - Pf6N2E2_3817 0.65 -0.6 4,452,228 - Pf6N2E2_3817 0.65 +1.1 4,452,260 + Pf6N2E2_3817 0.72 +0.3 4,452,260 + Pf6N2E2_3817 0.72 +0.3 4,452,260 + Pf6N2E2_3817 0.72 +0.0 4,452,261 - Pf6N2E2_3817 0.72 +0.8 4,452,317 + Pf6N2E2_3817 0.85 +0.3 4,452,318 - Pf6N2E2_3817 0.85 -0.1 4,452,326 + Pf6N2E2_3817 0.87 -0.1 4,452,326 + Pf6N2E2_3817 0.87 -0.2 4,452,327 - Pf6N2E2_3817 0.87 +0.5 4,452,327 - Pf6N2E2_3817 0.87 +0.6 4,452,373 + +0.4 4,452,374 - -1.8 4,452,386 - +1.5 4,452,391 + -0.2 4,452,391 + -0.9 4,452,391 + +0.4 4,452,392 - -1.1 4,452,392 - +0.8 4,452,459 + +0.4 4,452,459 + +0.0 4,452,460 - +0.4 4,452,460 - +1.0 4,452,491 + -0.1 4,452,492 - +0.0 4,452,492 - +0.6 4,452,512 + -1.2 4,452,519 + -2.1 4,452,519 + +0.5 4,452,519 + +0.3 4,452,519 + +1.2 4,452,520 - +0.2 4,452,520 - +0.3 4,452,520 - -1.1 4,452,520 - +0.3 4,452,520 - +0.1 4,452,523 + -0.5 4,452,523 + +0.6 4,452,523 + +0.9 4,452,523 + -0.6 4,452,523 + -0.1 4,452,524 - -0.3 4,452,524 - +0.5 4,452,524 - -0.2 4,452,524 - -0.1 4,452,524 - -1.9 4,452,524 - -0.0 4,452,524 - -0.4
Or see this region's nucleotide sequence