Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3672 and Pf6N2E2_3673 are separated by 268 nucleotides Pf6N2E2_3673 and Pf6N2E2_3674 overlap by 6 nucleotides Pf6N2E2_3674 and Pf6N2E2_3675 are separated by 11 nucleotides
Pf6N2E2_3672 - Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42), at 4,274,540 to 4,277,491
_3672
Pf6N2E2_3673 - hypothetical protein, at 4,277,760 to 4,277,879
_3673
Pf6N2E2_3674 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 4,277,874 to 4,280,519
_3674
Pf6N2E2_3675 - Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12), at 4,280,531 to 4,282,168
_3675
Position (kb)
4277
4278
4279
4280
4281 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 4277.022 kb on + strand, within Pf6N2E2_3672 at 4277.022 kb on + strand, within Pf6N2E2_3672 at 4277.023 kb on - strand, within Pf6N2E2_3672 at 4277.023 kb on - strand, within Pf6N2E2_3672 at 4277.023 kb on - strand, within Pf6N2E2_3672 at 4277.023 kb on - strand, within Pf6N2E2_3672 at 4277.034 kb on + strand, within Pf6N2E2_3672 at 4277.240 kb on - strand at 4277.279 kb on + strand at 4277.330 kb on - strand at 4277.527 kb on + strand at 4277.543 kb on + strand at 4277.544 kb on - strand at 4277.549 kb on - strand at 4277.549 kb on - strand at 4277.717 kb on + strand at 4277.719 kb on + strand at 4277.719 kb on + strand at 4277.761 kb on + strand at 4278.225 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,277,022 + Pf6N2E2_3672 0.84 -2.2 4,277,022 + Pf6N2E2_3672 0.84 -1.8 4,277,023 - Pf6N2E2_3672 0.84 -0.1 4,277,023 - Pf6N2E2_3672 0.84 +0.7 4,277,023 - Pf6N2E2_3672 0.84 +0.8 4,277,023 - Pf6N2E2_3672 0.84 +1.4 4,277,034 + Pf6N2E2_3672 0.84 -0.9 4,277,240 - +0.2 4,277,279 + +0.1 4,277,330 - +3.9 4,277,527 + -1.5 4,277,543 + -1.0 4,277,544 - +5.9 4,277,549 - +7.3 4,277,549 - +6.9 4,277,717 + -0.8 4,277,719 + -0.3 4,277,719 + -1.4 4,277,761 + -0.7 4,278,225 + +1.4
Or see this region's nucleotide sequence