Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3267

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3265 and Pf6N2E2_3266 overlap by 1 nucleotidesPf6N2E2_3266 and Pf6N2E2_3267 are separated by 188 nucleotidesPf6N2E2_3267 and Pf6N2E2_3268 are separated by 76 nucleotidesPf6N2E2_3268 and Pf6N2E2_3269 are separated by 54 nucleotidesPf6N2E2_3269 and Pf6N2E2_3270 are separated by 2 nucleotides Pf6N2E2_3265 - LptA, protein essential for LPS transport across the periplasm, at 3,771,837 to 3,772,388 _3265 Pf6N2E2_3266 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 3,772,388 to 3,773,113 _3266 Pf6N2E2_3267 - RNA polymerase sigma-54 factor RpoN, at 3,773,302 to 3,774,771 _3267 Pf6N2E2_3268 - Ribosome hibernation protein YhbH, at 3,774,848 to 3,775,156 _3268 Pf6N2E2_3269 - PTS IIA-like nitrogen-regulatory protein PtsN, at 3,775,211 to 3,775,633 _3269 Pf6N2E2_3270 - Hypothetical ATP-binding protein UPF0042, contains P-loop, at 3,775,636 to 3,776,493 _3270 Position (kb) 3773 3774 3775Strain fitness (log2 ratio) -3 -2 -1 0 1at 3773.111 kb on + strandat 3773.111 kb on + strandat 3773.112 kb on - strandat 3773.112 kb on - strandat 3773.149 kb on - strandat 3773.155 kb on - strandat 3773.170 kb on + strandat 3773.170 kb on + strandat 3773.171 kb on - strandat 3773.197 kb on + strandat 3773.230 kb on + strandat 3773.230 kb on + strandat 3773.258 kb on + strandat 3773.258 kb on + strandat 3773.266 kb on + strandat 3774.756 kb on + strandat 3774.769 kb on + strandat 3774.769 kb on + strandat 3774.769 kb on + strandat 3774.847 kb on + strandat 3774.847 kb on + strandat 3774.847 kb on + strandat 3774.847 kb on + strandat 3774.902 kb on + strand, within Pf6N2E2_3268at 3774.902 kb on + strand, within Pf6N2E2_3268at 3774.902 kb on + strand, within Pf6N2E2_3268at 3774.902 kb on + strand, within Pf6N2E2_3268at 3774.902 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.058 kb on + strand, within Pf6N2E2_3268at 3775.631 kb on + strandat 3775.714 kb on + strandat 3775.714 kb on + strandat 3775.714 kb on + strandat 3775.714 kb on + strandat 3775.714 kb on + strandat 3775.715 kb on - strandat 3775.715 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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3,773,111 + -1.0
3,773,111 + +0.5
3,773,112 - -1.1
3,773,112 - -1.8
3,773,149 - -2.0
3,773,155 - -3.3
3,773,170 + -1.1
3,773,170 + -0.8
3,773,171 - -1.8
3,773,197 + +1.3
3,773,230 + -3.6
3,773,230 + -3.4
3,773,258 + -2.6
3,773,258 + -2.8
3,773,266 + -2.3
3,774,756 + -2.8
3,774,769 + -0.6
3,774,769 + +0.0
3,774,769 + -0.5
3,774,847 + -0.8
3,774,847 + +0.4
3,774,847 + +0.5
3,774,847 + +0.4
3,774,902 + Pf6N2E2_3268 0.17 +0.4
3,774,902 + Pf6N2E2_3268 0.17 -0.4
3,774,902 + Pf6N2E2_3268 0.17 +0.5
3,774,902 + Pf6N2E2_3268 0.17 -0.4
3,774,902 + Pf6N2E2_3268 0.17 -2.9
3,775,058 + Pf6N2E2_3268 0.68 -0.2
3,775,058 + Pf6N2E2_3268 0.68 -2.4
3,775,058 + Pf6N2E2_3268 0.68 -0.4
3,775,058 + Pf6N2E2_3268 0.68 -0.2
3,775,058 + Pf6N2E2_3268 0.68 -1.5
3,775,058 + Pf6N2E2_3268 0.68 -0.3
3,775,058 + Pf6N2E2_3268 0.68 -0.4
3,775,058 + Pf6N2E2_3268 0.68 +0.4
3,775,058 + Pf6N2E2_3268 0.68 -1.4
3,775,058 + Pf6N2E2_3268 0.68 -1.3
3,775,058 + Pf6N2E2_3268 0.68 -0.2
3,775,058 + Pf6N2E2_3268 0.68 -2.8
3,775,058 + Pf6N2E2_3268 0.68 -0.4
3,775,058 + Pf6N2E2_3268 0.68 +0.1
3,775,058 + Pf6N2E2_3268 0.68 -0.9
3,775,058 + Pf6N2E2_3268 0.68 -1.4
3,775,058 + Pf6N2E2_3268 0.68 -0.3
3,775,058 + Pf6N2E2_3268 0.68 -1.3
3,775,058 + Pf6N2E2_3268 0.68 -0.3
3,775,631 + -0.1
3,775,714 + -0.0
3,775,714 + -0.3
3,775,714 + -0.2
3,775,714 + -2.0
3,775,714 + -0.8
3,775,715 - -1.8
3,775,715 - -2.9

Or see this region's nucleotide sequence