Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3137 and Pf6N2E2_3138 are separated by 158 nucleotides Pf6N2E2_3138 and Pf6N2E2_3139 are separated by 147 nucleotides Pf6N2E2_3139 and Pf6N2E2_3140 are separated by 234 nucleotides Pf6N2E2_3140 and Pf6N2E2_3141 are separated by 184 nucleotides
Pf6N2E2_3137 - Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-), at 3,628,697 to 3,630,406
_3137
Pf6N2E2_3138 - YaeQ protein, at 3,630,565 to 3,631,107
_3138
Pf6N2E2_3139 - L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16), at 3,631,255 to 3,632,454
_3139
Pf6N2E2_3140 - FIG00953680: hypothetical protein, at 3,632,689 to 3,632,976
_3140
Pf6N2E2_3141 - virulence sensor protein, at 3,633,161 to 3,634,531
_3141
Position (kb)
3631
3632
3633 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3630.330 kb on + strand at 3630.331 kb on - strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.399 kb on - strand at 3630.399 kb on - strand at 3630.399 kb on - strand at 3630.957 kb on - strand, within Pf6N2E2_3138 at 3630.968 kb on + strand, within Pf6N2E2_3138 at 3630.969 kb on - strand, within Pf6N2E2_3138 at 3631.043 kb on + strand, within Pf6N2E2_3138 at 3631.043 kb on + strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.085 kb on + strand at 3631.085 kb on + strand at 3631.085 kb on + strand at 3631.123 kb on + strand at 3631.123 kb on + strand at 3631.123 kb on + strand at 3631.208 kb on - strand at 3631.212 kb on + strand at 3631.212 kb on + strand at 3631.212 kb on + strand at 3631.213 kb on - strand at 3631.213 kb on - strand at 3631.213 kb on - strand at 3631.222 kb on + strand at 3631.415 kb on + strand, within Pf6N2E2_3139 at 3631.415 kb on + strand, within Pf6N2E2_3139 at 3631.415 kb on + strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.418 kb on - strand, within Pf6N2E2_3139 at 3631.418 kb on - strand, within Pf6N2E2_3139 at 3631.418 kb on - strand, within Pf6N2E2_3139 at 3631.712 kb on - strand, within Pf6N2E2_3139 at 3631.864 kb on + strand, within Pf6N2E2_3139 at 3631.865 kb on - strand, within Pf6N2E2_3139 at 3631.865 kb on - strand, within Pf6N2E2_3139 at 3632.125 kb on + strand, within Pf6N2E2_3139 at 3632.126 kb on - strand, within Pf6N2E2_3139 at 3632.229 kb on + strand, within Pf6N2E2_3139 at 3632.229 kb on + strand, within Pf6N2E2_3139 at 3632.230 kb on - strand, within Pf6N2E2_3139 at 3632.522 kb on + strand at 3632.664 kb on + strand at 3632.665 kb on - strand at 3632.665 kb on - strand at 3632.665 kb on - strand at 3632.665 kb on - strand at 3632.776 kb on + strand, within Pf6N2E2_3140 at 3632.777 kb on - strand, within Pf6N2E2_3140 at 3632.777 kb on - strand, within Pf6N2E2_3140 at 3632.777 kb on - strand, within Pf6N2E2_3140 at 3632.934 kb on - strand, within Pf6N2E2_3140 at 3632.959 kb on + strand at 3632.959 kb on + strand at 3632.960 kb on - strand at 3632.967 kb on + strand at 3632.993 kb on - strand at 3633.020 kb on + strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.249 kb on + strand at 3633.249 kb on + strand at 3633.250 kb on - strand at 3633.250 kb on - strand at 3633.250 kb on - strand at 3633.250 kb on - strand at 3633.270 kb on + strand at 3633.271 kb on - strand at 3633.271 kb on - strand at 3633.271 kb on - strand at 3633.438 kb on - strand, within Pf6N2E2_3141 at 3633.451 kb on - strand, within Pf6N2E2_3141
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,630,330 + -0.1 3,630,331 - +0.3 3,630,398 + +1.6 3,630,398 + -0.5 3,630,398 + -1.5 3,630,398 + -1.6 3,630,398 + +0.5 3,630,399 - -0.2 3,630,399 - +0.4 3,630,399 - +0.9 3,630,957 - Pf6N2E2_3138 0.72 +1.7 3,630,968 + Pf6N2E2_3138 0.74 +0.8 3,630,969 - Pf6N2E2_3138 0.74 -0.1 3,631,043 + Pf6N2E2_3138 0.88 +1.3 3,631,043 + Pf6N2E2_3138 0.88 -1.2 3,631,044 - Pf6N2E2_3138 0.88 +1.0 3,631,044 - Pf6N2E2_3138 0.88 +0.8 3,631,044 - Pf6N2E2_3138 0.88 -0.0 3,631,044 - Pf6N2E2_3138 0.88 -1.2 3,631,085 + +0.1 3,631,085 + -1.5 3,631,085 + +0.6 3,631,123 + -0.5 3,631,123 + +0.6 3,631,123 + +0.8 3,631,208 - +1.5 3,631,212 + +0.5 3,631,212 + +1.0 3,631,212 + +0.5 3,631,213 - +0.3 3,631,213 - +1.2 3,631,213 - +0.1 3,631,222 + +1.3 3,631,415 + Pf6N2E2_3139 0.13 -1.2 3,631,415 + Pf6N2E2_3139 0.13 -1.8 3,631,415 + Pf6N2E2_3139 0.13 +0.7 3,631,416 - Pf6N2E2_3139 0.13 -1.5 3,631,416 - Pf6N2E2_3139 0.13 -0.1 3,631,416 - Pf6N2E2_3139 0.13 +0.3 3,631,416 - Pf6N2E2_3139 0.13 -0.9 3,631,416 - Pf6N2E2_3139 0.13 -0.2 3,631,416 - Pf6N2E2_3139 0.13 -0.4 3,631,418 - Pf6N2E2_3139 0.14 +0.2 3,631,418 - Pf6N2E2_3139 0.14 -0.7 3,631,418 - Pf6N2E2_3139 0.14 -1.2 3,631,712 - Pf6N2E2_3139 0.38 +0.6 3,631,864 + Pf6N2E2_3139 0.51 -0.8 3,631,865 - Pf6N2E2_3139 0.51 +1.1 3,631,865 - Pf6N2E2_3139 0.51 -0.6 3,632,125 + Pf6N2E2_3139 0.72 -0.4 3,632,126 - Pf6N2E2_3139 0.73 -1.7 3,632,229 + Pf6N2E2_3139 0.81 +0.3 3,632,229 + Pf6N2E2_3139 0.81 +1.7 3,632,230 - Pf6N2E2_3139 0.81 -0.6 3,632,522 + -0.3 3,632,664 + +0.2 3,632,665 - +0.3 3,632,665 - -0.2 3,632,665 - -0.2 3,632,665 - +0.6 3,632,776 + Pf6N2E2_3140 0.30 +0.9 3,632,777 - Pf6N2E2_3140 0.31 -0.8 3,632,777 - Pf6N2E2_3140 0.31 +0.6 3,632,777 - Pf6N2E2_3140 0.31 -0.4 3,632,934 - Pf6N2E2_3140 0.85 +0.6 3,632,959 + -0.2 3,632,959 + +0.3 3,632,960 - +0.5 3,632,967 + -0.8 3,632,993 - +0.9 3,633,020 + +1.8 3,633,021 - +0.4 3,633,021 - +0.5 3,633,021 - -0.0 3,633,021 - -0.1 3,633,021 - -1.2 3,633,021 - -1.3 3,633,021 - -0.5 3,633,106 + -0.9 3,633,106 + +0.8 3,633,106 + +0.3 3,633,106 + +1.1 3,633,106 + +0.3 3,633,249 + -0.6 3,633,249 + +1.0 3,633,250 - -3.2 3,633,250 - -0.5 3,633,250 - -0.3 3,633,250 - -0.0 3,633,270 + -0.3 3,633,271 - -0.1 3,633,271 - -0.3 3,633,271 - -1.0 3,633,438 - Pf6N2E2_3141 0.20 -0.2 3,633,451 - Pf6N2E2_3141 0.21 +0.1
Or see this region's nucleotide sequence