Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2390 and Pf6N2E2_2391 are separated by 19 nucleotides Pf6N2E2_2391 and Pf6N2E2_2392 are separated by 132 nucleotides Pf6N2E2_2392 and Pf6N2E2_2393 are separated by 103 nucleotides
Pf6N2E2_2390 - FIG00959101: hypothetical protein, at 2,829,557 to 2,829,763
_2390
Pf6N2E2_2391 - FIG137360: hypothetical protein, at 2,829,783 to 2,830,601
_2391
Pf6N2E2_2392 - ThiJ/PfpI family protein, at 2,830,734 to 2,831,579
_2392
Pf6N2E2_2393 - Transcriptional regulator, LysR family, at 2,831,683 to 2,832,579
_2393
Position (kb)
2830
2831
2832 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2829.761 kb on + strand at 2829.761 kb on + strand at 2829.762 kb on - strand at 2829.762 kb on - strand at 2829.824 kb on + strand at 2829.873 kb on - strand, within Pf6N2E2_2391 at 2829.889 kb on + strand, within Pf6N2E2_2391 at 2829.897 kb on + strand, within Pf6N2E2_2391 at 2829.897 kb on + strand, within Pf6N2E2_2391 at 2829.931 kb on + strand, within Pf6N2E2_2391 at 2829.932 kb on - strand, within Pf6N2E2_2391 at 2829.932 kb on - strand, within Pf6N2E2_2391 at 2829.932 kb on - strand, within Pf6N2E2_2391 at 2829.944 kb on + strand, within Pf6N2E2_2391 at 2829.945 kb on - strand, within Pf6N2E2_2391 at 2829.977 kb on + strand, within Pf6N2E2_2391 at 2829.978 kb on - strand, within Pf6N2E2_2391 at 2830.101 kb on + strand, within Pf6N2E2_2391 at 2830.143 kb on + strand, within Pf6N2E2_2391 at 2830.143 kb on + strand, within Pf6N2E2_2391 at 2830.143 kb on + strand, within Pf6N2E2_2391 at 2830.143 kb on + strand, within Pf6N2E2_2391 at 2830.143 kb on + strand, within Pf6N2E2_2391 at 2830.143 kb on + strand, within Pf6N2E2_2391 at 2830.144 kb on - strand, within Pf6N2E2_2391 at 2830.144 kb on - strand, within Pf6N2E2_2391 at 2830.144 kb on - strand, within Pf6N2E2_2391 at 2830.144 kb on - strand at 2830.206 kb on + strand, within Pf6N2E2_2391 at 2830.206 kb on + strand, within Pf6N2E2_2391 at 2830.209 kb on + strand, within Pf6N2E2_2391 at 2830.210 kb on - strand, within Pf6N2E2_2391 at 2830.210 kb on - strand, within Pf6N2E2_2391 at 2830.210 kb on - strand, within Pf6N2E2_2391 at 2830.231 kb on + strand, within Pf6N2E2_2391 at 2830.232 kb on - strand, within Pf6N2E2_2391 at 2830.232 kb on - strand, within Pf6N2E2_2391 at 2830.253 kb on + strand, within Pf6N2E2_2391 at 2830.272 kb on + strand, within Pf6N2E2_2391 at 2830.272 kb on + strand, within Pf6N2E2_2391 at 2830.273 kb on - strand, within Pf6N2E2_2391 at 2830.308 kb on + strand, within Pf6N2E2_2391 at 2830.308 kb on + strand, within Pf6N2E2_2391 at 2830.309 kb on - strand, within Pf6N2E2_2391 at 2830.455 kb on + strand, within Pf6N2E2_2391 at 2830.455 kb on + strand, within Pf6N2E2_2391 at 2830.455 kb on + strand, within Pf6N2E2_2391 at 2830.455 kb on + strand, within Pf6N2E2_2391 at 2830.455 kb on + strand, within Pf6N2E2_2391 at 2830.456 kb on - strand, within Pf6N2E2_2391 at 2830.512 kb on - strand, within Pf6N2E2_2391 at 2830.512 kb on - strand, within Pf6N2E2_2391 at 2830.512 kb on - strand, within Pf6N2E2_2391 at 2830.599 kb on + strand at 2830.736 kb on - strand at 2830.975 kb on + strand, within Pf6N2E2_2392 at 2830.975 kb on + strand, within Pf6N2E2_2392 at 2830.975 kb on + strand, within Pf6N2E2_2392 at 2830.975 kb on + strand, within Pf6N2E2_2392 at 2830.975 kb on + strand, within Pf6N2E2_2392 at 2830.976 kb on - strand, within Pf6N2E2_2392 at 2830.976 kb on - strand, within Pf6N2E2_2392 at 2830.976 kb on - strand, within Pf6N2E2_2392 at 2830.976 kb on - strand, within Pf6N2E2_2392 at 2831.182 kb on + strand, within Pf6N2E2_2392 at 2831.182 kb on + strand, within Pf6N2E2_2392 at 2831.182 kb on + strand, within Pf6N2E2_2392 at 2831.182 kb on + strand, within Pf6N2E2_2392 at 2831.182 kb on + strand, within Pf6N2E2_2392 at 2831.182 kb on + strand, within Pf6N2E2_2392 at 2831.183 kb on - strand, within Pf6N2E2_2392 at 2831.183 kb on - strand, within Pf6N2E2_2392 at 2831.262 kb on - strand, within Pf6N2E2_2392 at 2831.287 kb on + strand, within Pf6N2E2_2392 at 2831.320 kb on + strand, within Pf6N2E2_2392 at 2831.320 kb on + strand, within Pf6N2E2_2392 at 2831.320 kb on + strand, within Pf6N2E2_2392 at 2831.320 kb on + strand, within Pf6N2E2_2392 at 2831.321 kb on - strand, within Pf6N2E2_2392 at 2831.437 kb on + strand, within Pf6N2E2_2392 at 2831.619 kb on + strand at 2831.619 kb on + strand at 2831.702 kb on - strand at 2831.888 kb on - strand, within Pf6N2E2_2393 at 2831.888 kb on - strand, within Pf6N2E2_2393 at 2831.998 kb on + strand, within Pf6N2E2_2393 at 2831.998 kb on + strand, within Pf6N2E2_2393 at 2831.998 kb on + strand, within Pf6N2E2_2393 at 2831.998 kb on + strand, within Pf6N2E2_2393 at 2831.998 kb on + strand, within Pf6N2E2_2393 at 2831.999 kb on - strand, within Pf6N2E2_2393 at 2831.999 kb on - strand, within Pf6N2E2_2393 at 2831.999 kb on - strand, within Pf6N2E2_2393 at 2831.999 kb on - strand, within Pf6N2E2_2393 at 2831.999 kb on - strand, within Pf6N2E2_2393 at 2832.073 kb on - strand, within Pf6N2E2_2393 at 2832.371 kb on + strand, within Pf6N2E2_2393 at 2832.371 kb on + strand, within Pf6N2E2_2393 at 2832.371 kb on + strand, within Pf6N2E2_2393 at 2832.371 kb on + strand, within Pf6N2E2_2393 at 2832.371 kb on + strand, within Pf6N2E2_2393 at 2832.371 kb on + strand, within Pf6N2E2_2393 at 2832.371 kb on + strand, within Pf6N2E2_2393 at 2832.372 kb on - strand, within Pf6N2E2_2393 at 2832.372 kb on - strand, within Pf6N2E2_2393 at 2832.372 kb on - strand, within Pf6N2E2_2393 at 2832.372 kb on - strand, within Pf6N2E2_2393 at 2832.372 kb on - strand, within Pf6N2E2_2393 at 2832.372 kb on - strand, within Pf6N2E2_2393 at 2832.372 kb on - strand, within Pf6N2E2_2393 at 2832.372 kb on - strand, within Pf6N2E2_2393 at 2832.372 kb on - strand, within Pf6N2E2_2393 at 2832.421 kb on + strand, within Pf6N2E2_2393 at 2832.421 kb on + strand, within Pf6N2E2_2393 at 2832.421 kb on + strand, within Pf6N2E2_2393 at 2832.421 kb on + strand, within Pf6N2E2_2393 at 2832.421 kb on + strand, within Pf6N2E2_2393 at 2832.422 kb on - strand, within Pf6N2E2_2393 at 2832.422 kb on - strand, within Pf6N2E2_2393 at 2832.422 kb on - strand, within Pf6N2E2_2393
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,829,761 + +1.1 2,829,761 + +0.2 2,829,762 - -0.8 2,829,762 - +0.0 2,829,824 + +0.6 2,829,873 - Pf6N2E2_2391 0.11 -1.3 2,829,889 + Pf6N2E2_2391 0.13 -1.2 2,829,897 + Pf6N2E2_2391 0.14 +2.3 2,829,897 + Pf6N2E2_2391 0.14 +0.2 2,829,931 + Pf6N2E2_2391 0.18 +0.2 2,829,932 - Pf6N2E2_2391 0.18 -1.3 2,829,932 - Pf6N2E2_2391 0.18 -1.1 2,829,932 - Pf6N2E2_2391 0.18 -3.7 2,829,944 + Pf6N2E2_2391 0.20 +1.3 2,829,945 - Pf6N2E2_2391 0.20 -2.2 2,829,977 + Pf6N2E2_2391 0.24 -1.5 2,829,978 - Pf6N2E2_2391 0.24 -2.5 2,830,101 + Pf6N2E2_2391 0.39 +0.1 2,830,143 + Pf6N2E2_2391 0.44 -0.1 2,830,143 + Pf6N2E2_2391 0.44 +0.5 2,830,143 + Pf6N2E2_2391 0.44 +0.4 2,830,143 + Pf6N2E2_2391 0.44 -1.6 2,830,143 + Pf6N2E2_2391 0.44 +1.6 2,830,143 + Pf6N2E2_2391 0.44 +0.9 2,830,144 - Pf6N2E2_2391 0.44 -1.8 2,830,144 - Pf6N2E2_2391 0.44 -2.8 2,830,144 - Pf6N2E2_2391 0.44 -1.8 2,830,144 - -1.2 2,830,206 + Pf6N2E2_2391 0.52 +0.1 2,830,206 + Pf6N2E2_2391 0.52 -0.4 2,830,209 + Pf6N2E2_2391 0.52 -1.0 2,830,210 - Pf6N2E2_2391 0.52 -0.7 2,830,210 - Pf6N2E2_2391 0.52 -1.6 2,830,210 - Pf6N2E2_2391 0.52 -2.1 2,830,231 + Pf6N2E2_2391 0.55 -0.0 2,830,232 - Pf6N2E2_2391 0.55 -0.9 2,830,232 - Pf6N2E2_2391 0.55 -0.5 2,830,253 + Pf6N2E2_2391 0.57 -0.9 2,830,272 + Pf6N2E2_2391 0.60 -1.2 2,830,272 + Pf6N2E2_2391 0.60 -0.3 2,830,273 - Pf6N2E2_2391 0.60 -0.4 2,830,308 + Pf6N2E2_2391 0.64 -0.2 2,830,308 + Pf6N2E2_2391 0.64 -0.2 2,830,309 - Pf6N2E2_2391 0.64 -2.0 2,830,455 + Pf6N2E2_2391 0.82 -1.1 2,830,455 + Pf6N2E2_2391 0.82 -1.8 2,830,455 + Pf6N2E2_2391 0.82 -1.1 2,830,455 + Pf6N2E2_2391 0.82 -0.9 2,830,455 + Pf6N2E2_2391 0.82 -0.2 2,830,456 - Pf6N2E2_2391 0.82 -0.8 2,830,512 - Pf6N2E2_2391 0.89 -1.1 2,830,512 - Pf6N2E2_2391 0.89 -0.5 2,830,512 - Pf6N2E2_2391 0.89 +1.6 2,830,599 + -0.8 2,830,736 - -0.3 2,830,975 + Pf6N2E2_2392 0.28 +1.4 2,830,975 + Pf6N2E2_2392 0.28 -0.1 2,830,975 + Pf6N2E2_2392 0.28 +0.5 2,830,975 + Pf6N2E2_2392 0.28 +0.1 2,830,975 + Pf6N2E2_2392 0.28 -2.6 2,830,976 - Pf6N2E2_2392 0.29 +0.8 2,830,976 - Pf6N2E2_2392 0.29 +0.5 2,830,976 - Pf6N2E2_2392 0.29 +0.8 2,830,976 - Pf6N2E2_2392 0.29 +0.7 2,831,182 + Pf6N2E2_2392 0.53 -0.1 2,831,182 + Pf6N2E2_2392 0.53 +0.6 2,831,182 + Pf6N2E2_2392 0.53 +2.0 2,831,182 + Pf6N2E2_2392 0.53 -0.2 2,831,182 + Pf6N2E2_2392 0.53 +0.6 2,831,182 + Pf6N2E2_2392 0.53 +0.8 2,831,183 - Pf6N2E2_2392 0.53 -1.6 2,831,183 - Pf6N2E2_2392 0.53 -1.4 2,831,262 - Pf6N2E2_2392 0.62 +0.7 2,831,287 + Pf6N2E2_2392 0.65 +0.8 2,831,320 + Pf6N2E2_2392 0.69 -0.2 2,831,320 + Pf6N2E2_2392 0.69 -1.5 2,831,320 + Pf6N2E2_2392 0.69 +1.1 2,831,320 + Pf6N2E2_2392 0.69 -2.3 2,831,321 - Pf6N2E2_2392 0.69 +1.0 2,831,437 + Pf6N2E2_2392 0.83 +1.1 2,831,619 + -0.2 2,831,619 + -1.6 2,831,702 - -2.1 2,831,888 - Pf6N2E2_2393 0.23 +0.4 2,831,888 - Pf6N2E2_2393 0.23 -1.6 2,831,998 + Pf6N2E2_2393 0.35 +0.8 2,831,998 + Pf6N2E2_2393 0.35 +0.8 2,831,998 + Pf6N2E2_2393 0.35 +0.1 2,831,998 + Pf6N2E2_2393 0.35 -0.0 2,831,998 + Pf6N2E2_2393 0.35 -0.8 2,831,999 - Pf6N2E2_2393 0.35 +1.3 2,831,999 - Pf6N2E2_2393 0.35 +1.2 2,831,999 - Pf6N2E2_2393 0.35 -0.0 2,831,999 - Pf6N2E2_2393 0.35 +1.2 2,831,999 - Pf6N2E2_2393 0.35 +0.1 2,832,073 - Pf6N2E2_2393 0.43 +0.6 2,832,371 + Pf6N2E2_2393 0.77 +0.7 2,832,371 + Pf6N2E2_2393 0.77 +1.0 2,832,371 + Pf6N2E2_2393 0.77 -0.6 2,832,371 + Pf6N2E2_2393 0.77 +1.6 2,832,371 + Pf6N2E2_2393 0.77 +0.1 2,832,371 + Pf6N2E2_2393 0.77 +0.8 2,832,371 + Pf6N2E2_2393 0.77 +0.0 2,832,372 - Pf6N2E2_2393 0.77 -2.1 2,832,372 - Pf6N2E2_2393 0.77 -0.9 2,832,372 - Pf6N2E2_2393 0.77 +0.5 2,832,372 - Pf6N2E2_2393 0.77 +2.0 2,832,372 - Pf6N2E2_2393 0.77 +0.2 2,832,372 - Pf6N2E2_2393 0.77 +1.4 2,832,372 - Pf6N2E2_2393 0.77 +1.4 2,832,372 - Pf6N2E2_2393 0.77 -0.0 2,832,372 - Pf6N2E2_2393 0.77 -2.3 2,832,421 + Pf6N2E2_2393 0.82 +0.3 2,832,421 + Pf6N2E2_2393 0.82 -0.9 2,832,421 + Pf6N2E2_2393 0.82 +0.3 2,832,421 + Pf6N2E2_2393 0.82 +0.6 2,832,421 + Pf6N2E2_2393 0.82 -0.3 2,832,422 - Pf6N2E2_2393 0.82 +0.6 2,832,422 - Pf6N2E2_2393 0.82 -0.3 2,832,422 - Pf6N2E2_2393 0.82 -0.6
Or see this region's nucleotide sequence