Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2239 and Pf6N2E2_2240 are separated by 204 nucleotides Pf6N2E2_2240 and Pf6N2E2_2241 are separated by 178 nucleotides
Pf6N2E2_2239 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 2,653,256 to 2,654,827
_2239
Pf6N2E2_2240 - Ribonuclease T2 family protein, at 2,655,032 to 2,655,691
_2240
Pf6N2E2_2241 - Chromate transport protein ChrA, at 2,655,870 to 2,657,228
_2241
Position (kb)
2655
2656 Strain fitness (log2 ratio)
-1
0
1
2 at 2654.098 kb on + strand, within Pf6N2E2_2239 at 2654.232 kb on + strand, within Pf6N2E2_2239 at 2654.350 kb on + strand, within Pf6N2E2_2239 at 2654.386 kb on + strand, within Pf6N2E2_2239 at 2654.386 kb on + strand, within Pf6N2E2_2239 at 2654.386 kb on + strand, within Pf6N2E2_2239 at 2654.387 kb on - strand, within Pf6N2E2_2239 at 2654.387 kb on - strand, within Pf6N2E2_2239 at 2654.391 kb on + strand, within Pf6N2E2_2239 at 2654.392 kb on - strand, within Pf6N2E2_2239 at 2654.392 kb on - strand, within Pf6N2E2_2239 at 2654.392 kb on - strand, within Pf6N2E2_2239 at 2654.706 kb on + strand at 2654.707 kb on - strand at 2654.815 kb on + strand at 2654.891 kb on + strand at 2654.892 kb on - strand at 2655.234 kb on + strand, within Pf6N2E2_2240 at 2655.234 kb on + strand, within Pf6N2E2_2240 at 2655.237 kb on + strand, within Pf6N2E2_2240 at 2655.238 kb on - strand, within Pf6N2E2_2240 at 2655.238 kb on - strand, within Pf6N2E2_2240 at 2655.238 kb on - strand, within Pf6N2E2_2240 at 2655.309 kb on + strand, within Pf6N2E2_2240 at 2655.310 kb on - strand, within Pf6N2E2_2240 at 2655.463 kb on - strand, within Pf6N2E2_2240 at 2655.463 kb on - strand, within Pf6N2E2_2240 at 2655.570 kb on + strand, within Pf6N2E2_2240 at 2655.571 kb on - strand, within Pf6N2E2_2240 at 2655.571 kb on - strand, within Pf6N2E2_2240 at 2655.571 kb on - strand at 2655.571 kb on - strand, within Pf6N2E2_2240 at 2655.571 kb on - strand, within Pf6N2E2_2240 at 2655.571 kb on - strand, within Pf6N2E2_2240 at 2655.574 kb on - strand, within Pf6N2E2_2240 at 2655.574 kb on - strand, within Pf6N2E2_2240 at 2655.657 kb on - strand at 2655.785 kb on - strand at 2655.811 kb on + strand at 2655.811 kb on + strand at 2655.812 kb on - strand at 2655.812 kb on - strand at 2655.835 kb on + strand at 2655.835 kb on + strand at 2655.835 kb on + strand at 2655.835 kb on + strand at 2655.835 kb on + strand at 2655.835 kb on + strand at 2655.835 kb on + strand at 2655.835 kb on + strand at 2655.835 kb on + strand at 2655.836 kb on - strand at 2655.836 kb on - strand at 2655.836 kb on - strand at 2655.836 kb on - strand at 2655.836 kb on - strand at 2655.836 kb on - strand at 2655.836 kb on - strand at 2655.836 kb on - strand at 2655.836 kb on - strand at 2655.836 kb on - strand at 2655.836 kb on - strand at 2655.853 kb on + strand at 2655.853 kb on + strand at 2655.854 kb on - strand at 2655.961 kb on + strand at 2655.961 kb on + strand at 2655.961 kb on + strand at 2655.962 kb on - strand at 2656.071 kb on + strand, within Pf6N2E2_2241 at 2656.071 kb on + strand, within Pf6N2E2_2241 at 2656.071 kb on + strand, within Pf6N2E2_2241 at 2656.071 kb on + strand, within Pf6N2E2_2241 at 2656.116 kb on + strand, within Pf6N2E2_2241 at 2656.116 kb on + strand, within Pf6N2E2_2241 at 2656.116 kb on + strand, within Pf6N2E2_2241 at 2656.116 kb on + strand, within Pf6N2E2_2241 at 2656.116 kb on + strand, within Pf6N2E2_2241 at 2656.116 kb on + strand, within Pf6N2E2_2241 at 2656.116 kb on + strand, within Pf6N2E2_2241 at 2656.116 kb on + strand, within Pf6N2E2_2241 at 2656.116 kb on + strand, within Pf6N2E2_2241 at 2656.116 kb on + strand, within Pf6N2E2_2241 at 2656.117 kb on - strand, within Pf6N2E2_2241 at 2656.117 kb on - strand, within Pf6N2E2_2241 at 2656.117 kb on - strand, within Pf6N2E2_2241 at 2656.117 kb on - strand, within Pf6N2E2_2241 at 2656.117 kb on - strand, within Pf6N2E2_2241 at 2656.215 kb on + strand, within Pf6N2E2_2241 at 2656.215 kb on + strand, within Pf6N2E2_2241 at 2656.215 kb on + strand, within Pf6N2E2_2241 at 2656.216 kb on - strand, within Pf6N2E2_2241 at 2656.216 kb on - strand, within Pf6N2E2_2241 at 2656.216 kb on - strand, within Pf6N2E2_2241 at 2656.372 kb on + strand, within Pf6N2E2_2241 at 2656.373 kb on - strand, within Pf6N2E2_2241 at 2656.373 kb on - strand, within Pf6N2E2_2241
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,654,098 + Pf6N2E2_2239 0.54 +0.1 2,654,232 + Pf6N2E2_2239 0.62 -0.7 2,654,350 + Pf6N2E2_2239 0.70 -0.7 2,654,386 + Pf6N2E2_2239 0.72 +0.9 2,654,386 + Pf6N2E2_2239 0.72 -0.7 2,654,386 + Pf6N2E2_2239 0.72 +0.6 2,654,387 - Pf6N2E2_2239 0.72 -1.0 2,654,387 - Pf6N2E2_2239 0.72 -0.7 2,654,391 + Pf6N2E2_2239 0.72 -0.5 2,654,392 - Pf6N2E2_2239 0.72 +1.0 2,654,392 - Pf6N2E2_2239 0.72 +0.2 2,654,392 - Pf6N2E2_2239 0.72 +0.3 2,654,706 + -0.2 2,654,707 - -0.0 2,654,815 + -1.2 2,654,891 + +1.5 2,654,892 - -0.2 2,655,234 + Pf6N2E2_2240 0.31 -0.3 2,655,234 + Pf6N2E2_2240 0.31 +1.0 2,655,237 + Pf6N2E2_2240 0.31 +0.9 2,655,238 - Pf6N2E2_2240 0.31 +1.9 2,655,238 - Pf6N2E2_2240 0.31 +1.8 2,655,238 - Pf6N2E2_2240 0.31 +0.7 2,655,309 + Pf6N2E2_2240 0.42 +0.4 2,655,310 - Pf6N2E2_2240 0.42 +0.8 2,655,463 - Pf6N2E2_2240 0.65 +0.5 2,655,463 - Pf6N2E2_2240 0.65 +1.2 2,655,570 + Pf6N2E2_2240 0.82 +2.1 2,655,571 - Pf6N2E2_2240 0.82 +0.2 2,655,571 - Pf6N2E2_2240 0.82 +1.2 2,655,571 - +0.7 2,655,571 - Pf6N2E2_2240 0.82 +1.5 2,655,571 - Pf6N2E2_2240 0.82 +0.1 2,655,571 - Pf6N2E2_2240 0.82 -0.5 2,655,574 - Pf6N2E2_2240 0.82 +2.0 2,655,574 - Pf6N2E2_2240 0.82 +0.1 2,655,657 - +1.9 2,655,785 - +0.8 2,655,811 + +0.5 2,655,811 + +0.3 2,655,812 - +0.0 2,655,812 - +0.7 2,655,835 + -1.6 2,655,835 + +0.5 2,655,835 + +0.2 2,655,835 + +0.3 2,655,835 + -0.6 2,655,835 + -0.0 2,655,835 + +0.3 2,655,835 + -0.6 2,655,835 + +2.2 2,655,836 - -0.7 2,655,836 - -0.8 2,655,836 - +0.6 2,655,836 - +0.2 2,655,836 - +1.7 2,655,836 - +0.0 2,655,836 - +0.1 2,655,836 - -0.0 2,655,836 - +0.4 2,655,836 - -0.6 2,655,836 - +0.2 2,655,853 + +1.4 2,655,853 + -0.4 2,655,854 - -0.5 2,655,961 + +0.9 2,655,961 + +1.1 2,655,961 + -0.1 2,655,962 - +0.7 2,656,071 + Pf6N2E2_2241 0.15 -0.1 2,656,071 + Pf6N2E2_2241 0.15 +1.4 2,656,071 + Pf6N2E2_2241 0.15 +0.6 2,656,071 + Pf6N2E2_2241 0.15 +1.1 2,656,116 + Pf6N2E2_2241 0.18 +0.7 2,656,116 + Pf6N2E2_2241 0.18 -0.7 2,656,116 + Pf6N2E2_2241 0.18 +1.8 2,656,116 + Pf6N2E2_2241 0.18 +0.6 2,656,116 + Pf6N2E2_2241 0.18 -0.3 2,656,116 + Pf6N2E2_2241 0.18 +0.6 2,656,116 + Pf6N2E2_2241 0.18 -0.4 2,656,116 + Pf6N2E2_2241 0.18 -0.4 2,656,116 + Pf6N2E2_2241 0.18 +1.0 2,656,116 + Pf6N2E2_2241 0.18 +0.9 2,656,117 - Pf6N2E2_2241 0.18 +0.6 2,656,117 - Pf6N2E2_2241 0.18 -0.5 2,656,117 - Pf6N2E2_2241 0.18 -1.0 2,656,117 - Pf6N2E2_2241 0.18 -0.5 2,656,117 - Pf6N2E2_2241 0.18 -1.7 2,656,215 + Pf6N2E2_2241 0.25 +1.7 2,656,215 + Pf6N2E2_2241 0.25 +0.5 2,656,215 + Pf6N2E2_2241 0.25 -1.0 2,656,216 - Pf6N2E2_2241 0.25 -1.5 2,656,216 - Pf6N2E2_2241 0.25 +0.7 2,656,216 - Pf6N2E2_2241 0.25 +0.2 2,656,372 + Pf6N2E2_2241 0.37 -1.3 2,656,373 - Pf6N2E2_2241 0.37 +0.5 2,656,373 - Pf6N2E2_2241 0.37 -0.5
Or see this region's nucleotide sequence