Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1348 and Pf6N2E2_1349 are separated by 77 nucleotides Pf6N2E2_1349 and Pf6N2E2_1350 overlap by 38 nucleotides Pf6N2E2_1350 and Pf6N2E2_1351 overlap by 4 nucleotides Pf6N2E2_1351 and Pf6N2E2_1352 are separated by 21 nucleotides Pf6N2E2_1352 and Pf6N2E2_1353 overlap by 4 nucleotides
Pf6N2E2_1348 - COG2907: Amine oxidase, flavin-containing, at 1,583,539 to 1,584,786
_1348
Pf6N2E2_1349 - FIG001571: Hypothetical protein, at 1,584,864 to 1,585,607
_1349
Pf6N2E2_1350 - Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79), plant type, at 1,585,570 to 1,586,838
_1350
Pf6N2E2_1351 - FIG024285: Hypothetical protein, at 1,586,835 to 1,587,326
_1351
Pf6N2E2_1352 - Cation transport ATPase, at 1,587,348 to 1,587,653
_1352
Pf6N2E2_1353 - FIG002577: Putative lipoprotein precursor, at 1,587,650 to 1,588,189
_1353
Position (kb)
1585
1586
1587 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1584.616 kb on + strand, within Pf6N2E2_1348 at 1584.616 kb on + strand, within Pf6N2E2_1348 at 1584.632 kb on - strand, within Pf6N2E2_1348 at 1584.694 kb on + strand at 1584.694 kb on + strand at 1584.694 kb on + strand at 1584.695 kb on - strand at 1584.695 kb on - strand at 1584.695 kb on - strand at 1584.695 kb on - strand at 1584.695 kb on - strand at 1584.788 kb on + strand at 1584.788 kb on + strand at 1584.789 kb on - strand at 1584.827 kb on + strand at 1584.828 kb on - strand at 1584.870 kb on + strand at 1584.870 kb on + strand at 1584.870 kb on + strand at 1584.871 kb on - strand at 1584.871 kb on - strand at 1584.871 kb on - strand at 1585.113 kb on + strand, within Pf6N2E2_1349 at 1585.113 kb on + strand, within Pf6N2E2_1349 at 1585.113 kb on + strand, within Pf6N2E2_1349 at 1585.194 kb on + strand, within Pf6N2E2_1349 at 1585.194 kb on + strand, within Pf6N2E2_1349 at 1585.194 kb on + strand, within Pf6N2E2_1349 at 1585.194 kb on + strand, within Pf6N2E2_1349 at 1585.194 kb on + strand, within Pf6N2E2_1349 at 1585.194 kb on + strand, within Pf6N2E2_1349 at 1585.194 kb on + strand, within Pf6N2E2_1349 at 1585.195 kb on - strand, within Pf6N2E2_1349 at 1585.195 kb on - strand, within Pf6N2E2_1349 at 1585.195 kb on - strand, within Pf6N2E2_1349 at 1585.195 kb on - strand, within Pf6N2E2_1349 at 1585.195 kb on - strand, within Pf6N2E2_1349 at 1585.198 kb on + strand, within Pf6N2E2_1349 at 1585.198 kb on + strand, within Pf6N2E2_1349 at 1585.198 kb on + strand, within Pf6N2E2_1349 at 1585.198 kb on + strand, within Pf6N2E2_1349 at 1585.199 kb on - strand, within Pf6N2E2_1349 at 1585.199 kb on - strand, within Pf6N2E2_1349 at 1585.199 kb on - strand at 1585.284 kb on + strand, within Pf6N2E2_1349 at 1585.284 kb on + strand, within Pf6N2E2_1349 at 1585.285 kb on - strand, within Pf6N2E2_1349 at 1585.324 kb on + strand, within Pf6N2E2_1349 at 1585.325 kb on - strand, within Pf6N2E2_1349 at 1585.465 kb on - strand, within Pf6N2E2_1349 at 1585.506 kb on + strand, within Pf6N2E2_1349 at 1585.507 kb on - strand, within Pf6N2E2_1349 at 1585.554 kb on + strand at 1585.554 kb on + strand at 1585.555 kb on - strand at 1585.555 kb on - strand at 1585.555 kb on - strand at 1585.556 kb on + strand at 1585.556 kb on + strand at 1585.556 kb on + strand at 1585.556 kb on + strand at 1585.556 kb on + strand at 1585.556 kb on + strand at 1585.556 kb on + strand at 1585.557 kb on - strand at 1585.557 kb on - strand at 1585.557 kb on - strand at 1585.557 kb on - strand at 1585.557 kb on - strand at 1585.557 kb on - strand at 1585.557 kb on - strand at 1585.557 kb on - strand at 1585.606 kb on - strand at 1585.609 kb on + strand at 1585.609 kb on + strand at 1585.610 kb on - strand at 1585.691 kb on + strand at 1585.816 kb on + strand, within Pf6N2E2_1350 at 1585.816 kb on + strand, within Pf6N2E2_1350 at 1585.816 kb on + strand, within Pf6N2E2_1350 at 1585.817 kb on - strand, within Pf6N2E2_1350 at 1585.817 kb on - strand, within Pf6N2E2_1350 at 1585.817 kb on - strand, within Pf6N2E2_1350 at 1585.817 kb on - strand, within Pf6N2E2_1350 at 1585.817 kb on - strand, within Pf6N2E2_1350 at 1585.987 kb on - strand, within Pf6N2E2_1350 at 1585.999 kb on + strand, within Pf6N2E2_1350 at 1586.000 kb on - strand, within Pf6N2E2_1350 at 1586.000 kb on - strand, within Pf6N2E2_1350 at 1586.050 kb on + strand, within Pf6N2E2_1350 at 1586.051 kb on - strand, within Pf6N2E2_1350 at 1586.119 kb on - strand, within Pf6N2E2_1350 at 1586.195 kb on - strand, within Pf6N2E2_1350 at 1586.252 kb on - strand, within Pf6N2E2_1350 at 1586.252 kb on - strand, within Pf6N2E2_1350 at 1586.252 kb on - strand, within Pf6N2E2_1350 at 1586.252 kb on - strand, within Pf6N2E2_1350 at 1586.252 kb on - strand, within Pf6N2E2_1350 at 1586.252 kb on - strand, within Pf6N2E2_1350 at 1586.321 kb on - strand, within Pf6N2E2_1350 at 1586.389 kb on + strand, within Pf6N2E2_1350 at 1586.393 kb on + strand, within Pf6N2E2_1350 at 1586.393 kb on + strand, within Pf6N2E2_1350 at 1586.394 kb on - strand, within Pf6N2E2_1350 at 1586.402 kb on - strand, within Pf6N2E2_1350 at 1586.480 kb on - strand, within Pf6N2E2_1350 at 1586.725 kb on + strand at 1586.725 kb on + strand at 1586.725 kb on + strand at 1586.726 kb on - strand at 1586.726 kb on - strand at 1586.833 kb on - strand at 1586.926 kb on + strand, within Pf6N2E2_1351 at 1586.927 kb on - strand, within Pf6N2E2_1351 at 1586.927 kb on - strand, within Pf6N2E2_1351 at 1586.929 kb on + strand, within Pf6N2E2_1351 at 1586.929 kb on + strand, within Pf6N2E2_1351 at 1586.929 kb on + strand, within Pf6N2E2_1351 at 1586.930 kb on - strand, within Pf6N2E2_1351 at 1586.930 kb on - strand, within Pf6N2E2_1351 at 1586.930 kb on - strand, within Pf6N2E2_1351 at 1587.061 kb on + strand, within Pf6N2E2_1351 at 1587.061 kb on + strand, within Pf6N2E2_1351 at 1587.061 kb on + strand, within Pf6N2E2_1351 at 1587.062 kb on - strand, within Pf6N2E2_1351 at 1587.062 kb on - strand, within Pf6N2E2_1351 at 1587.131 kb on + strand, within Pf6N2E2_1351 at 1587.169 kb on + strand, within Pf6N2E2_1351 at 1587.169 kb on + strand, within Pf6N2E2_1351 at 1587.169 kb on + strand, within Pf6N2E2_1351 at 1587.201 kb on + strand, within Pf6N2E2_1351 at 1587.201 kb on + strand, within Pf6N2E2_1351 at 1587.201 kb on + strand, within Pf6N2E2_1351 at 1587.568 kb on - strand, within Pf6N2E2_1352 at 1587.568 kb on - strand, within Pf6N2E2_1352 at 1587.572 kb on - strand, within Pf6N2E2_1352 at 1587.572 kb on - strand, within Pf6N2E2_1352 at 1587.698 kb on + strand at 1587.760 kb on - strand, within Pf6N2E2_1353 at 1587.760 kb on - strand, within Pf6N2E2_1353 at 1587.786 kb on + strand, within Pf6N2E2_1353 at 1587.786 kb on + strand, within Pf6N2E2_1353 at 1587.786 kb on + strand, within Pf6N2E2_1353 at 1587.787 kb on - strand, within Pf6N2E2_1353
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,584,616 + Pf6N2E2_1348 0.86 +1.2 1,584,616 + Pf6N2E2_1348 0.86 +0.1 1,584,632 - Pf6N2E2_1348 0.88 -1.4 1,584,694 + +3.1 1,584,694 + +0.6 1,584,694 + +1.5 1,584,695 - +0.3 1,584,695 - -1.6 1,584,695 - -1.8 1,584,695 - -0.5 1,584,695 - +0.2 1,584,788 + +0.5 1,584,788 + -0.2 1,584,789 - -1.1 1,584,827 + -0.9 1,584,828 - +0.2 1,584,870 + -0.4 1,584,870 + +0.5 1,584,870 + -0.3 1,584,871 - +1.1 1,584,871 - -1.7 1,584,871 - +0.3 1,585,113 + Pf6N2E2_1349 0.33 +1.4 1,585,113 + Pf6N2E2_1349 0.33 -0.0 1,585,113 + Pf6N2E2_1349 0.33 +0.0 1,585,194 + Pf6N2E2_1349 0.44 +0.7 1,585,194 + Pf6N2E2_1349 0.44 +0.8 1,585,194 + Pf6N2E2_1349 0.44 -1.0 1,585,194 + Pf6N2E2_1349 0.44 +1.5 1,585,194 + Pf6N2E2_1349 0.44 +0.2 1,585,194 + Pf6N2E2_1349 0.44 +1.2 1,585,194 + Pf6N2E2_1349 0.44 +0.9 1,585,195 - Pf6N2E2_1349 0.44 -1.0 1,585,195 - Pf6N2E2_1349 0.44 -0.5 1,585,195 - Pf6N2E2_1349 0.44 -2.7 1,585,195 - Pf6N2E2_1349 0.44 -0.1 1,585,195 - Pf6N2E2_1349 0.44 -0.7 1,585,198 + Pf6N2E2_1349 0.45 +0.6 1,585,198 + Pf6N2E2_1349 0.45 +0.6 1,585,198 + Pf6N2E2_1349 0.45 +1.0 1,585,198 + Pf6N2E2_1349 0.45 +0.3 1,585,199 - Pf6N2E2_1349 0.45 +0.0 1,585,199 - Pf6N2E2_1349 0.45 +1.0 1,585,199 - -0.1 1,585,284 + Pf6N2E2_1349 0.56 -1.5 1,585,284 + Pf6N2E2_1349 0.56 -0.4 1,585,285 - Pf6N2E2_1349 0.57 +0.5 1,585,324 + Pf6N2E2_1349 0.62 +0.1 1,585,325 - Pf6N2E2_1349 0.62 +0.6 1,585,465 - Pf6N2E2_1349 0.81 -0.1 1,585,506 + Pf6N2E2_1349 0.86 +0.0 1,585,507 - Pf6N2E2_1349 0.86 -0.5 1,585,554 + +1.0 1,585,554 + +0.3 1,585,555 - -2.6 1,585,555 - +0.3 1,585,555 - -0.5 1,585,556 + +0.3 1,585,556 + +1.0 1,585,556 + +0.1 1,585,556 + -0.3 1,585,556 + +1.3 1,585,556 + +1.1 1,585,556 + +2.3 1,585,557 - +0.7 1,585,557 - -1.0 1,585,557 - -1.0 1,585,557 - -0.2 1,585,557 - +1.1 1,585,557 - +0.3 1,585,557 - -0.9 1,585,557 - -1.5 1,585,606 - +2.9 1,585,609 + -0.8 1,585,609 + -1.0 1,585,610 - -0.9 1,585,691 + +2.1 1,585,816 + Pf6N2E2_1350 0.19 -0.2 1,585,816 + Pf6N2E2_1350 0.19 -0.5 1,585,816 + Pf6N2E2_1350 0.19 -0.5 1,585,817 - Pf6N2E2_1350 0.19 +1.9 1,585,817 - Pf6N2E2_1350 0.19 -0.9 1,585,817 - Pf6N2E2_1350 0.19 -0.3 1,585,817 - Pf6N2E2_1350 0.19 -0.1 1,585,817 - Pf6N2E2_1350 0.19 +0.5 1,585,987 - Pf6N2E2_1350 0.33 -2.0 1,585,999 + Pf6N2E2_1350 0.34 +0.7 1,586,000 - Pf6N2E2_1350 0.34 +0.1 1,586,000 - Pf6N2E2_1350 0.34 +0.4 1,586,050 + Pf6N2E2_1350 0.38 +1.4 1,586,051 - Pf6N2E2_1350 0.38 -0.6 1,586,119 - Pf6N2E2_1350 0.43 +0.1 1,586,195 - Pf6N2E2_1350 0.49 +0.7 1,586,252 - Pf6N2E2_1350 0.54 -0.7 1,586,252 - Pf6N2E2_1350 0.54 -1.0 1,586,252 - Pf6N2E2_1350 0.54 -0.5 1,586,252 - Pf6N2E2_1350 0.54 -0.9 1,586,252 - Pf6N2E2_1350 0.54 -2.1 1,586,252 - Pf6N2E2_1350 0.54 -0.6 1,586,321 - Pf6N2E2_1350 0.59 -1.2 1,586,389 + Pf6N2E2_1350 0.65 +1.3 1,586,393 + Pf6N2E2_1350 0.65 -0.7 1,586,393 + Pf6N2E2_1350 0.65 +1.2 1,586,394 - Pf6N2E2_1350 0.65 +0.8 1,586,402 - Pf6N2E2_1350 0.66 +1.5 1,586,480 - Pf6N2E2_1350 0.72 -0.2 1,586,725 + -0.6 1,586,725 + +0.9 1,586,725 + +0.8 1,586,726 - +0.0 1,586,726 - +0.3 1,586,833 - -0.9 1,586,926 + Pf6N2E2_1351 0.18 -0.4 1,586,927 - Pf6N2E2_1351 0.19 -0.9 1,586,927 - Pf6N2E2_1351 0.19 +0.7 1,586,929 + Pf6N2E2_1351 0.19 +1.5 1,586,929 + Pf6N2E2_1351 0.19 +0.0 1,586,929 + Pf6N2E2_1351 0.19 +0.1 1,586,930 - Pf6N2E2_1351 0.19 -0.5 1,586,930 - Pf6N2E2_1351 0.19 -2.3 1,586,930 - Pf6N2E2_1351 0.19 +0.5 1,587,061 + Pf6N2E2_1351 0.46 -0.8 1,587,061 + Pf6N2E2_1351 0.46 -1.0 1,587,061 + Pf6N2E2_1351 0.46 +1.3 1,587,062 - Pf6N2E2_1351 0.46 -0.3 1,587,062 - Pf6N2E2_1351 0.46 +2.4 1,587,131 + Pf6N2E2_1351 0.60 +1.0 1,587,169 + Pf6N2E2_1351 0.68 +0.1 1,587,169 + Pf6N2E2_1351 0.68 -2.0 1,587,169 + Pf6N2E2_1351 0.68 -0.7 1,587,201 + Pf6N2E2_1351 0.74 +0.5 1,587,201 + Pf6N2E2_1351 0.74 -1.6 1,587,201 + Pf6N2E2_1351 0.74 -0.9 1,587,568 - Pf6N2E2_1352 0.72 -0.2 1,587,568 - Pf6N2E2_1352 0.72 +0.3 1,587,572 - Pf6N2E2_1352 0.73 -0.7 1,587,572 - Pf6N2E2_1352 0.73 -0.9 1,587,698 + +0.8 1,587,760 - Pf6N2E2_1353 0.20 +0.6 1,587,760 - Pf6N2E2_1353 0.20 +2.4 1,587,786 + Pf6N2E2_1353 0.25 -0.3 1,587,786 + Pf6N2E2_1353 0.25 +1.6 1,587,786 + Pf6N2E2_1353 0.25 +0.3 1,587,787 - Pf6N2E2_1353 0.25 +0.8
Or see this region's nucleotide sequence