Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1143 and Pf6N2E2_1144 are separated by 16 nucleotides Pf6N2E2_1144 and Pf6N2E2_1145 are separated by 13 nucleotides
Pf6N2E2_1143 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases, at 1,339,620 to 1,341,293
_1143
Pf6N2E2_1144 - Probable acyl-CoA dehydrogenase (EC 1.3.99.3), at 1,341,310 to 1,342,071
_1144
Pf6N2E2_1145 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 1,342,085 to 1,343,275
_1145
Position (kb)
1341
1342
1343 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1340.365 kb on - strand, within Pf6N2E2_1143 at 1340.405 kb on + strand, within Pf6N2E2_1143 at 1340.405 kb on + strand, within Pf6N2E2_1143 at 1340.405 kb on + strand, within Pf6N2E2_1143 at 1340.412 kb on + strand, within Pf6N2E2_1143 at 1340.412 kb on + strand, within Pf6N2E2_1143 at 1340.412 kb on + strand, within Pf6N2E2_1143 at 1340.413 kb on - strand, within Pf6N2E2_1143 at 1340.413 kb on - strand, within Pf6N2E2_1143 at 1340.413 kb on - strand, within Pf6N2E2_1143 at 1340.413 kb on - strand, within Pf6N2E2_1143 at 1340.413 kb on - strand, within Pf6N2E2_1143 at 1340.460 kb on + strand, within Pf6N2E2_1143 at 1340.460 kb on + strand, within Pf6N2E2_1143 at 1340.461 kb on - strand, within Pf6N2E2_1143 at 1340.461 kb on - strand, within Pf6N2E2_1143 at 1340.656 kb on + strand, within Pf6N2E2_1143 at 1340.656 kb on + strand, within Pf6N2E2_1143 at 1340.656 kb on + strand, within Pf6N2E2_1143 at 1340.699 kb on - strand, within Pf6N2E2_1143 at 1340.874 kb on + strand, within Pf6N2E2_1143 at 1340.874 kb on + strand, within Pf6N2E2_1143 at 1340.875 kb on - strand, within Pf6N2E2_1143 at 1340.877 kb on + strand, within Pf6N2E2_1143 at 1340.877 kb on + strand, within Pf6N2E2_1143 at 1340.877 kb on + strand, within Pf6N2E2_1143 at 1340.877 kb on + strand, within Pf6N2E2_1143 at 1340.877 kb on + strand, within Pf6N2E2_1143 at 1340.877 kb on + strand, within Pf6N2E2_1143 at 1340.877 kb on + strand, within Pf6N2E2_1143 at 1340.878 kb on - strand, within Pf6N2E2_1143 at 1340.878 kb on - strand, within Pf6N2E2_1143 at 1340.878 kb on - strand, within Pf6N2E2_1143 at 1340.954 kb on + strand, within Pf6N2E2_1143 at 1340.955 kb on - strand, within Pf6N2E2_1143 at 1341.230 kb on + strand at 1341.231 kb on - strand at 1341.999 kb on + strand at 1341.999 kb on + strand at 1341.999 kb on + strand at 1342.000 kb on - strand at 1342.000 kb on - strand at 1342.057 kb on - strand at 1342.057 kb on - strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.070 kb on - strand at 1342.070 kb on - strand at 1342.070 kb on - strand at 1342.073 kb on + strand at 1342.074 kb on - strand at 1342.074 kb on - strand at 1342.074 kb on - strand at 1342.074 kb on - strand at 1342.102 kb on + strand at 1342.102 kb on + strand at 1342.102 kb on + strand at 1342.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,340,365 - Pf6N2E2_1143 0.45 -0.7 1,340,405 + Pf6N2E2_1143 0.47 +0.4 1,340,405 + Pf6N2E2_1143 0.47 -0.6 1,340,405 + Pf6N2E2_1143 0.47 +0.4 1,340,412 + Pf6N2E2_1143 0.47 -0.1 1,340,412 + Pf6N2E2_1143 0.47 -0.7 1,340,412 + Pf6N2E2_1143 0.47 -0.6 1,340,413 - Pf6N2E2_1143 0.47 -2.5 1,340,413 - Pf6N2E2_1143 0.47 -1.9 1,340,413 - Pf6N2E2_1143 0.47 -3.0 1,340,413 - Pf6N2E2_1143 0.47 +0.0 1,340,413 - Pf6N2E2_1143 0.47 -3.6 1,340,460 + Pf6N2E2_1143 0.50 +0.6 1,340,460 + Pf6N2E2_1143 0.50 -0.7 1,340,461 - Pf6N2E2_1143 0.50 -2.0 1,340,461 - Pf6N2E2_1143 0.50 -2.6 1,340,656 + Pf6N2E2_1143 0.62 +0.6 1,340,656 + Pf6N2E2_1143 0.62 +0.3 1,340,656 + Pf6N2E2_1143 0.62 +0.2 1,340,699 - Pf6N2E2_1143 0.64 -1.4 1,340,874 + Pf6N2E2_1143 0.75 -0.5 1,340,874 + Pf6N2E2_1143 0.75 +1.7 1,340,875 - Pf6N2E2_1143 0.75 -1.4 1,340,877 + Pf6N2E2_1143 0.75 -0.1 1,340,877 + Pf6N2E2_1143 0.75 +0.5 1,340,877 + Pf6N2E2_1143 0.75 +0.8 1,340,877 + Pf6N2E2_1143 0.75 +2.7 1,340,877 + Pf6N2E2_1143 0.75 -2.0 1,340,877 + Pf6N2E2_1143 0.75 +0.5 1,340,877 + Pf6N2E2_1143 0.75 +0.5 1,340,878 - Pf6N2E2_1143 0.75 -3.0 1,340,878 - Pf6N2E2_1143 0.75 -3.0 1,340,878 - Pf6N2E2_1143 0.75 -3.0 1,340,954 + Pf6N2E2_1143 0.80 +0.3 1,340,955 - Pf6N2E2_1143 0.80 +0.5 1,341,230 + +1.1 1,341,231 - -3.1 1,341,999 + -2.4 1,341,999 + -3.1 1,341,999 + +0.2 1,342,000 - -1.6 1,342,000 - -1.7 1,342,057 - -1.1 1,342,057 - -1.7 1,342,069 + +0.0 1,342,069 + +0.6 1,342,069 + -0.2 1,342,069 + +0.1 1,342,069 + +1.1 1,342,069 + +0.0 1,342,069 + +0.5 1,342,070 - -1.8 1,342,070 - -0.1 1,342,070 - -2.4 1,342,073 + -0.3 1,342,074 - -0.3 1,342,074 - -2.9 1,342,074 - -1.0 1,342,074 - -1.9 1,342,102 + -2.0 1,342,102 + -2.4 1,342,102 + -1.6 1,342,103 - -2.0
Or see this region's nucleotide sequence