Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_109 and Pf6N2E2_110 are separated by 188 nucleotides Pf6N2E2_110 and Pf6N2E2_111 overlap by 17 nucleotides
Pf6N2E2_109 - Hydrolases of the alpha/beta superfamily, at 154,615 to 155,490
_109
Pf6N2E2_110 - Cellobiose phosphotransferase system YdjC-like protein, at 155,679 to 156,461
_110
Pf6N2E2_111 - FIG00954746: hypothetical protein, at 156,445 to 157,620
_111
Position (kb) 155
156
157 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 154.731 kb on + strand, within Pf6N2E2_109 at 154.732 kb on - strand, within Pf6N2E2_109 at 154.920 kb on + strand, within Pf6N2E2_109 at 154.920 kb on + strand, within Pf6N2E2_109 at 154.920 kb on + strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 155.069 kb on + strand, within Pf6N2E2_109 at 155.070 kb on - strand, within Pf6N2E2_109 at 155.070 kb on - strand, within Pf6N2E2_109 at 155.070 kb on - strand, within Pf6N2E2_109 at 155.102 kb on - strand, within Pf6N2E2_109 at 155.138 kb on + strand, within Pf6N2E2_109 at 155.138 kb on + strand, within Pf6N2E2_109 at 155.138 kb on + strand, within Pf6N2E2_109 at 155.235 kb on + strand, within Pf6N2E2_109 at 155.279 kb on + strand, within Pf6N2E2_109 at 155.280 kb on - strand, within Pf6N2E2_109 at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.449 kb on - strand at 155.449 kb on - strand at 155.449 kb on - strand at 155.449 kb on - strand at 155.545 kb on + strand at 155.546 kb on - strand at 155.546 kb on - strand at 155.591 kb on - strand at 155.640 kb on + strand at 155.662 kb on + strand at 155.769 kb on + strand, within Pf6N2E2_110 at 155.769 kb on + strand, within Pf6N2E2_110 at 155.770 kb on - strand, within Pf6N2E2_110 at 155.860 kb on + strand, within Pf6N2E2_110 at 155.860 kb on + strand, within Pf6N2E2_110 at 155.861 kb on - strand, within Pf6N2E2_110 at 155.861 kb on - strand, within Pf6N2E2_110 at 155.861 kb on - strand, within Pf6N2E2_110 at 155.905 kb on + strand, within Pf6N2E2_110 at 155.906 kb on - strand, within Pf6N2E2_110 at 155.906 kb on - strand, within Pf6N2E2_110 at 156.009 kb on + strand, within Pf6N2E2_110 at 156.009 kb on + strand, within Pf6N2E2_110 at 156.010 kb on - strand, within Pf6N2E2_110 at 156.274 kb on + strand, within Pf6N2E2_110 at 156.374 kb on + strand, within Pf6N2E2_110 at 156.658 kb on + strand, within Pf6N2E2_111 at 156.659 kb on - strand, within Pf6N2E2_111 at 156.800 kb on + strand, within Pf6N2E2_111 at 156.800 kb on + strand, within Pf6N2E2_111 at 156.800 kb on + strand, within Pf6N2E2_111 at 156.800 kb on + strand, within Pf6N2E2_111 at 156.800 kb on + strand, within Pf6N2E2_111 at 156.806 kb on + strand, within Pf6N2E2_111 at 156.806 kb on + strand, within Pf6N2E2_111 at 156.806 kb on + strand, within Pf6N2E2_111 at 156.806 kb on + strand, within Pf6N2E2_111 at 156.807 kb on - strand, within Pf6N2E2_111 at 156.807 kb on - strand, within Pf6N2E2_111 at 156.827 kb on - strand, within Pf6N2E2_111 at 157.148 kb on - strand, within Pf6N2E2_111
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 154,731 + Pf6N2E2_109 0.13 +0.8 154,732 - Pf6N2E2_109 0.13 +0.3 154,920 + Pf6N2E2_109 0.35 +0.7 154,920 + Pf6N2E2_109 0.35 -0.8 154,920 + Pf6N2E2_109 0.35 +1.2 154,921 - Pf6N2E2_109 0.35 +0.1 154,921 - Pf6N2E2_109 0.35 +0.2 154,921 - Pf6N2E2_109 0.35 +0.0 154,921 - Pf6N2E2_109 0.35 +0.6 154,921 - Pf6N2E2_109 0.35 +0.3 154,921 - Pf6N2E2_109 0.35 +0.6 155,069 + Pf6N2E2_109 0.52 -0.9 155,070 - Pf6N2E2_109 0.52 -0.5 155,070 - Pf6N2E2_109 0.52 -0.4 155,070 - Pf6N2E2_109 0.52 +0.5 155,102 - Pf6N2E2_109 0.56 +0.9 155,138 + Pf6N2E2_109 0.60 +2.4 155,138 + Pf6N2E2_109 0.60 -0.5 155,138 + Pf6N2E2_109 0.60 +0.8 155,235 + Pf6N2E2_109 0.71 -0.2 155,279 + Pf6N2E2_109 0.76 +0.7 155,280 - Pf6N2E2_109 0.76 +0.1 155,448 + +0.4 155,448 + +0.3 155,448 + +0.7 155,448 + -0.1 155,448 + -0.3 155,448 + +1.3 155,449 - -0.1 155,449 - +1.5 155,449 - +0.0 155,449 - +0.0 155,545 + -0.2 155,546 - +0.3 155,546 - -0.2 155,591 - -0.1 155,640 + +0.0 155,662 + +1.3 155,769 + Pf6N2E2_110 0.11 +0.3 155,769 + Pf6N2E2_110 0.11 -1.6 155,770 - Pf6N2E2_110 0.12 -0.2 155,860 + Pf6N2E2_110 0.23 -0.8 155,860 + Pf6N2E2_110 0.23 -0.6 155,861 - Pf6N2E2_110 0.23 -0.6 155,861 - Pf6N2E2_110 0.23 +0.3 155,861 - Pf6N2E2_110 0.23 +1.3 155,905 + Pf6N2E2_110 0.29 +0.7 155,906 - Pf6N2E2_110 0.29 +2.8 155,906 - Pf6N2E2_110 0.29 -1.6 156,009 + Pf6N2E2_110 0.42 +0.9 156,009 + Pf6N2E2_110 0.42 -2.2 156,010 - Pf6N2E2_110 0.42 -0.1 156,274 + Pf6N2E2_110 0.76 -0.5 156,374 + Pf6N2E2_110 0.89 -0.0 156,658 + Pf6N2E2_111 0.18 +0.0 156,659 - Pf6N2E2_111 0.18 +0.2 156,800 + Pf6N2E2_111 0.30 +0.1 156,800 + Pf6N2E2_111 0.30 +0.7 156,800 + Pf6N2E2_111 0.30 -0.1 156,800 + Pf6N2E2_111 0.30 +0.1 156,800 + Pf6N2E2_111 0.30 +0.3 156,806 + Pf6N2E2_111 0.31 +0.5 156,806 + Pf6N2E2_111 0.31 -0.6 156,806 + Pf6N2E2_111 0.31 +0.0 156,806 + Pf6N2E2_111 0.31 -1.8 156,807 - Pf6N2E2_111 0.31 -0.2 156,807 - Pf6N2E2_111 0.31 +0.6 156,827 - Pf6N2E2_111 0.32 -0.7 157,148 - Pf6N2E2_111 0.60 +0.4
Or see this region's nucleotide sequence