Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1070 and Pf6N2E2_1071 are separated by 13 nucleotides Pf6N2E2_1071 and Pf6N2E2_1072 are separated by 23 nucleotides Pf6N2E2_1072 and Pf6N2E2_1073 are separated by 250 nucleotides
Pf6N2E2_1070 - Endonuclease/Exonuclease/phosphatase family protein, at 1,249,893 to 1,250,693
_1070
Pf6N2E2_1071 - hypothetical protein, at 1,250,707 to 1,250,838
_1071
Pf6N2E2_1072 - LmbE-like protein, at 1,250,862 to 1,251,626
_1072
Pf6N2E2_1073 - Glycogen debranching enzyme (EC 3.2.1.-), at 1,251,877 to 1,254,036
_1073
Position (kb)
1250
1251
1252 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1249.887 kb on + strand at 1249.887 kb on + strand at 1249.887 kb on + strand at 1249.894 kb on + strand at 1249.993 kb on + strand, within Pf6N2E2_1070 at 1249.994 kb on - strand, within Pf6N2E2_1070 at 1249.994 kb on - strand, within Pf6N2E2_1070 at 1249.994 kb on - strand, within Pf6N2E2_1070 at 1249.997 kb on + strand, within Pf6N2E2_1070 at 1249.997 kb on + strand, within Pf6N2E2_1070 at 1249.997 kb on + strand, within Pf6N2E2_1070 at 1249.997 kb on + strand, within Pf6N2E2_1070 at 1249.997 kb on + strand, within Pf6N2E2_1070 at 1249.998 kb on - strand, within Pf6N2E2_1070 at 1249.998 kb on - strand, within Pf6N2E2_1070 at 1249.998 kb on - strand, within Pf6N2E2_1070 at 1249.998 kb on - strand, within Pf6N2E2_1070 at 1250.032 kb on + strand, within Pf6N2E2_1070 at 1250.033 kb on - strand, within Pf6N2E2_1070 at 1250.080 kb on + strand, within Pf6N2E2_1070 at 1250.081 kb on - strand, within Pf6N2E2_1070 at 1250.714 kb on + strand at 1250.714 kb on + strand at 1250.714 kb on + strand at 1250.715 kb on - strand at 1250.715 kb on - strand at 1250.715 kb on - strand at 1250.715 kb on - strand at 1250.715 kb on - strand at 1250.715 kb on - strand at 1250.849 kb on + strand at 1250.965 kb on + strand, within Pf6N2E2_1072 at 1250.965 kb on + strand, within Pf6N2E2_1072 at 1250.965 kb on + strand, within Pf6N2E2_1072 at 1250.966 kb on - strand, within Pf6N2E2_1072 at 1250.966 kb on - strand, within Pf6N2E2_1072 at 1250.966 kb on - strand, within Pf6N2E2_1072 at 1250.971 kb on + strand, within Pf6N2E2_1072 at 1250.971 kb on + strand, within Pf6N2E2_1072 at 1250.971 kb on + strand, within Pf6N2E2_1072 at 1250.972 kb on - strand, within Pf6N2E2_1072 at 1250.972 kb on - strand, within Pf6N2E2_1072 at 1250.972 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.351 kb on - strand, within Pf6N2E2_1072 at 1251.646 kb on - strand at 1251.674 kb on + strand at 1251.750 kb on + strand at 1251.750 kb on + strand at 1251.750 kb on + strand at 1251.750 kb on + strand at 1251.751 kb on - strand at 1251.751 kb on - strand at 1251.751 kb on - strand at 1251.864 kb on + strand at 1251.874 kb on - strand at 1251.936 kb on - strand at 1252.072 kb on + strand at 1252.073 kb on - strand at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.238 kb on + strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.239 kb on - strand, within Pf6N2E2_1073 at 1252.379 kb on + strand, within Pf6N2E2_1073 at 1252.379 kb on + strand, within Pf6N2E2_1073 at 1252.380 kb on - strand, within Pf6N2E2_1073 at 1252.380 kb on - strand, within Pf6N2E2_1073 at 1252.389 kb on + strand, within Pf6N2E2_1073 at 1252.441 kb on - strand, within Pf6N2E2_1073 at 1252.607 kb on + strand, within Pf6N2E2_1073 at 1252.607 kb on + strand, within Pf6N2E2_1073 at 1252.608 kb on - strand, within Pf6N2E2_1073 at 1252.608 kb on - strand, within Pf6N2E2_1073
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,249,887 + +0.2 1,249,887 + -0.6 1,249,887 + +0.4 1,249,894 + -0.0 1,249,993 + Pf6N2E2_1070 0.12 +0.5 1,249,994 - Pf6N2E2_1070 0.13 -0.3 1,249,994 - Pf6N2E2_1070 0.13 +0.2 1,249,994 - Pf6N2E2_1070 0.13 -0.2 1,249,997 + Pf6N2E2_1070 0.13 +0.8 1,249,997 + Pf6N2E2_1070 0.13 +0.8 1,249,997 + Pf6N2E2_1070 0.13 -1.2 1,249,997 + Pf6N2E2_1070 0.13 +0.4 1,249,997 + Pf6N2E2_1070 0.13 +1.0 1,249,998 - Pf6N2E2_1070 0.13 -0.5 1,249,998 - Pf6N2E2_1070 0.13 -0.4 1,249,998 - Pf6N2E2_1070 0.13 +1.1 1,249,998 - Pf6N2E2_1070 0.13 -0.6 1,250,032 + Pf6N2E2_1070 0.17 -0.1 1,250,033 - Pf6N2E2_1070 0.17 -1.1 1,250,080 + Pf6N2E2_1070 0.23 +0.8 1,250,081 - Pf6N2E2_1070 0.23 -1.8 1,250,714 + -2.4 1,250,714 + +0.7 1,250,714 + -1.1 1,250,715 - +1.8 1,250,715 - -1.3 1,250,715 - +0.2 1,250,715 - +0.2 1,250,715 - +0.3 1,250,715 - +0.4 1,250,849 + -0.8 1,250,965 + Pf6N2E2_1072 0.13 -0.5 1,250,965 + Pf6N2E2_1072 0.13 -1.1 1,250,965 + Pf6N2E2_1072 0.13 -0.6 1,250,966 - Pf6N2E2_1072 0.14 +0.9 1,250,966 - Pf6N2E2_1072 0.14 -1.8 1,250,966 - Pf6N2E2_1072 0.14 -1.2 1,250,971 + Pf6N2E2_1072 0.14 -1.8 1,250,971 + Pf6N2E2_1072 0.14 -1.1 1,250,971 + Pf6N2E2_1072 0.14 -0.7 1,250,972 - Pf6N2E2_1072 0.14 -0.3 1,250,972 - Pf6N2E2_1072 0.14 -0.8 1,250,972 - Pf6N2E2_1072 0.14 -0.9 1,251,351 - Pf6N2E2_1072 0.64 -1.1 1,251,351 - Pf6N2E2_1072 0.64 -0.2 1,251,351 - Pf6N2E2_1072 0.64 -0.5 1,251,351 - Pf6N2E2_1072 0.64 -1.1 1,251,646 - +1.7 1,251,674 + -0.6 1,251,750 + -0.5 1,251,750 + -0.7 1,251,750 + -0.3 1,251,750 + -1.1 1,251,751 - +0.7 1,251,751 - +1.3 1,251,751 - -0.3 1,251,864 + +1.2 1,251,874 - +0.6 1,251,936 - +0.0 1,252,072 + -0.3 1,252,073 - -1.0 1,252,238 + Pf6N2E2_1073 0.17 +0.5 1,252,238 + Pf6N2E2_1073 0.17 +0.9 1,252,238 + Pf6N2E2_1073 0.17 +0.6 1,252,238 + Pf6N2E2_1073 0.17 +0.0 1,252,238 + Pf6N2E2_1073 0.17 -0.6 1,252,239 - Pf6N2E2_1073 0.17 -0.5 1,252,239 - Pf6N2E2_1073 0.17 +1.3 1,252,239 - Pf6N2E2_1073 0.17 -0.3 1,252,239 - Pf6N2E2_1073 0.17 +0.4 1,252,239 - Pf6N2E2_1073 0.17 -0.3 1,252,239 - Pf6N2E2_1073 0.17 +0.5 1,252,239 - Pf6N2E2_1073 0.17 +0.8 1,252,239 - Pf6N2E2_1073 0.17 -1.4 1,252,239 - Pf6N2E2_1073 0.17 -1.0 1,252,239 - Pf6N2E2_1073 0.17 +1.1 1,252,239 - Pf6N2E2_1073 0.17 +0.4 1,252,239 - Pf6N2E2_1073 0.17 +0.3 1,252,239 - Pf6N2E2_1073 0.17 -0.6 1,252,379 + Pf6N2E2_1073 0.23 +0.6 1,252,379 + Pf6N2E2_1073 0.23 +1.1 1,252,380 - Pf6N2E2_1073 0.23 +2.3 1,252,380 - Pf6N2E2_1073 0.23 +2.1 1,252,389 + Pf6N2E2_1073 0.24 +0.1 1,252,441 - Pf6N2E2_1073 0.26 +0.6 1,252,607 + Pf6N2E2_1073 0.34 -0.8 1,252,607 + Pf6N2E2_1073 0.34 +1.0 1,252,608 - Pf6N2E2_1073 0.34 -0.3 1,252,608 - Pf6N2E2_1073 0.34 +0.3
Or see this region's nucleotide sequence