Experiment: PYE with CHIR-090 2 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01448 and CCNA_01449 are separated by 60 nucleotides CCNA_01449 and CCNA_01450 are separated by 67 nucleotides
CCNA_01448: CCNA_01448 - fructose-1,6-bisphosphatase, class II, at 1,560,867 to 1,561,820
_01448
CCNA_01449: CCNA_01449 - fructose-1,6-bisphosphatase, FIG superfamily, at 1,561,881 to 1,562,858
_01449
CCNA_01450: CCNA_01450 - single-stranded-DNA-specific exonuclease recJ, at 1,562,926 to 1,564,746
_01450
Position (kb)
1561
1562
1563 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1560.956 kb on - strand at 1561.087 kb on - strand, within CCNA_01448 at 1561.180 kb on - strand, within CCNA_01448 at 1561.202 kb on + strand, within CCNA_01448 at 1561.215 kb on + strand, within CCNA_01448 at 1561.242 kb on - strand, within CCNA_01448 at 1561.328 kb on + strand, within CCNA_01448 at 1561.336 kb on - strand, within CCNA_01448 at 1561.336 kb on - strand, within CCNA_01448 at 1561.353 kb on + strand, within CCNA_01448 at 1561.413 kb on + strand, within CCNA_01448 at 1561.468 kb on - strand, within CCNA_01448 at 1561.481 kb on - strand, within CCNA_01448 at 1561.486 kb on + strand, within CCNA_01448 at 1561.493 kb on + strand, within CCNA_01448 at 1561.527 kb on + strand, within CCNA_01448 at 1561.527 kb on + strand, within CCNA_01448 at 1561.527 kb on + strand, within CCNA_01448 at 1561.535 kb on - strand, within CCNA_01448 at 1561.540 kb on - strand, within CCNA_01448 at 1561.663 kb on - strand, within CCNA_01448 at 1561.751 kb on + strand at 1561.753 kb on + strand at 1561.761 kb on - strand at 1561.761 kb on - strand at 1561.788 kb on + strand at 1561.796 kb on + strand at 1561.823 kb on - strand at 1561.823 kb on - strand at 1561.849 kb on - strand at 1561.949 kb on + strand at 1561.950 kb on + strand at 1561.950 kb on + strand at 1561.950 kb on + strand at 1561.994 kb on + strand, within CCNA_01449 at 1561.999 kb on + strand, within CCNA_01449 at 1562.030 kb on + strand, within CCNA_01449 at 1562.101 kb on + strand, within CCNA_01449 at 1562.153 kb on + strand, within CCNA_01449 at 1562.153 kb on + strand, within CCNA_01449 at 1562.330 kb on + strand, within CCNA_01449 at 1562.439 kb on - strand, within CCNA_01449 at 1562.693 kb on + strand, within CCNA_01449 at 1562.779 kb on + strand at 1562.863 kb on + strand at 1562.910 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 2 ug/mL remove 1,560,956 - -0.6 1,561,087 - CCNA_01448 0.23 -1.2 1,561,180 - CCNA_01448 0.33 +0.1 1,561,202 + CCNA_01448 0.35 -1.3 1,561,215 + CCNA_01448 0.36 -0.1 1,561,242 - CCNA_01448 0.39 +0.8 1,561,328 + CCNA_01448 0.48 -0.0 1,561,336 - CCNA_01448 0.49 -1.3 1,561,336 - CCNA_01448 0.49 -1.1 1,561,353 + CCNA_01448 0.51 +0.6 1,561,413 + CCNA_01448 0.57 -0.1 1,561,468 - CCNA_01448 0.63 -0.9 1,561,481 - CCNA_01448 0.64 +2.7 1,561,486 + CCNA_01448 0.65 +0.4 1,561,493 + CCNA_01448 0.66 -0.2 1,561,527 + CCNA_01448 0.69 +1.0 1,561,527 + CCNA_01448 0.69 +0.7 1,561,527 + CCNA_01448 0.69 +0.4 1,561,535 - CCNA_01448 0.70 -1.9 1,561,540 - CCNA_01448 0.71 +1.5 1,561,663 - CCNA_01448 0.83 -1.3 1,561,751 + -0.7 1,561,753 + -1.2 1,561,761 - -1.2 1,561,761 - -0.8 1,561,788 + +0.1 1,561,796 + +0.5 1,561,823 - -2.2 1,561,823 - -0.1 1,561,849 - +2.7 1,561,949 + +1.4 1,561,950 + -1.0 1,561,950 + -1.6 1,561,950 + +0.9 1,561,994 + CCNA_01449 0.12 -0.2 1,561,999 + CCNA_01449 0.12 +0.1 1,562,030 + CCNA_01449 0.15 +2.1 1,562,101 + CCNA_01449 0.22 -1.8 1,562,153 + CCNA_01449 0.28 -0.6 1,562,153 + CCNA_01449 0.28 -0.8 1,562,330 + CCNA_01449 0.46 +0.1 1,562,439 - CCNA_01449 0.57 -0.3 1,562,693 + CCNA_01449 0.83 +2.1 1,562,779 + +0.9 1,562,863 + -0.2 1,562,910 + +0.8
Or see this region's nucleotide sequence