Experiment: PYE with CHIR-090 2 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01395 and CCNA_01396 are separated by 74 nucleotides CCNA_01396 and CCNA_01397 are separated by 41 nucleotides CCNA_01397 and CCNA_01398 are separated by 7 nucleotides
CCNA_01395: CCNA_01395 - electron transfer flavoprotein-ubiquinone oxidoreductase FzeA, at 1,513,437 to 1,515,113
_01395
CCNA_01396: CCNA_01396 - tetratricopeptide repeat family protein, at 1,515,188 to 1,516,918
_01396
CCNA_01397: CCNA_01397 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 1,516,960 to 1,517,832
_01397
CCNA_01398: CCNA_01398 - peptidyl-prolyl cis-trans isomerase, at 1,517,840 to 1,518,478
_01398
Position (kb)
1515
1516
1517 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1515.163 kb on + strand at 1515.163 kb on + strand at 1515.171 kb on - strand at 1515.204 kb on - strand at 1515.233 kb on + strand at 1515.289 kb on + strand at 1515.297 kb on - strand at 1515.385 kb on + strand, within CCNA_01396 at 1515.408 kb on + strand, within CCNA_01396 at 1515.444 kb on + strand, within CCNA_01396 at 1515.459 kb on - strand, within CCNA_01396 at 1515.461 kb on + strand, within CCNA_01396 at 1515.502 kb on + strand, within CCNA_01396 at 1515.508 kb on + strand, within CCNA_01396 at 1515.508 kb on + strand, within CCNA_01396 at 1515.515 kb on - strand, within CCNA_01396 at 1515.516 kb on - strand, within CCNA_01396 at 1515.516 kb on - strand, within CCNA_01396 at 1515.533 kb on + strand, within CCNA_01396 at 1515.533 kb on + strand, within CCNA_01396 at 1515.533 kb on + strand, within CCNA_01396 at 1515.541 kb on - strand, within CCNA_01396 at 1515.541 kb on - strand, within CCNA_01396 at 1515.562 kb on - strand, within CCNA_01396 at 1515.571 kb on + strand, within CCNA_01396 at 1515.571 kb on + strand, within CCNA_01396 at 1515.571 kb on + strand, within CCNA_01396 at 1515.571 kb on + strand, within CCNA_01396 at 1515.571 kb on + strand, within CCNA_01396 at 1515.579 kb on - strand, within CCNA_01396 at 1515.579 kb on - strand, within CCNA_01396 at 1515.579 kb on - strand, within CCNA_01396 at 1515.579 kb on - strand, within CCNA_01396 at 1515.590 kb on + strand, within CCNA_01396 at 1515.592 kb on + strand, within CCNA_01396 at 1515.597 kb on + strand, within CCNA_01396 at 1515.600 kb on - strand, within CCNA_01396 at 1515.600 kb on - strand, within CCNA_01396 at 1515.605 kb on - strand, within CCNA_01396 at 1515.638 kb on - strand, within CCNA_01396 at 1515.640 kb on + strand, within CCNA_01396 at 1515.643 kb on - strand, within CCNA_01396 at 1515.654 kb on - strand, within CCNA_01396 at 1515.688 kb on + strand, within CCNA_01396 at 1515.709 kb on + strand, within CCNA_01396 at 1515.724 kb on + strand, within CCNA_01396 at 1515.781 kb on + strand, within CCNA_01396 at 1515.789 kb on - strand, within CCNA_01396 at 1515.790 kb on - strand, within CCNA_01396 at 1515.800 kb on + strand, within CCNA_01396 at 1515.805 kb on + strand, within CCNA_01396 at 1515.807 kb on + strand, within CCNA_01396 at 1515.808 kb on - strand, within CCNA_01396 at 1515.871 kb on + strand, within CCNA_01396 at 1515.879 kb on - strand, within CCNA_01396 at 1515.895 kb on + strand, within CCNA_01396 at 1515.903 kb on - strand, within CCNA_01396 at 1515.903 kb on - strand, within CCNA_01396 at 1515.903 kb on - strand, within CCNA_01396 at 1515.908 kb on - strand, within CCNA_01396 at 1515.932 kb on + strand, within CCNA_01396 at 1515.935 kb on - strand, within CCNA_01396 at 1515.935 kb on - strand, within CCNA_01396 at 1515.935 kb on - strand, within CCNA_01396 at 1515.940 kb on - strand, within CCNA_01396 at 1515.990 kb on + strand, within CCNA_01396 at 1516.020 kb on - strand, within CCNA_01396 at 1516.074 kb on + strand, within CCNA_01396 at 1516.121 kb on + strand, within CCNA_01396 at 1516.136 kb on + strand, within CCNA_01396 at 1516.152 kb on + strand, within CCNA_01396 at 1516.205 kb on + strand, within CCNA_01396 at 1516.205 kb on + strand, within CCNA_01396 at 1516.212 kb on - strand, within CCNA_01396 at 1516.217 kb on + strand, within CCNA_01396 at 1516.222 kb on + strand, within CCNA_01396 at 1516.222 kb on + strand, within CCNA_01396 at 1516.222 kb on + strand, within CCNA_01396 at 1516.230 kb on - strand, within CCNA_01396 at 1516.230 kb on - strand, within CCNA_01396 at 1516.245 kb on - strand, within CCNA_01396 at 1516.260 kb on + strand, within CCNA_01396 at 1516.274 kb on + strand, within CCNA_01396 at 1516.274 kb on + strand, within CCNA_01396 at 1516.289 kb on + strand, within CCNA_01396 at 1516.291 kb on + strand, within CCNA_01396 at 1516.291 kb on + strand, within CCNA_01396 at 1516.299 kb on - strand, within CCNA_01396 at 1516.322 kb on + strand, within CCNA_01396 at 1516.340 kb on - strand, within CCNA_01396 at 1516.376 kb on - strand, within CCNA_01396 at 1516.379 kb on + strand, within CCNA_01396 at 1516.406 kb on + strand, within CCNA_01396 at 1516.437 kb on - strand, within CCNA_01396 at 1516.438 kb on - strand, within CCNA_01396 at 1516.438 kb on - strand, within CCNA_01396 at 1516.438 kb on - strand, within CCNA_01396 at 1516.449 kb on + strand, within CCNA_01396 at 1516.449 kb on + strand, within CCNA_01396 at 1516.453 kb on + strand, within CCNA_01396 at 1516.457 kb on - strand, within CCNA_01396 at 1516.529 kb on + strand, within CCNA_01396 at 1516.560 kb on + strand, within CCNA_01396 at 1516.576 kb on + strand, within CCNA_01396 at 1516.583 kb on + strand, within CCNA_01396 at 1516.584 kb on - strand, within CCNA_01396 at 1516.655 kb on + strand, within CCNA_01396 at 1516.675 kb on + strand, within CCNA_01396 at 1516.677 kb on + strand, within CCNA_01396 at 1516.683 kb on - strand, within CCNA_01396 at 1516.713 kb on + strand, within CCNA_01396 at 1516.770 kb on - strand at 1516.781 kb on + strand at 1516.811 kb on - strand at 1516.815 kb on + strand at 1516.823 kb on - strand at 1516.823 kb on - strand at 1516.823 kb on - strand at 1516.823 kb on - strand at 1516.823 kb on - strand at 1516.823 kb on - strand at 1516.834 kb on + strand at 1516.846 kb on + strand at 1516.869 kb on + strand at 1516.869 kb on + strand at 1516.869 kb on + strand at 1516.869 kb on + strand at 1516.876 kb on - strand at 1516.912 kb on + strand at 1516.915 kb on - strand at 1516.934 kb on - strand at 1516.936 kb on + strand at 1517.902 kb on - strand at 1517.902 kb on - strand at 1517.913 kb on + strand, within CCNA_01398
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 2 ug/mL remove 1,515,163 + +0.5 1,515,163 + -1.3 1,515,171 - +0.8 1,515,204 - +0.3 1,515,233 + -0.8 1,515,289 + +1.0 1,515,297 - +0.1 1,515,385 + CCNA_01396 0.11 -0.9 1,515,408 + CCNA_01396 0.13 -1.7 1,515,444 + CCNA_01396 0.15 -1.8 1,515,459 - CCNA_01396 0.16 -2.2 1,515,461 + CCNA_01396 0.16 -1.1 1,515,502 + CCNA_01396 0.18 -1.2 1,515,508 + CCNA_01396 0.18 +1.4 1,515,508 + CCNA_01396 0.18 +0.7 1,515,515 - CCNA_01396 0.19 +0.4 1,515,516 - CCNA_01396 0.19 -1.1 1,515,516 - CCNA_01396 0.19 -1.0 1,515,533 + CCNA_01396 0.20 -0.9 1,515,533 + CCNA_01396 0.20 -1.8 1,515,533 + CCNA_01396 0.20 -1.7 1,515,541 - CCNA_01396 0.20 +1.0 1,515,541 - CCNA_01396 0.20 -1.3 1,515,562 - CCNA_01396 0.22 +0.2 1,515,571 + CCNA_01396 0.22 -0.6 1,515,571 + CCNA_01396 0.22 -1.2 1,515,571 + CCNA_01396 0.22 -1.8 1,515,571 + CCNA_01396 0.22 -0.6 1,515,571 + CCNA_01396 0.22 -0.2 1,515,579 - CCNA_01396 0.23 -0.1 1,515,579 - CCNA_01396 0.23 -0.5 1,515,579 - CCNA_01396 0.23 -1.4 1,515,579 - CCNA_01396 0.23 -0.7 1,515,590 + CCNA_01396 0.23 -1.1 1,515,592 + CCNA_01396 0.23 -0.8 1,515,597 + CCNA_01396 0.24 +0.2 1,515,600 - CCNA_01396 0.24 +0.7 1,515,600 - CCNA_01396 0.24 +0.0 1,515,605 - CCNA_01396 0.24 -0.8 1,515,638 - CCNA_01396 0.26 -1.7 1,515,640 + CCNA_01396 0.26 +0.5 1,515,643 - CCNA_01396 0.26 -0.7 1,515,654 - CCNA_01396 0.27 -2.2 1,515,688 + CCNA_01396 0.29 -1.4 1,515,709 + CCNA_01396 0.30 +0.0 1,515,724 + CCNA_01396 0.31 -1.3 1,515,781 + CCNA_01396 0.34 +0.0 1,515,789 - CCNA_01396 0.35 -1.2 1,515,790 - CCNA_01396 0.35 -1.1 1,515,800 + CCNA_01396 0.35 +0.0 1,515,805 + CCNA_01396 0.36 +0.3 1,515,807 + CCNA_01396 0.36 -0.1 1,515,808 - CCNA_01396 0.36 -1.8 1,515,871 + CCNA_01396 0.39 -0.7 1,515,879 - CCNA_01396 0.40 -0.5 1,515,895 + CCNA_01396 0.41 -0.7 1,515,903 - CCNA_01396 0.41 -1.7 1,515,903 - CCNA_01396 0.41 +1.7 1,515,903 - CCNA_01396 0.41 +1.4 1,515,908 - CCNA_01396 0.42 -0.0 1,515,932 + CCNA_01396 0.43 -0.6 1,515,935 - CCNA_01396 0.43 -0.6 1,515,935 - CCNA_01396 0.43 -1.8 1,515,935 - CCNA_01396 0.43 -1.2 1,515,940 - CCNA_01396 0.43 -1.3 1,515,990 + CCNA_01396 0.46 +2.1 1,516,020 - CCNA_01396 0.48 -0.9 1,516,074 + CCNA_01396 0.51 -0.3 1,516,121 + CCNA_01396 0.54 -1.1 1,516,136 + CCNA_01396 0.55 -0.9 1,516,152 + CCNA_01396 0.56 -1.5 1,516,205 + CCNA_01396 0.59 -0.6 1,516,205 + CCNA_01396 0.59 -1.2 1,516,212 - CCNA_01396 0.59 -0.1 1,516,217 + CCNA_01396 0.59 -1.9 1,516,222 + CCNA_01396 0.60 -1.7 1,516,222 + CCNA_01396 0.60 -1.3 1,516,222 + CCNA_01396 0.60 -1.2 1,516,230 - CCNA_01396 0.60 +1.7 1,516,230 - CCNA_01396 0.60 -1.8 1,516,245 - CCNA_01396 0.61 -0.7 1,516,260 + CCNA_01396 0.62 -1.2 1,516,274 + CCNA_01396 0.63 -0.9 1,516,274 + CCNA_01396 0.63 -0.1 1,516,289 + CCNA_01396 0.64 +0.5 1,516,291 + CCNA_01396 0.64 -0.9 1,516,291 + CCNA_01396 0.64 -2.5 1,516,299 - CCNA_01396 0.64 +2.0 1,516,322 + CCNA_01396 0.66 -1.0 1,516,340 - CCNA_01396 0.67 -1.4 1,516,376 - CCNA_01396 0.69 -0.9 1,516,379 + CCNA_01396 0.69 -0.9 1,516,406 + CCNA_01396 0.70 -0.6 1,516,437 - CCNA_01396 0.72 +0.4 1,516,438 - CCNA_01396 0.72 -2.1 1,516,438 - CCNA_01396 0.72 -0.6 1,516,438 - CCNA_01396 0.72 -0.2 1,516,449 + CCNA_01396 0.73 -0.5 1,516,449 + CCNA_01396 0.73 -2.6 1,516,453 + CCNA_01396 0.73 +0.8 1,516,457 - CCNA_01396 0.73 -0.9 1,516,529 + CCNA_01396 0.77 -1.8 1,516,560 + CCNA_01396 0.79 -1.5 1,516,576 + CCNA_01396 0.80 -3.0 1,516,583 + CCNA_01396 0.81 +0.6 1,516,584 - CCNA_01396 0.81 -0.3 1,516,655 + CCNA_01396 0.85 -0.3 1,516,675 + CCNA_01396 0.86 -1.5 1,516,677 + CCNA_01396 0.86 -1.0 1,516,683 - CCNA_01396 0.86 -2.3 1,516,713 + CCNA_01396 0.88 -1.2 1,516,770 - -0.3 1,516,781 + -1.8 1,516,811 - +0.1 1,516,815 + -0.7 1,516,823 - +1.1 1,516,823 - +0.4 1,516,823 - +1.7 1,516,823 - -2.4 1,516,823 - +0.0 1,516,823 - +0.0 1,516,834 + -0.1 1,516,846 + -1.2 1,516,869 + -0.7 1,516,869 + -1.1 1,516,869 + -0.6 1,516,869 + -0.9 1,516,876 - -1.2 1,516,912 + -0.7 1,516,915 - -1.1 1,516,934 - +0.1 1,516,936 + -0.3 1,517,902 - +0.0 1,517,902 - -0.8 1,517,913 + CCNA_01398 0.11 -0.4
Or see this region's nucleotide sequence