Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27210 and BWI76_RS27215 overlap by 1 nucleotides BWI76_RS27215 and BWI76_RS27220 are separated by 59 nucleotides BWI76_RS27220 and BWI76_RS27225 are separated by 111 nucleotides BWI76_RS27225 and BWI76_RS27230 are separated by 245 nucleotides
BWI76_RS27210: BWI76_RS27210 - glycerol-3-phosphate dehydrogenase, at 5,648,179 to 5,649,195
_RS27210
BWI76_RS27215: BWI76_RS27215 - protein-export protein SecB, at 5,649,195 to 5,649,662
_RS27215
BWI76_RS27220: BWI76_RS27220 - glutaredoxin 3, at 5,649,722 to 5,649,973
_RS27220
BWI76_RS27225: BWI76_RS27225 - rhodanese-like domain-containing protein, at 5,650,085 to 5,650,516
_RS27225
BWI76_RS27230: BWI76_RS27230 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 5,650,762 to 5,652,306
_RS27230
Position (kb) 5649
5650 Strain fitness (log2 ratio) -1
0
1 at 5649.738 kb on - strand at 5649.898 kb on - strand, within BWI76_RS27220 at 5649.958 kb on - strand at 5650.117 kb on - strand at 5650.164 kb on - strand, within BWI76_RS27225 at 5650.175 kb on - strand, within BWI76_RS27225 at 5650.472 kb on + strand, within BWI76_RS27225 at 5650.561 kb on + strand at 5650.569 kb on - strand at 5650.686 kb on - strand at 5650.861 kb on + strand at 5650.957 kb on - strand, within BWI76_RS27230 at 5650.957 kb on - strand, within BWI76_RS27230 at 5650.965 kb on + strand, within BWI76_RS27230
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 5,649,738 - +0.3 5,649,898 - BWI76_RS27220 0.70 +0.5 5,649,958 - +0.7 5,650,117 - -0.4 5,650,164 - BWI76_RS27225 0.18 +0.3 5,650,175 - BWI76_RS27225 0.21 -1.2 5,650,472 + BWI76_RS27225 0.90 +0.9 5,650,561 + +0.3 5,650,569 - +0.3 5,650,686 - +1.0 5,650,861 + -0.3 5,650,957 - BWI76_RS27230 0.13 -1.3 5,650,957 - BWI76_RS27230 0.13 -0.3 5,650,965 + BWI76_RS27230 0.13 -1.4
Or see this region's nucleotide sequence