Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27195 and BWI76_RS27200 are separated by 49 nucleotides BWI76_RS27200 and BWI76_RS27205 are separated by 105 nucleotides
BWI76_RS27195: BWI76_RS27195 - alpha-hydroxy-acid oxidizing enzyme, at 5,645,418 to 5,646,648
_RS27195
BWI76_RS27200: BWI76_RS27200 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 5,646,698 to 5,647,171
_RS27200
BWI76_RS27205: BWI76_RS27205 - serine O-acetyltransferase, at 5,647,277 to 5,648,098
_RS27205
Position (kb) 5646
5647
5648 Strain fitness (log2 ratio) -1
0
1 at 5645.871 kb on - strand, within BWI76_RS27195 at 5645.871 kb on - strand, within BWI76_RS27195 at 5645.873 kb on + strand, within BWI76_RS27195 at 5645.873 kb on + strand, within BWI76_RS27195 at 5646.072 kb on + strand, within BWI76_RS27195 at 5646.266 kb on + strand, within BWI76_RS27195 at 5646.406 kb on + strand, within BWI76_RS27195 at 5646.651 kb on - strand at 5646.652 kb on - strand at 5646.866 kb on + strand, within BWI76_RS27200 at 5646.924 kb on - strand, within BWI76_RS27200 at 5646.966 kb on + strand, within BWI76_RS27200 at 5647.005 kb on + strand, within BWI76_RS27200 at 5647.322 kb on + strand at 5648.103 kb on - strand at 5648.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 5,645,871 - BWI76_RS27195 0.37 -0.3 5,645,871 - BWI76_RS27195 0.37 +1.4 5,645,873 + BWI76_RS27195 0.37 -0.8 5,645,873 + BWI76_RS27195 0.37 +0.2 5,646,072 + BWI76_RS27195 0.53 +0.1 5,646,266 + BWI76_RS27195 0.69 -1.4 5,646,406 + BWI76_RS27195 0.80 +0.0 5,646,651 - -0.3 5,646,652 - +0.2 5,646,866 + BWI76_RS27200 0.35 -0.4 5,646,924 - BWI76_RS27200 0.48 -1.0 5,646,966 + BWI76_RS27200 0.57 -0.1 5,647,005 + BWI76_RS27200 0.65 -0.7 5,647,322 + -1.7 5,648,103 - -0.6 5,648,103 - -0.7
Or see this region's nucleotide sequence