Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27165 and BWI76_RS27170 overlap by 4 nucleotides BWI76_RS27170 and BWI76_RS27175 overlap by 12 nucleotides BWI76_RS27175 and BWI76_RS27180 are separated by 274 nucleotides
BWI76_RS27165: BWI76_RS27165 - mannitol-1-phosphate 5-dehydrogenase, at 5,640,050 to 5,641,198
_RS27165
BWI76_RS27170: BWI76_RS27170 - MltR family transcriptional regulator, at 5,641,195 to 5,641,791
_RS27170
BWI76_RS27175: BWI76_RS27175 - hypothetical protein, at 5,641,780 to 5,642,004
_RS27175
BWI76_RS27180: BWI76_RS27180 - hypothetical protein, at 5,642,279 to 5,642,641
_RS27180
Position (kb)
5641
5642 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5640.225 kb on - strand, within BWI76_RS27165 at 5640.335 kb on + strand, within BWI76_RS27165 at 5640.444 kb on - strand, within BWI76_RS27165 at 5640.523 kb on - strand, within BWI76_RS27165 at 5640.613 kb on + strand, within BWI76_RS27165 at 5640.621 kb on - strand, within BWI76_RS27165 at 5640.621 kb on - strand, within BWI76_RS27165 at 5640.621 kb on - strand, within BWI76_RS27165 at 5640.686 kb on + strand, within BWI76_RS27165 at 5640.990 kb on - strand, within BWI76_RS27165 at 5640.996 kb on + strand, within BWI76_RS27165 at 5641.215 kb on + strand at 5641.434 kb on + strand, within BWI76_RS27170 at 5641.487 kb on + strand, within BWI76_RS27170 at 5641.495 kb on - strand, within BWI76_RS27170 at 5641.538 kb on - strand, within BWI76_RS27170 at 5641.549 kb on + strand, within BWI76_RS27170 at 5641.567 kb on + strand, within BWI76_RS27170 at 5641.570 kb on + strand, within BWI76_RS27170 at 5641.664 kb on + strand, within BWI76_RS27170 at 5641.705 kb on + strand, within BWI76_RS27170 at 5641.736 kb on - strand at 5641.736 kb on - strand at 5641.798 kb on - strand at 5641.839 kb on + strand, within BWI76_RS27175 at 5641.840 kb on - strand, within BWI76_RS27175 at 5641.889 kb on + strand, within BWI76_RS27175 at 5641.941 kb on - strand, within BWI76_RS27175 at 5642.008 kb on + strand at 5642.008 kb on + strand at 5642.011 kb on + strand at 5642.011 kb on + strand at 5642.013 kb on + strand at 5642.016 kb on - strand at 5642.016 kb on - strand at 5642.018 kb on + strand at 5642.019 kb on - strand at 5642.019 kb on - strand at 5642.019 kb on - strand at 5642.023 kb on + strand at 5642.031 kb on - strand at 5642.039 kb on + strand at 5642.085 kb on + strand at 5642.159 kb on - strand at 5642.218 kb on + strand at 5642.218 kb on + strand at 5642.226 kb on - strand at 5642.228 kb on - strand at 5642.230 kb on + strand at 5642.231 kb on - strand at 5642.232 kb on + strand at 5642.236 kb on + strand at 5642.238 kb on - strand at 5642.267 kb on + strand at 5642.323 kb on + strand, within BWI76_RS27180 at 5642.323 kb on + strand, within BWI76_RS27180 at 5642.323 kb on + strand, within BWI76_RS27180 at 5642.324 kb on - strand, within BWI76_RS27180 at 5642.348 kb on + strand, within BWI76_RS27180 at 5642.356 kb on - strand, within BWI76_RS27180 at 5642.362 kb on + strand, within BWI76_RS27180 at 5642.370 kb on - strand, within BWI76_RS27180 at 5642.440 kb on + strand, within BWI76_RS27180 at 5642.448 kb on + strand, within BWI76_RS27180 at 5642.449 kb on - strand, within BWI76_RS27180 at 5642.449 kb on - strand, within BWI76_RS27180 at 5642.458 kb on + strand, within BWI76_RS27180 at 5642.691 kb on - strand at 5642.767 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 5,640,225 - BWI76_RS27165 0.15 +1.9 5,640,335 + BWI76_RS27165 0.25 +0.3 5,640,444 - BWI76_RS27165 0.34 -1.1 5,640,523 - BWI76_RS27165 0.41 -0.2 5,640,613 + BWI76_RS27165 0.49 -0.9 5,640,621 - BWI76_RS27165 0.50 -1.1 5,640,621 - BWI76_RS27165 0.50 -1.4 5,640,621 - BWI76_RS27165 0.50 -0.6 5,640,686 + BWI76_RS27165 0.55 -0.1 5,640,990 - BWI76_RS27165 0.82 -1.4 5,640,996 + BWI76_RS27165 0.82 -0.4 5,641,215 + +0.9 5,641,434 + BWI76_RS27170 0.40 +0.1 5,641,487 + BWI76_RS27170 0.49 -0.9 5,641,495 - BWI76_RS27170 0.50 -0.4 5,641,538 - BWI76_RS27170 0.57 -1.8 5,641,549 + BWI76_RS27170 0.59 -0.7 5,641,567 + BWI76_RS27170 0.62 -2.3 5,641,570 + BWI76_RS27170 0.63 -0.5 5,641,664 + BWI76_RS27170 0.79 -0.6 5,641,705 + BWI76_RS27170 0.85 -0.6 5,641,736 - -0.6 5,641,736 - -0.7 5,641,798 - +0.3 5,641,839 + BWI76_RS27175 0.26 -0.6 5,641,840 - BWI76_RS27175 0.27 +1.1 5,641,889 + BWI76_RS27175 0.48 -0.4 5,641,941 - BWI76_RS27175 0.72 -0.1 5,642,008 + -0.6 5,642,008 + -0.2 5,642,011 + +2.2 5,642,011 + -0.5 5,642,013 + -0.2 5,642,016 - -0.2 5,642,016 - -0.0 5,642,018 + -1.2 5,642,019 - -0.2 5,642,019 - -0.9 5,642,019 - -1.0 5,642,023 + +0.3 5,642,031 - +0.5 5,642,039 + +0.3 5,642,085 + +0.1 5,642,159 - -0.2 5,642,218 + +0.5 5,642,218 + +0.3 5,642,226 - +0.3 5,642,228 - -0.8 5,642,230 + +1.8 5,642,231 - +0.8 5,642,232 + -0.3 5,642,236 + +0.5 5,642,238 - +0.2 5,642,267 + -1.6 5,642,323 + BWI76_RS27180 0.12 +0.1 5,642,323 + BWI76_RS27180 0.12 -0.8 5,642,323 + BWI76_RS27180 0.12 -0.4 5,642,324 - BWI76_RS27180 0.12 -0.0 5,642,348 + BWI76_RS27180 0.19 +0.3 5,642,356 - BWI76_RS27180 0.21 +0.3 5,642,362 + BWI76_RS27180 0.23 +0.7 5,642,370 - BWI76_RS27180 0.25 +0.2 5,642,440 + BWI76_RS27180 0.44 +0.8 5,642,448 + BWI76_RS27180 0.47 +0.1 5,642,449 - BWI76_RS27180 0.47 -0.6 5,642,449 - BWI76_RS27180 0.47 -0.1 5,642,458 + BWI76_RS27180 0.49 -0.5 5,642,691 - +1.6 5,642,767 + +0.9
Or see this region's nucleotide sequence