Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21085 and BWI76_RS21090 are separated by 26 nucleotides BWI76_RS21090 and BWI76_RS21095 are separated by 275 nucleotides
BWI76_RS21085: BWI76_RS21085 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin), at 4,411,249 to 4,412,370
_RS21085
BWI76_RS21090: BWI76_RS21090 - cytoskeletal protein RodZ, at 4,412,397 to 4,413,356
_RS21090
BWI76_RS21095: BWI76_RS21095 - bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN, at 4,413,632 to 4,414,798
_RS21095
Position (kb) 4412
4413
4414 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4412.571 kb on - strand, within BWI76_RS21090 at 4412.571 kb on - strand, within BWI76_RS21090 at 4412.757 kb on - strand, within BWI76_RS21090 at 4412.785 kb on - strand at 4413.410 kb on - strand at 4413.440 kb on + strand at 4413.450 kb on - strand at 4413.450 kb on - strand at 4413.612 kb on - strand at 4413.677 kb on - strand at 4413.677 kb on - strand at 4413.679 kb on + strand at 4414.089 kb on + strand, within BWI76_RS21095
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 4,412,571 - BWI76_RS21090 0.18 -0.5 4,412,571 - BWI76_RS21090 0.18 -2.5 4,412,757 - BWI76_RS21090 0.38 -0.3 4,412,785 - +1.4 4,413,410 - -0.9 4,413,440 + +0.5 4,413,450 - -0.0 4,413,450 - -0.3 4,413,612 - -3.4 4,413,677 - -0.2 4,413,677 - +0.2 4,413,679 + -0.2 4,414,089 + BWI76_RS21095 0.39 -0.0
Or see this region's nucleotide sequence