Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19575 and BWI76_RS19580 are separated by 8 nucleotides BWI76_RS19580 and BWI76_RS19585 are separated by 24 nucleotides BWI76_RS19585 and BWI76_RS19590 are separated by 11 nucleotides
BWI76_RS19575: BWI76_RS19575 - maleylacetoacetate isomerase, at 4,092,995 to 4,093,639
_RS19575
BWI76_RS19580: BWI76_RS19580 - FAA-hydrolase, at 4,093,648 to 4,094,346
_RS19580
BWI76_RS19585: BWI76_RS19585 - gentisate 1,2-dioxygenase, at 4,094,371 to 4,095,408
_RS19585
BWI76_RS19590: BWI76_RS19590 - MFS transporter, at 4,095,420 to 4,096,778
_RS19590
Position (kb)
4094
4095
4096 Strain fitness (log2 ratio)
-1
0
1 at 4093.886 kb on - strand, within BWI76_RS19580 at 4093.996 kb on + strand, within BWI76_RS19580 at 4094.384 kb on + strand at 4094.384 kb on + strand at 4094.553 kb on - strand, within BWI76_RS19585 at 4094.733 kb on - strand, within BWI76_RS19585 at 4094.905 kb on - strand, within BWI76_RS19585 at 4095.479 kb on + strand at 4095.500 kb on + strand at 4095.641 kb on - strand, within BWI76_RS19590 at 4095.812 kb on - strand, within BWI76_RS19590 at 4096.395 kb on - strand, within BWI76_RS19590
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 4,093,886 - BWI76_RS19580 0.34 -0.2 4,093,996 + BWI76_RS19580 0.50 -0.3 4,094,384 + +0.2 4,094,384 + +0.6 4,094,553 - BWI76_RS19585 0.18 -1.1 4,094,733 - BWI76_RS19585 0.35 -0.7 4,094,905 - BWI76_RS19585 0.51 -0.4 4,095,479 + -0.2 4,095,500 + -1.2 4,095,641 - BWI76_RS19590 0.16 -0.6 4,095,812 - BWI76_RS19590 0.29 +0.9 4,096,395 - BWI76_RS19590 0.72 -0.5
Or see this region's nucleotide sequence