Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16785 and BWI76_RS16790 are separated by 283 nucleotides BWI76_RS16790 and BWI76_RS16795 are separated by 100 nucleotides BWI76_RS16795 and BWI76_RS16800 overlap by 14 nucleotides
BWI76_RS16785: BWI76_RS16785 - peptidase M20, at 3,479,905 to 3,481,056
_RS16785
BWI76_RS16790: BWI76_RS16790 - MFS transporter, at 3,481,340 to 3,482,545
_RS16790
BWI76_RS16795: BWI76_RS16795 - amino acid ABC transporter ATPase, at 3,482,646 to 3,483,368
_RS16795
BWI76_RS16800: BWI76_RS16800 - amino acid ABC transporter permease, at 3,483,355 to 3,484,119
_RS16800
Position (kb)
3481
3482
3483 Strain fitness (log2 ratio)
-1
0
1 at 3480.509 kb on - strand, within BWI76_RS16785 at 3480.580 kb on + strand, within BWI76_RS16785 at 3481.092 kb on - strand at 3481.448 kb on - strand at 3481.739 kb on + strand, within BWI76_RS16790 at 3482.057 kb on - strand, within BWI76_RS16790 at 3482.057 kb on - strand, within BWI76_RS16790 at 3482.144 kb on + strand, within BWI76_RS16790 at 3482.144 kb on + strand, within BWI76_RS16790 at 3482.144 kb on + strand, within BWI76_RS16790 at 3482.258 kb on + strand, within BWI76_RS16790 at 3482.537 kb on + strand at 3482.553 kb on - strand at 3482.718 kb on - strand at 3482.878 kb on - strand, within BWI76_RS16795 at 3482.894 kb on + strand, within BWI76_RS16795 at 3483.505 kb on - strand, within BWI76_RS16800
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 3,480,509 - BWI76_RS16785 0.52 +0.6 3,480,580 + BWI76_RS16785 0.59 +0.4 3,481,092 - +0.4 3,481,448 - -0.1 3,481,739 + BWI76_RS16790 0.33 -0.6 3,482,057 - BWI76_RS16790 0.59 -1.1 3,482,057 - BWI76_RS16790 0.59 -1.3 3,482,144 + BWI76_RS16790 0.67 -0.3 3,482,144 + BWI76_RS16790 0.67 -0.1 3,482,144 + BWI76_RS16790 0.67 -0.4 3,482,258 + BWI76_RS16790 0.76 -0.5 3,482,537 + +0.5 3,482,553 - +0.1 3,482,718 - -0.1 3,482,878 - BWI76_RS16795 0.32 -0.0 3,482,894 + BWI76_RS16795 0.34 -0.4 3,483,505 - BWI76_RS16800 0.20 +0.4
Or see this region's nucleotide sequence