Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15220 and BWI76_RS15225 are separated by 12 nucleotides BWI76_RS15225 and BWI76_RS15230 are separated by 1 nucleotides BWI76_RS15230 and BWI76_RS15235 overlap by 8 nucleotides
BWI76_RS15220: BWI76_RS15220 - L-lactate dehydrogenase, at 3,190,094 to 3,191,038
_RS15220
BWI76_RS15225: BWI76_RS15225 - DeoR family transcriptional regulator, at 3,191,051 to 3,191,812
_RS15225
BWI76_RS15230: BWI76_RS15230 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 3,191,814 to 3,192,800
_RS15230
BWI76_RS15235: BWI76_RS15235 - hypothetical protein, at 3,192,793 to 3,194,031
_RS15235
Position (kb)
3191
3192 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3190.233 kb on + strand, within BWI76_RS15220 at 3190.611 kb on + strand, within BWI76_RS15220 at 3190.619 kb on - strand, within BWI76_RS15220 at 3191.144 kb on + strand, within BWI76_RS15225 at 3191.152 kb on - strand, within BWI76_RS15225 at 3191.276 kb on - strand, within BWI76_RS15225 at 3191.685 kb on + strand, within BWI76_RS15225 at 3191.693 kb on - strand, within BWI76_RS15225 at 3191.848 kb on - strand at 3191.851 kb on - strand at 3192.218 kb on + strand, within BWI76_RS15230 at 3192.243 kb on - strand, within BWI76_RS15230 at 3192.280 kb on + strand, within BWI76_RS15230 at 3192.316 kb on + strand, within BWI76_RS15230 at 3192.592 kb on + strand, within BWI76_RS15230 at 3192.728 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 3,190,233 + BWI76_RS15220 0.15 +0.4 3,190,611 + BWI76_RS15220 0.55 -1.3 3,190,619 - BWI76_RS15220 0.56 +0.5 3,191,144 + BWI76_RS15225 0.12 -1.8 3,191,152 - BWI76_RS15225 0.13 -4.5 3,191,276 - BWI76_RS15225 0.30 -0.5 3,191,685 + BWI76_RS15225 0.83 -2.4 3,191,693 - BWI76_RS15225 0.84 -3.6 3,191,848 - +2.0 3,191,851 - +0.0 3,192,218 + BWI76_RS15230 0.41 +0.4 3,192,243 - BWI76_RS15230 0.43 +0.2 3,192,280 + BWI76_RS15230 0.47 -0.3 3,192,316 + BWI76_RS15230 0.51 +0.6 3,192,592 + BWI76_RS15230 0.79 -0.9 3,192,728 - +0.3
Or see this region's nucleotide sequence