Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15205 and BWI76_RS15210 are separated by 105 nucleotides BWI76_RS15210 and BWI76_RS15215 overlap by 47 nucleotides BWI76_RS15215 and BWI76_RS15220 are separated by 145 nucleotides
BWI76_RS15205: BWI76_RS15205 - succinate-semialdehyde dehydrogenase, at 3,187,143 to 3,188,528
_RS15205
BWI76_RS15210: BWI76_RS15210 - LysR family transcriptional regulator, at 3,188,634 to 3,189,512
_RS15210
BWI76_RS15215: BWI76_RS15215 - N-acetyltransferase, at 3,189,466 to 3,189,948
_RS15215
BWI76_RS15220: BWI76_RS15220 - L-lactate dehydrogenase, at 3,190,094 to 3,191,038
_RS15220
Position (kb) 3189
3190 Strain fitness (log2 ratio) -1
0
1 at 3188.691 kb on - strand at 3188.970 kb on - strand, within BWI76_RS15210 at 3189.178 kb on + strand, within BWI76_RS15210 at 3189.322 kb on + strand, within BWI76_RS15210 at 3189.351 kb on - strand, within BWI76_RS15210 at 3189.595 kb on + strand, within BWI76_RS15215 at 3190.233 kb on + strand, within BWI76_RS15220 at 3190.611 kb on + strand, within BWI76_RS15220 at 3190.619 kb on - strand, within BWI76_RS15220
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 3,188,691 - +0.3 3,188,970 - BWI76_RS15210 0.38 -0.7 3,189,178 + BWI76_RS15210 0.62 +0.1 3,189,322 + BWI76_RS15210 0.78 +0.5 3,189,351 - BWI76_RS15210 0.82 +0.8 3,189,595 + BWI76_RS15215 0.27 -0.9 3,190,233 + BWI76_RS15220 0.15 +0.4 3,190,611 + BWI76_RS15220 0.55 -1.3 3,190,619 - BWI76_RS15220 0.56 +0.5
Or see this region's nucleotide sequence