Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12590 and BWI76_RS12595 are separated by 99 nucleotides BWI76_RS12595 and BWI76_RS12600 are separated by 81 nucleotides BWI76_RS12600 and BWI76_RS12605 are separated by 198 nucleotides
BWI76_RS12590: BWI76_RS12590 - helix-turn-helix-type transcriptional regulator, at 2,628,126 to 2,628,854
_RS12590
BWI76_RS12595: BWI76_RS12595 - hypothetical protein, at 2,628,954 to 2,629,298
_RS12595
BWI76_RS12600: BWI76_RS12600 - MFS transporter, at 2,629,380 to 2,630,501
_RS12600
BWI76_RS12605: BWI76_RS12605 - hypothetical protein, at 2,630,700 to 2,631,836
_RS12605
Position (kb)
2629
2630
2631 Strain fitness (log2 ratio)
-1
0
1 at 2628.400 kb on - strand, within BWI76_RS12590 at 2628.416 kb on - strand, within BWI76_RS12590 at 2628.484 kb on - strand, within BWI76_RS12590 at 2628.771 kb on - strand, within BWI76_RS12590 at 2628.771 kb on - strand, within BWI76_RS12590 at 2629.141 kb on - strand, within BWI76_RS12595 at 2629.198 kb on + strand, within BWI76_RS12595 at 2629.230 kb on - strand, within BWI76_RS12595 at 2629.282 kb on - strand at 2629.372 kb on + strand at 2629.380 kb on - strand at 2629.819 kb on - strand, within BWI76_RS12600 at 2629.864 kb on - strand, within BWI76_RS12600 at 2629.927 kb on + strand, within BWI76_RS12600 at 2630.077 kb on - strand, within BWI76_RS12600 at 2630.215 kb on + strand, within BWI76_RS12600 at 2630.361 kb on + strand, within BWI76_RS12600 at 2630.394 kb on + strand at 2630.402 kb on - strand at 2630.553 kb on + strand at 2630.689 kb on + strand at 2630.763 kb on + strand at 2630.883 kb on - strand, within BWI76_RS12605 at 2631.151 kb on + strand, within BWI76_RS12605 at 2631.177 kb on + strand, within BWI76_RS12605
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 2,628,400 - BWI76_RS12590 0.38 +0.2 2,628,416 - BWI76_RS12590 0.40 -0.9 2,628,484 - BWI76_RS12590 0.49 +0.7 2,628,771 - BWI76_RS12590 0.88 -1.3 2,628,771 - BWI76_RS12590 0.88 -1.7 2,629,141 - BWI76_RS12595 0.54 +0.1 2,629,198 + BWI76_RS12595 0.71 -0.6 2,629,230 - BWI76_RS12595 0.80 -0.5 2,629,282 - +1.3 2,629,372 + +0.3 2,629,380 - +0.5 2,629,819 - BWI76_RS12600 0.39 -1.5 2,629,864 - BWI76_RS12600 0.43 -0.9 2,629,927 + BWI76_RS12600 0.49 -1.2 2,630,077 - BWI76_RS12600 0.62 +0.7 2,630,215 + BWI76_RS12600 0.74 -1.1 2,630,361 + BWI76_RS12600 0.87 -0.3 2,630,394 + +0.2 2,630,402 - +0.6 2,630,553 + -0.2 2,630,689 + -0.4 2,630,763 + -0.0 2,630,883 - BWI76_RS12605 0.16 -0.6 2,631,151 + BWI76_RS12605 0.40 -0.4 2,631,177 + BWI76_RS12605 0.42 +1.0
Or see this region's nucleotide sequence