Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06520 and BWI76_RS06525 are separated by 316 nucleotides BWI76_RS06525 and BWI76_RS06530 are separated by 132 nucleotides
BWI76_RS06520: BWI76_RS06520 - trigger factor, at 1,368,872 to 1,370,170
_RS06520
BWI76_RS06525: BWI76_RS06525 - ATP-dependent Clp protease proteolytic subunit, at 1,370,487 to 1,371,110
_RS06525
BWI76_RS06530: BWI76_RS06530 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 1,371,243 to 1,372,517
_RS06530
Position (kb)
1370
1371
1372 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1369.782 kb on - strand, within BWI76_RS06520 at 1369.865 kb on - strand, within BWI76_RS06520 at 1369.900 kb on - strand, within BWI76_RS06520 at 1370.142 kb on + strand at 1370.182 kb on + strand at 1370.182 kb on + strand at 1370.369 kb on + strand at 1370.379 kb on - strand at 1370.397 kb on - strand at 1370.402 kb on - strand at 1370.523 kb on + strand at 1370.651 kb on + strand, within BWI76_RS06525 at 1370.854 kb on - strand, within BWI76_RS06525 at 1370.987 kb on - strand, within BWI76_RS06525 at 1371.000 kb on + strand, within BWI76_RS06525 at 1371.007 kb on + strand, within BWI76_RS06525 at 1371.232 kb on + strand at 1371.276 kb on + strand at 1371.435 kb on - strand, within BWI76_RS06530 at 1371.570 kb on - strand, within BWI76_RS06530 at 1371.705 kb on + strand, within BWI76_RS06530 at 1371.748 kb on - strand, within BWI76_RS06530
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 1,369,782 - BWI76_RS06520 0.70 -0.0 1,369,865 - BWI76_RS06520 0.76 +0.2 1,369,900 - BWI76_RS06520 0.79 -0.1 1,370,142 + +1.2 1,370,182 + -1.3 1,370,182 + -0.2 1,370,369 + -0.8 1,370,379 - -0.7 1,370,397 - +0.2 1,370,402 - -0.8 1,370,523 + -2.5 1,370,651 + BWI76_RS06525 0.26 -2.4 1,370,854 - BWI76_RS06525 0.59 -2.3 1,370,987 - BWI76_RS06525 0.80 -2.0 1,371,000 + BWI76_RS06525 0.82 -2.1 1,371,007 + BWI76_RS06525 0.83 -4.2 1,371,232 + -1.6 1,371,276 + -1.4 1,371,435 - BWI76_RS06530 0.15 -1.0 1,371,570 - BWI76_RS06530 0.26 -2.3 1,371,705 + BWI76_RS06530 0.36 -1.2 1,371,748 - BWI76_RS06530 0.40 -0.9
Or see this region's nucleotide sequence