Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06165 and BWI76_RS06170 are separated by 46 nucleotides BWI76_RS06170 and BWI76_RS06175 are separated by 116 nucleotides BWI76_RS06175 and BWI76_RS06180 overlap by 4 nucleotides
BWI76_RS06165: BWI76_RS06165 - phosphate starvation-inducible protein PsiF, at 1,295,596 to 1,295,916
_RS06165
BWI76_RS06170: BWI76_RS06170 - pyrroline-5-carboxylate reductase, at 1,295,963 to 1,296,772
_RS06170
BWI76_RS06175: BWI76_RS06175 - DUF188 domain-containing protein, at 1,296,889 to 1,297,347
_RS06175
BWI76_RS06180: BWI76_RS06180 - hypothetical protein, at 1,297,344 to 1,298,042
_RS06180
Position (kb)
1296
1297
1298 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1296.958 kb on - strand, within BWI76_RS06175 at 1297.039 kb on + strand, within BWI76_RS06175 at 1297.173 kb on + strand, within BWI76_RS06175 at 1297.405 kb on + strand at 1297.546 kb on - strand, within BWI76_RS06180 at 1297.580 kb on - strand, within BWI76_RS06180 at 1297.853 kb on + strand, within BWI76_RS06180 at 1298.038 kb on - strand at 1298.039 kb on + strand at 1298.052 kb on - strand at 1298.125 kb on - strand at 1298.125 kb on - strand at 1298.125 kb on - strand at 1298.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 1,296,958 - BWI76_RS06175 0.15 -1.0 1,297,039 + BWI76_RS06175 0.33 +0.3 1,297,173 + BWI76_RS06175 0.62 -3.4 1,297,405 + +0.2 1,297,546 - BWI76_RS06180 0.29 -0.6 1,297,580 - BWI76_RS06180 0.34 -0.2 1,297,853 + BWI76_RS06180 0.73 -0.2 1,298,038 - +0.2 1,298,039 + +0.0 1,298,052 - -1.3 1,298,125 - +0.8 1,298,125 - +0.4 1,298,125 - +0.3 1,298,321 - +0.1
Or see this region's nucleotide sequence