Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02810 and BWI76_RS02815 are separated by 44 nucleotides BWI76_RS02815 and BWI76_RS02820 are separated by 175 nucleotides
BWI76_RS02810: BWI76_RS02810 - sugar ABC transporter permease YjfF, at 573,817 to 574,803
_RS02810
BWI76_RS02815: BWI76_RS02815 - fructose-bisphosphatase class I, at 574,848 to 575,846
_RS02815
BWI76_RS02820: BWI76_RS02820 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 576,022 to 577,395
_RS02820
Position (kb)
574
575
576 Strain fitness (log2 ratio)
-2
-1
0
1 at 573.881 kb on - strand at 574.280 kb on - strand, within BWI76_RS02810 at 574.280 kb on - strand, within BWI76_RS02810 at 574.549 kb on - strand, within BWI76_RS02810 at 574.598 kb on + strand, within BWI76_RS02810 at 574.690 kb on - strand, within BWI76_RS02810 at 574.892 kb on - strand at 575.069 kb on + strand, within BWI76_RS02815 at 575.351 kb on - strand, within BWI76_RS02815 at 575.631 kb on - strand, within BWI76_RS02815 at 575.769 kb on + strand at 575.865 kb on - strand at 575.922 kb on + strand at 575.928 kb on - strand at 575.979 kb on + strand at 576.100 kb on - strand at 576.100 kb on - strand at 576.128 kb on - strand at 576.546 kb on + strand, within BWI76_RS02820 at 576.731 kb on + strand, within BWI76_RS02820 at 576.739 kb on - strand, within BWI76_RS02820 at 576.792 kb on + strand, within BWI76_RS02820
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 573,881 - +0.1 574,280 - BWI76_RS02810 0.47 +0.1 574,280 - BWI76_RS02810 0.47 -2.2 574,549 - BWI76_RS02810 0.74 +1.0 574,598 + BWI76_RS02810 0.79 +0.5 574,690 - BWI76_RS02810 0.88 +0.2 574,892 - +0.4 575,069 + BWI76_RS02815 0.22 -0.6 575,351 - BWI76_RS02815 0.50 -1.0 575,631 - BWI76_RS02815 0.78 -1.5 575,769 + -0.1 575,865 - -0.2 575,922 + -0.1 575,928 - -2.0 575,979 + -0.4 576,100 - -0.3 576,100 - -1.1 576,128 - -1.6 576,546 + BWI76_RS02820 0.38 -1.0 576,731 + BWI76_RS02820 0.52 +0.2 576,739 - BWI76_RS02820 0.52 +0.7 576,792 + BWI76_RS02820 0.56 +0.3
Or see this region's nucleotide sequence