Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01085 and BWI76_RS01090 are separated by 56 nucleotides BWI76_RS01090 and BWI76_RS01095 overlap by 4 nucleotides
BWI76_RS01085: BWI76_RS01085 - polysaccharide chain length modulation protein, at 223,330 to 224,376
_RS01085
BWI76_RS01090: BWI76_RS01090 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 224,433 to 225,563
_RS01090
BWI76_RS01095: BWI76_RS01095 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 225,560 to 226,822
_RS01095
Position (kb) 224
225
226 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 223.438 kb on + strand, within BWI76_RS01085 at 223.608 kb on + strand, within BWI76_RS01085 at 223.697 kb on + strand, within BWI76_RS01085 at 223.917 kb on + strand, within BWI76_RS01085 at 224.006 kb on + strand, within BWI76_RS01085 at 224.069 kb on + strand, within BWI76_RS01085 at 224.097 kb on + strand, within BWI76_RS01085 at 224.119 kb on - strand, within BWI76_RS01085 at 224.184 kb on - strand, within BWI76_RS01085 at 224.637 kb on - strand, within BWI76_RS01090 at 224.826 kb on - strand, within BWI76_RS01090 at 224.859 kb on + strand, within BWI76_RS01090 at 225.000 kb on - strand, within BWI76_RS01090 at 225.010 kb on + strand, within BWI76_RS01090 at 225.020 kb on + strand, within BWI76_RS01090 at 225.502 kb on + strand at 225.589 kb on + strand at 226.034 kb on - strand, within BWI76_RS01095 at 226.533 kb on + strand, within BWI76_RS01095 at 226.541 kb on - strand, within BWI76_RS01095
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 223,438 + BWI76_RS01085 0.10 -0.7 223,608 + BWI76_RS01085 0.27 +1.1 223,697 + BWI76_RS01085 0.35 -1.1 223,917 + BWI76_RS01085 0.56 -1.2 224,006 + BWI76_RS01085 0.65 -1.3 224,069 + BWI76_RS01085 0.71 -0.9 224,097 + BWI76_RS01085 0.73 -1.5 224,119 - BWI76_RS01085 0.75 +0.1 224,184 - BWI76_RS01085 0.82 -3.6 224,637 - BWI76_RS01090 0.18 +1.5 224,826 - BWI76_RS01090 0.35 -0.4 224,859 + BWI76_RS01090 0.38 -1.5 225,000 - BWI76_RS01090 0.50 -1.4 225,010 + BWI76_RS01090 0.51 -1.7 225,020 + BWI76_RS01090 0.52 -1.5 225,502 + -0.7 225,589 + -0.7 226,034 - BWI76_RS01095 0.38 +0.1 226,533 + BWI76_RS01095 0.77 -0.8 226,541 - BWI76_RS01095 0.78 -0.3
Or see this region's nucleotide sequence