Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00600 and BWI76_RS00605 are separated by 114 nucleotides BWI76_RS00605 and BWI76_RS00610 are separated by 41 nucleotides
BWI76_RS00600: BWI76_RS00600 - LacI family transcriptional regulator, at 119,728 to 120,723
_RS00600
BWI76_RS00605: BWI76_RS00605 - alpha-galactosidase, at 120,838 to 122,961
_RS00605
BWI76_RS00610: BWI76_RS00610 - MFS transporter, at 123,003 to 124,268
_RS00610
Position (kb) 120
121
122
123 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 119.850 kb on - strand, within BWI76_RS00600 at 119.890 kb on - strand, within BWI76_RS00600 at 120.058 kb on - strand, within BWI76_RS00600 at 120.072 kb on - strand, within BWI76_RS00600 at 120.072 kb on - strand, within BWI76_RS00600 at 120.118 kb on - strand, within BWI76_RS00600 at 120.418 kb on - strand, within BWI76_RS00600 at 120.613 kb on + strand, within BWI76_RS00600 at 121.130 kb on + strand, within BWI76_RS00605 at 121.201 kb on + strand, within BWI76_RS00605 at 121.222 kb on + strand, within BWI76_RS00605 at 121.230 kb on - strand, within BWI76_RS00605 at 121.284 kb on + strand, within BWI76_RS00605 at 121.458 kb on + strand, within BWI76_RS00605 at 121.644 kb on - strand, within BWI76_RS00605 at 121.654 kb on - strand, within BWI76_RS00605 at 121.720 kb on + strand, within BWI76_RS00605 at 121.765 kb on - strand, within BWI76_RS00605 at 121.832 kb on + strand, within BWI76_RS00605 at 121.913 kb on + strand, within BWI76_RS00605 at 121.990 kb on + strand, within BWI76_RS00605 at 121.998 kb on - strand, within BWI76_RS00605 at 121.998 kb on - strand, within BWI76_RS00605 at 122.033 kb on + strand, within BWI76_RS00605 at 122.147 kb on + strand, within BWI76_RS00605 at 122.179 kb on - strand at 122.193 kb on + strand, within BWI76_RS00605 at 122.288 kb on - strand, within BWI76_RS00605 at 122.317 kb on + strand, within BWI76_RS00605 at 122.337 kb on + strand, within BWI76_RS00605 at 122.378 kb on + strand, within BWI76_RS00605 at 122.477 kb on - strand, within BWI76_RS00605 at 122.861 kb on - strand at 122.986 kb on + strand at 123.057 kb on - strand at 123.127 kb on + strand at 123.135 kb on - strand, within BWI76_RS00610 at 123.162 kb on - strand, within BWI76_RS00610 at 123.204 kb on - strand, within BWI76_RS00610 at 123.231 kb on - strand, within BWI76_RS00610 at 123.500 kb on + strand, within BWI76_RS00610 at 123.500 kb on + strand, within BWI76_RS00610 at 123.505 kb on + strand, within BWI76_RS00610 at 123.508 kb on - strand, within BWI76_RS00610 at 123.590 kb on - strand, within BWI76_RS00610 at 123.598 kb on + strand, within BWI76_RS00610 at 123.598 kb on - strand, within BWI76_RS00610 at 123.625 kb on - strand, within BWI76_RS00610 at 123.796 kb on + strand, within BWI76_RS00610 at 123.855 kb on + strand, within BWI76_RS00610
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 119,850 - BWI76_RS00600 0.12 +1.5 119,890 - BWI76_RS00600 0.16 -0.4 120,058 - BWI76_RS00600 0.33 -0.9 120,072 - BWI76_RS00600 0.35 -1.6 120,072 - BWI76_RS00600 0.35 -0.6 120,118 - BWI76_RS00600 0.39 -2.8 120,418 - BWI76_RS00600 0.69 -0.9 120,613 + BWI76_RS00600 0.89 -0.5 121,130 + BWI76_RS00605 0.14 +0.4 121,201 + BWI76_RS00605 0.17 +0.8 121,222 + BWI76_RS00605 0.18 +0.2 121,230 - BWI76_RS00605 0.18 +2.3 121,284 + BWI76_RS00605 0.21 +0.7 121,458 + BWI76_RS00605 0.29 +0.3 121,644 - BWI76_RS00605 0.38 -0.7 121,654 - BWI76_RS00605 0.38 -0.2 121,720 + BWI76_RS00605 0.42 -0.2 121,765 - BWI76_RS00605 0.44 +0.7 121,832 + BWI76_RS00605 0.47 +1.4 121,913 + BWI76_RS00605 0.51 +0.5 121,990 + BWI76_RS00605 0.54 +0.3 121,998 - BWI76_RS00605 0.55 +0.2 121,998 - BWI76_RS00605 0.55 +0.6 122,033 + BWI76_RS00605 0.56 +0.5 122,147 + BWI76_RS00605 0.62 +0.5 122,179 - +0.9 122,193 + BWI76_RS00605 0.64 -0.1 122,288 - BWI76_RS00605 0.68 +0.1 122,317 + BWI76_RS00605 0.70 +2.1 122,337 + BWI76_RS00605 0.71 -0.3 122,378 + BWI76_RS00605 0.73 -0.0 122,477 - BWI76_RS00605 0.77 +0.4 122,861 - +0.2 122,986 + -0.0 123,057 - -0.6 123,127 + +0.9 123,135 - BWI76_RS00610 0.10 -0.1 123,162 - BWI76_RS00610 0.13 -2.0 123,204 - BWI76_RS00610 0.16 -0.6 123,231 - BWI76_RS00610 0.18 -1.3 123,500 + BWI76_RS00610 0.39 -0.6 123,500 + BWI76_RS00610 0.39 +0.2 123,505 + BWI76_RS00610 0.40 -0.1 123,508 - BWI76_RS00610 0.40 -0.4 123,590 - BWI76_RS00610 0.46 +0.3 123,598 + BWI76_RS00610 0.47 +0.1 123,598 - BWI76_RS00610 0.47 +0.6 123,625 - BWI76_RS00610 0.49 -0.2 123,796 + BWI76_RS00610 0.63 -0.4 123,855 + BWI76_RS00610 0.67 +0.3
Or see this region's nucleotide sequence