Experiment: Pyruvate-Sulfite (60-20mM) with Molybdate 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acrA and DVU2818 are separated by 74 nucleotides DVU2818 and DVU2819 are separated by 163 nucleotides DVU2819 and DVU2820 are separated by 187 nucleotides
DVU2817: acrA - multidrug resistance protein (TIGR), at 2,923,238 to 2,924,491
acrA
DVU2818: DVU2818 - hypothetical protein (TIGR), at 2,924,566 to 2,924,784
DVU2818
DVU2819: DVU2819 - transcriptional regulator, TetR family (TIGR), at 2,924,948 to 2,925,556
DVU2819
DVU2820: DVU2820 - amidohydrolase family protein (TIGR), at 2,925,744 to 2,926,580
DVU2820
Position (kb)
2924
2925
2926 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2923.966 kb on - strand, within acrA at 2923.991 kb on + strand, within acrA at 2924.020 kb on - strand, within acrA at 2924.034 kb on + strand, within acrA at 2924.034 kb on + strand, within acrA at 2924.034 kb on + strand, within acrA at 2924.042 kb on - strand, within acrA at 2924.042 kb on - strand, within acrA at 2924.195 kb on + strand, within acrA at 2924.219 kb on - strand, within acrA at 2924.337 kb on + strand, within acrA at 2924.345 kb on - strand, within acrA at 2924.494 kb on + strand at 2924.497 kb on - strand at 2924.502 kb on - strand at 2924.528 kb on + strand at 2924.603 kb on - strand, within DVU2818 at 2924.603 kb on - strand, within DVU2818 at 2924.649 kb on + strand, within DVU2818 at 2924.718 kb on + strand, within DVU2818 at 2924.894 kb on - strand at 2924.903 kb on - strand at 2924.936 kb on + strand at 2924.938 kb on + strand at 2924.951 kb on + strand at 2925.018 kb on - strand, within DVU2819 at 2925.083 kb on + strand, within DVU2819 at 2925.083 kb on + strand, within DVU2819 at 2925.208 kb on - strand, within DVU2819 at 2925.361 kb on + strand, within DVU2819 at 2925.436 kb on + strand, within DVU2819 at 2925.436 kb on + strand, within DVU2819 at 2925.451 kb on + strand, within DVU2819 at 2925.565 kb on + strand at 2925.573 kb on - strand at 2925.577 kb on - strand at 2925.607 kb on + strand at 2925.607 kb on + strand at 2925.607 kb on + strand at 2925.610 kb on - strand at 2925.610 kb on - strand at 2925.620 kb on - strand at 2925.641 kb on - strand at 2925.648 kb on + strand at 2925.764 kb on + strand at 2925.830 kb on + strand, within DVU2820 at 2925.838 kb on - strand, within DVU2820 at 2925.838 kb on - strand, within DVU2820 at 2925.843 kb on + strand, within DVU2820 at 2925.932 kb on - strand, within DVU2820 at 2925.966 kb on - strand, within DVU2820 at 2925.984 kb on + strand, within DVU2820 at 2926.023 kb on + strand, within DVU2820 at 2926.068 kb on - strand, within DVU2820 at 2926.084 kb on + strand, within DVU2820 at 2926.087 kb on - strand, within DVU2820 at 2926.092 kb on - strand, within DVU2820 at 2926.107 kb on - strand, within DVU2820 at 2926.145 kb on + strand, within DVU2820 at 2926.153 kb on - strand, within DVU2820 at 2926.173 kb on - strand, within DVU2820 at 2926.182 kb on - strand, within DVU2820 at 2926.195 kb on + strand, within DVU2820 at 2926.273 kb on - strand, within DVU2820 at 2926.278 kb on + strand, within DVU2820 at 2926.295 kb on + strand, within DVU2820 at 2926.308 kb on + strand, within DVU2820 at 2926.384 kb on + strand, within DVU2820 at 2926.404 kb on - strand, within DVU2820
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Sulfite (60-20mM) with Molybdate 100uM remove 2,923,966 - acrA DVU2817 0.58 -0.2 2,923,991 + acrA DVU2817 0.60 -0.2 2,924,020 - acrA DVU2817 0.62 -2.4 2,924,034 + acrA DVU2817 0.63 +1.0 2,924,034 + acrA DVU2817 0.63 -0.1 2,924,034 + acrA DVU2817 0.63 -0.1 2,924,042 - acrA DVU2817 0.64 -0.0 2,924,042 - acrA DVU2817 0.64 +0.2 2,924,195 + acrA DVU2817 0.76 -0.2 2,924,219 - acrA DVU2817 0.78 +0.5 2,924,337 + acrA DVU2817 0.88 -0.1 2,924,345 - acrA DVU2817 0.88 +0.4 2,924,494 + -0.6 2,924,497 - +0.8 2,924,502 - -0.2 2,924,528 + -0.1 2,924,603 - DVU2818 0.17 -0.4 2,924,603 - DVU2818 0.17 -0.7 2,924,649 + DVU2818 0.38 +0.2 2,924,718 + DVU2818 0.69 -0.1 2,924,894 - -0.1 2,924,903 - +0.1 2,924,936 + +0.5 2,924,938 + -0.2 2,924,951 + -0.4 2,925,018 - DVU2819 0.11 -1.4 2,925,083 + DVU2819 0.22 -0.8 2,925,083 + DVU2819 0.22 -0.7 2,925,208 - DVU2819 0.43 -2.2 2,925,361 + DVU2819 0.68 -0.5 2,925,436 + DVU2819 0.80 -1.4 2,925,436 + DVU2819 0.80 -0.5 2,925,451 + DVU2819 0.83 -1.6 2,925,565 + -1.9 2,925,573 - -0.5 2,925,577 - -0.2 2,925,607 + -0.5 2,925,607 + -1.1 2,925,607 + +0.4 2,925,610 - -0.4 2,925,610 - -1.1 2,925,620 - -0.1 2,925,641 - -0.1 2,925,648 + -0.5 2,925,764 + +0.2 2,925,830 + DVU2820 0.10 -0.5 2,925,838 - DVU2820 0.11 +0.8 2,925,838 - DVU2820 0.11 +1.9 2,925,843 + DVU2820 0.12 -0.1 2,925,932 - DVU2820 0.22 -0.6 2,925,966 - DVU2820 0.27 -1.1 2,925,984 + DVU2820 0.29 +0.1 2,926,023 + DVU2820 0.33 -0.4 2,926,068 - DVU2820 0.39 -0.1 2,926,084 + DVU2820 0.41 -2.6 2,926,087 - DVU2820 0.41 -0.9 2,926,092 - DVU2820 0.42 +1.2 2,926,107 - DVU2820 0.43 -0.2 2,926,145 + DVU2820 0.48 +0.1 2,926,153 - DVU2820 0.49 -0.1 2,926,173 - DVU2820 0.51 -2.0 2,926,182 - DVU2820 0.52 +0.9 2,926,195 + DVU2820 0.54 +0.1 2,926,273 - DVU2820 0.63 +0.2 2,926,278 + DVU2820 0.64 -0.1 2,926,295 + DVU2820 0.66 -0.4 2,926,308 + DVU2820 0.67 +0.7 2,926,384 + DVU2820 0.76 -0.3 2,926,404 - DVU2820 0.79 -0.3
Or see this region's nucleotide sequence