Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5478 and Pf6N2E2_5479 overlap by 4 nucleotides Pf6N2E2_5479 and Pf6N2E2_5480 are separated by 15 nucleotides
Pf6N2E2_5478 - ABC spermidine/putrescine transporter, inner membrane subunit, at 6,296,331 to 6,297,197
_5478
Pf6N2E2_5479 - ABC spermidine/putrescine transporter, inner membrane subunit, at 6,297,194 to 6,298,042
_5479
Pf6N2E2_5480 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 6,298,058 to 6,299,113
_5480
Position (kb)
6297
6298
6299 Strain fitness (log2 ratio)
-1
0
1 at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.349 kb on - strand at 6296.491 kb on - strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.514 kb on + strand, within Pf6N2E2_5478 at 6296.515 kb on - strand, within Pf6N2E2_5478 at 6296.515 kb on - strand, within Pf6N2E2_5478 at 6296.596 kb on - strand, within Pf6N2E2_5478 at 6296.596 kb on - strand, within Pf6N2E2_5478 at 6296.688 kb on + strand, within Pf6N2E2_5478 at 6296.688 kb on + strand, within Pf6N2E2_5478 at 6296.689 kb on - strand, within Pf6N2E2_5478 at 6296.689 kb on - strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.985 kb on + strand, within Pf6N2E2_5478 at 6296.986 kb on - strand, within Pf6N2E2_5478 at 6297.153 kb on + strand at 6297.360 kb on - strand, within Pf6N2E2_5479 at 6297.872 kb on + strand, within Pf6N2E2_5479 at 6297.872 kb on + strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.881 kb on + strand, within Pf6N2E2_5479 at 6297.881 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.918 kb on - strand, within Pf6N2E2_5479 at 6297.918 kb on - strand, within Pf6N2E2_5479 at 6298.071 kb on + strand at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.722 kb on - strand, within Pf6N2E2_5480 at 6299.026 kb on + strand at 6299.026 kb on + strand at 6299.027 kb on - strand at 6299.027 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 6,296,348 + -0.1 6,296,348 + -0.8 6,296,348 + +0.3 6,296,348 + -0.2 6,296,349 - +1.1 6,296,491 - Pf6N2E2_5478 0.18 +1.3 6,296,511 + Pf6N2E2_5478 0.21 -1.1 6,296,511 + Pf6N2E2_5478 0.21 -0.2 6,296,511 + Pf6N2E2_5478 0.21 +0.0 6,296,512 - Pf6N2E2_5478 0.21 -0.9 6,296,512 - Pf6N2E2_5478 0.21 +1.4 6,296,512 - Pf6N2E2_5478 0.21 +1.3 6,296,512 - Pf6N2E2_5478 0.21 -0.1 6,296,514 + Pf6N2E2_5478 0.21 +0.3 6,296,515 - Pf6N2E2_5478 0.21 -0.7 6,296,515 - Pf6N2E2_5478 0.21 -1.3 6,296,596 - Pf6N2E2_5478 0.31 +0.9 6,296,596 - Pf6N2E2_5478 0.31 +0.1 6,296,688 + Pf6N2E2_5478 0.41 -0.4 6,296,688 + Pf6N2E2_5478 0.41 -0.5 6,296,689 - Pf6N2E2_5478 0.41 -0.0 6,296,689 - Pf6N2E2_5478 0.41 +1.4 6,296,778 + Pf6N2E2_5478 0.52 +0.8 6,296,778 + Pf6N2E2_5478 0.52 -0.3 6,296,778 + Pf6N2E2_5478 0.52 +0.9 6,296,778 + Pf6N2E2_5478 0.52 -0.5 6,296,778 + Pf6N2E2_5478 0.52 -1.6 6,296,779 - Pf6N2E2_5478 0.52 -0.2 6,296,779 - Pf6N2E2_5478 0.52 -1.0 6,296,779 - Pf6N2E2_5478 0.52 -1.1 6,296,874 + Pf6N2E2_5478 0.63 +0.1 6,296,874 + Pf6N2E2_5478 0.63 +0.6 6,296,874 + Pf6N2E2_5478 0.63 -1.2 6,296,874 + Pf6N2E2_5478 0.63 +0.2 6,296,874 + Pf6N2E2_5478 0.63 +1.0 6,296,874 + Pf6N2E2_5478 0.63 -0.4 6,296,985 + Pf6N2E2_5478 0.75 +0.3 6,296,986 - Pf6N2E2_5478 0.76 +0.6 6,297,153 + +0.3 6,297,360 - Pf6N2E2_5479 0.20 +0.3 6,297,872 + Pf6N2E2_5479 0.80 -1.5 6,297,872 + Pf6N2E2_5479 0.80 +0.2 6,297,873 - Pf6N2E2_5479 0.80 -0.9 6,297,873 - Pf6N2E2_5479 0.80 -0.3 6,297,873 - Pf6N2E2_5479 0.80 -0.4 6,297,873 - Pf6N2E2_5479 0.80 -0.7 6,297,881 + Pf6N2E2_5479 0.81 -0.6 6,297,881 + Pf6N2E2_5479 0.81 -1.2 6,297,917 + Pf6N2E2_5479 0.85 -0.7 6,297,917 + Pf6N2E2_5479 0.85 +1.4 6,297,917 + Pf6N2E2_5479 0.85 -1.3 6,297,917 + Pf6N2E2_5479 0.85 -0.2 6,297,918 - Pf6N2E2_5479 0.85 -0.6 6,297,918 - Pf6N2E2_5479 0.85 +0.2 6,298,071 + -0.3 6,298,648 + Pf6N2E2_5480 0.56 -0.7 6,298,648 + Pf6N2E2_5480 0.56 +1.0 6,298,648 + Pf6N2E2_5480 0.56 +0.4 6,298,648 + Pf6N2E2_5480 0.56 -1.1 6,298,648 + Pf6N2E2_5480 0.56 -0.1 6,298,649 - Pf6N2E2_5480 0.56 -0.2 6,298,649 - Pf6N2E2_5480 0.56 +1.3 6,298,649 - Pf6N2E2_5480 0.56 +1.0 6,298,649 - Pf6N2E2_5480 0.56 -0.7 6,298,649 - Pf6N2E2_5480 0.56 +1.0 6,298,722 - Pf6N2E2_5480 0.63 -0.4 6,299,026 + -0.0 6,299,026 + +0.1 6,299,027 - +0.1 6,299,027 - +0.7
Or see this region's nucleotide sequence